The aim of this study was to characterize variations in Raf kinase inhibitor protein (RKIP) expression and related signaling molecules in gastric cardia adenocarcinoma. Cancerous and precancerous tissues were collected from patients with gastric cardia adenocarcinoma and normal tissue was collected from healthy controls. RKIP expression was detected in these tissues and the serum levels of NF-κB p65 and T-lymphocyte subsets were measured. Positive RKIP expression was higher in gastric cardia adenocarcinoma tissues than in precancerous tissues. The serum level of total NF-κB p65 was higher in patients with gastric cardia adenocarcinoma than in healthy controls. Levels of NF-κB p65 did not correlate with positive and negative expression of RKIP, but were higher in patients with lymph node metastasis than in those without it. The cellular immune function of the gastric cardia adenocarcinoma group was lower than in normal controls, particularly in cases with negative RKIP expression. RKIP is downregulated in gastric cardia adenocarcinoma tissues, which is related to the occurrence, progression, invasion, and metastasis of tumors. The possible mechanism for this may be the inhibition of NF-κB activity and cellular immune function, which allows for the escape of tumor cells from immune surveillance.

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Correlation among RKIP expression, NF-κB p65 levels, and T-lymphocyte subsets in gastric cardia adenocarcinoma.

Objective To observe the expression changes of Raf kinase inhibitor protein( RKIP) and epithelial cadherin( E-cadherin) in gastric cardia adenocarcinoma( GCA) and to explore their correlation. Methods The expression of RKIP and E-cadherin were detected in 160 cases of GCA tissues and 160 cases of adjacent cancerous tissues by immunohistochemical SP method. We analyzed the relationship between their expression and clinical pathological parameters as well as their correlations. Results The expressions of RKIP and E-cadherin in GCA tissues were lower than that in the adjacent cancerous tissues( all P 0. 05). The expression of RKIP and E-cadherin in highly,moderately,poorly differentiated GCA was significantly different( P 0. 01) and the expression in GCA tissues with lymphatic metastasis was lower than that without lymphatic metastasis( all P 0. 05). The expression of RKIP and E-cadherin in GCA tissues were positively correlated( r =0. 388,P 0. 05). Conclusion The expression of RKIP and E-cadherin in GCA tissues is decreased and positively correlated with each other.

Wei Hon

Papers

Expression changes of Raf kinase inhibitor protein and E-cadherin in gastric cardia adenocarcinoma and their correlation