V
Volker Nölle
Researcher at Miltenyi Biotec
Publications - 20
Citations - 64
Volker Nölle is an academic researcher from Miltenyi Biotec. The author has contributed to research in topics: Nuclease & Medicine. The author has an hindex of 4, co-authored 17 publications receiving 46 citations.
Papers
More filters
Journal ArticleDOI
Purification of bone morphogenetic protein-2 from refolding mixtures using mixed-mode membrane chromatography
Gesa Gieseler,Iliyana Pepelanova,Lena Stuckenberg,Louis Villain,Volker Nölle,Uwe Odenthal,Sascha Beutel,Ursula Rinas,Thomas Scheper +8 more
TL;DR: Mixed-mode membrane chromatography was used to circumvent the use of heparin-affinity chromatography, thus allowing the design of an animal-component-free process for the purification of recombinant human bone morphogenetic protein-2.
Patent
Humanized antibody or fragment thereof specific for CD45R0
TL;DR: In this article, the authors provided a humanized antibody or fragment thereof specific for the antigen CD45R0 for enrichment or depletion of CD45r0-expressing cells.
Journal ArticleDOI
Bacterial non-specific nucleases of the phospholipase D superfamily and their biotechnological potential.
TL;DR: The classification and biotechnological potential of bacterial NSNs with a special focus on metal ion–independent nucleases are presented and discussed and PLD-family nucleases exhibit nucleolytic activity.
Journal ArticleDOI
A non-specific nucleolytic enzyme and its application potential in EDTA-containing buffer solutions
TL;DR: Proof-of-concept has been demonstrated that a metal-ion independent non-specific nuclease can be applied for removal of nucleic acids in EDTA-containing buffers for the subsequent purification of proteins from crude extracts.
Journal ArticleDOI
Comparative analysis of two non-specific nucleases of the phospholipase D family from the plant pathogen competitor bacterium Pantoea agglomerans
TL;DR: Different enzymatic properties suggest that the roles of PaNUC-1 and PaNuc-2 differ in the cell and might be the result of functional diversification after an ancient gene duplication event took place, which might pave the way to versatile biotechnological applications.