William H. Grover

TL;DR: In this paper, elastomer membrane valves and diaphragm pumps suitable for large-scale integration into glass microfluidic analysis devices are fabricated and characterized, and a three-layer valve and pump design features simple non-thermal device bonding and a hybrid glass-PDMS fluidic channel.

TL;DR: A suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control was used to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells, finding that heavier cells grew faster than lighter cells.

TL;DR: In this paper, a monolithic elastomer membrane associated with an integrated pneumatic manifold allows the placement and actuation of a variety of fluid control structures, such as structures for pumping, isolating, mixing, routing, merging, splitting, preparing, and storing volumes of fluid.

TL;DR: The ability to measure single-cell density will provide valuable insights into cell state for a wide range of biological processes and is demonstrated with four examples: identifying Plasmodium falciparum malaria-infected erythrocytes in a culture, distinguishing transfused blood cells from a patient’s own blood, identifying irreversibly sickled cells in a sickle cell patient, and identifying leukemia cells in the early stages of responding to a drug treatment.

TL;DR: Novel latching microfluidic valve structures are developed, characterized, and controlled independently using an on-chip pneumatic demultiplexer that can reduce the size, power consumption, and cost of microfluidity analysis devices by decreasing the number of off-chip controllers.