William W. Metcalf

TL;DR: A new genetic technique for constructing mutants of Methanosarcina acetivorans C2A by using hpt as a counterselectable marker was developed and the markerless exchange method was used to recombine a series of uidA reporter gene fusions into the M. acetIVorans proC locus.

TL;DR: The data presented here show that the ability to synthesize diverse phosphonates is both widespread and relatively common among microbes, supporting the idea that these molecules play a significant role in the biology of producing organisms and suggesting that the role of phosphonate molecules in the biosphere may be more important than is often recognized.

TL;DR: Surprisingly, BAP catalyzes the oxidation of Pt to phosphate and molecular H2 and is a unique Pt-dependent, H2-evolving hydrogenase, which is unprecedented in both P and H biochemistry.

TL;DR: The results indicate that the PhnC-to-phnP gene cluster is an operon of 14 genes, and the phnC promoter is the sole psi promoter; three gene products probably constitute a binding protein-dependent Pn transporter; seven gene products are required for catalysis and are likely to constitute a membrane-associated carbon-phosphorus (C-P) lyase; and two gene products may be accessory proteins for the C-P lyase.