W
William W. Metcalf
Researcher at University of Illinois at Urbana–Champaign
Publications - 167
Citations - 11242
William W. Metcalf is an academic researcher from University of Illinois at Urbana–Champaign. The author has contributed to research in topics: Methanosarcina acetivorans & Methanosarcina. The author has an hindex of 57, co-authored 156 publications receiving 9737 citations. Previous affiliations of William W. Metcalf include Purdue University & Howard Hughes Medical Institute.
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A computational framework for systematic exploration of biosynthetic diversity from large-scale genomic data
Jorge C. Navarro-Muñoz,Nelly Selem-Mojica,Michael W. Mullowney,Satria A. Kautsar,James H. Tryon,Elizabeth I. Parkinson,Emmanuel L. C. de los Santos,Marley Yeong,Pablo Cruz-Morales,Sahar Abubucker,Arne Roeters,Wouter Lokhorst,Antonio Fernandez-Guerra,Luciana Teresa Dias Cappelini,Regan J. Thomson,William W. Metcalf,Neil L. Kelleher,Francisco Barona-Gómez,Marnix H. Medema +18 more
TL;DR: The discovery potential of the BiG-SCAPE platform is demonstrated by using CORASON to comprehensively map the phylogenetic diversity of the large detoxin/rimosamide gene cluster clan, prioritizing three new detoxin families for subsequent characterization of six new analogs using isotopic labeling and analysis of tandem mass spectrometric data.
Journal ArticleDOI
Directed Mutagenesis and Plasmid-Based Complementation in the Methanogenic Archaeon Methanosarcina acetivorans C2A Demonstrated by Genetic Analysis of Proline Biosynthesis
TL;DR: The method employs homologous recombination-mediated gene replacement and was used to construct a variety of proline auxotrophs with mutations in the proABC locus, the first use of directed mutagenesis in Methanosarcina acetivorans C2A.
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The 2-Aminoethylphosphonate-Specific Transaminase of the 2-Aminoethylphosphonate Degradation Pathway
Alexander D. Kim,Angela Baker,Debra Dunaway-Mariano,William W. Metcalf,Barry L. Wanner,Brian M. Martin +5 more
TL;DR: Substrate specificity tests revealed a high degree of discrimination, indicating a singular physiological role for the transaminase in AEP degradation, and site-directed mutagenesis demonstrated the importance of three selected residues (Asp168, Lys194, and Arg340) in A EPT catalysis.
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Development of genetic methods and construction of a chromosomal glnK1 mutant in Methanosarcina mazei strain Gö1.
Claudia Ehlers,Claudia Ehlers,Katrin Weidenbach,Katrin Weidenbach,Katharina Veit,Katharina Veit,Uwe Deppenmeier,William W. Metcalf,Ruth A. Schmitz,Ruth A. Schmitz +9 more
TL;DR: It is proposed that the archaeal GlnK1 protein is not directly involved in the transcriptional regulation of genes involved in nitrogen metabolism, but rather affects their protein products directly.
Journal ArticleDOI
Energy Conservation and Hydrogenase Function in Methanogenic Archaea, in Particular the Genus Methanosarcina.
TL;DR: This review discusses recent insight into methanogen metabolism and energy conservation mechanisms with a particular focus on the genus Methanosarcina.