William Scher

TL;DR: This action of dimethyl sulfoxide, which was reversible, may represent the derepression of leukemic cells to permit their maturation in cloned line of murine virus-induced erythroleukemia.

TL;DR: In this paper, the effect of DMSO on leukemic cells was investigated and it was shown that 2% methyl sulfoxide (DMS0) increased the synthesis of hematopoietic cells.

TL;DR: Tryptic peptides characteristic of mouse hemoglobin α- and diffuse-type β-chains were isolated from dimethylsulfoxide-treated mouse leukemia virus-infected cell cultures, reflecting a synthetic capacity that is probably sufficient for 32P labeling of hemoglobin messenger RNAs (mRNA) in quantities commensurate with structural characterization of mRNAs by radioautographic methods.

TL;DR: 5‐Bromo‐2′‐deoxyuridine (BUdR) (10−5M) inhibits both DMF‐ and DMSO‐stimulated differentiation, and must be present during the first two days of growth, during which time DNA synthesis is maximal.

TL;DR: Hemoglobin synthesis in murine Friend virus-induced leukemic cells established in tissue culture has been demonstrated and the hemoglobin present in these cells was identified by its histochemical staining properties, peroxidatic activity, incorporation of 59 Fe, absorption spectrum, and characteristic migration pattern during discontinuous electrophoresis on polyacrylamide gels.