Vojtěch Čermák

TL;DR: The results show that de novo DNA methylation is a rapid process initiated practically immediately with the appearance of promoter-specific siRNAs and independently of the prior presence of methylcytosines at the target locus.

TL;DR: The involvement of Group I bZIP TFs in the heat stress response (HSR) is not known as discussed by the authors, but they have been found to possess two motifs containing serine residues that are candidates for phosphorylation.

TL;DR: It is shown that even internal promotorless regions of T-DNAs are often transcribed, and this phenomenon can have significant consequences when working with highly sensitive systems like RNAi induction using an inverted repeat construct, so it should be generally considered when interpreting results obtained with the transgenic technology.

TL;DR: An improved protocol for BY-2 transformation and cloning is presented and the available plant cell lines methodology is extended toward high-throughput technologies like fluorescent-based cell sorting and transcriptomics.

TL;DR: In plants, posttranscriptional gene silencing (PTGS) is induced by small RNAs (sRNAs) generated from various dsRNA precursors, and DNA methylation accompanying PTGS was influenced both by the level and origin of sRNAs, and possibly also by the epigenetic state of the locus.