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Woong Y. Hwang
Researcher at Yale University
Publications - 9
Citations - 3723
Woong Y. Hwang is an academic researcher from Yale University. The author has contributed to research in topics: Transcription activator-like effector nuclease & CRISPR. The author has an hindex of 8, co-authored 9 publications receiving 3352 citations. Previous affiliations of Woong Y. Hwang include Harvard University.
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Journal ArticleDOI
Efficient genome editing in zebrafish using a CRISPR-Cas system
Woong Y. Hwang,Yanfang Fu,Deepak Reyon,Morgan L. Maeder,Shengdar Q. Tsai,Jeffry D. Sander,Randall T. Peterson,Randall T. Peterson,Jing-Ruey J. Yeh,J. Keith Joung +9 more
TL;DR: It is shown that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.
Journal ArticleDOI
Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas System
Woong Y. Hwang,Yanfang Fu,Deepak Reyon,Morgan L. Maeder,Prakriti Kaini,Jeffry D. Sander,J. Keith Joung,Randall T. Peterson,Randall T. Peterson,Jing-Ruey J. Yeh +9 more
TL;DR: It is demonstrated that RGN-induced mutations are heritable, with efficiencies of germline transmission reaching as high as 100% and the utility of the CRISPR-Cas system in the zebrafish expands beyond somatic indel formation to heritable and precise genome modifications.
Journal ArticleDOI
Highly efficient generation of heritable zebrafish gene mutations using homo- and heterodimeric TALENs
Lindsay Cade,Deepak Reyon,Woong Y. Hwang,Shengdar Q. Tsai,Samir Patel,Cyd Khayter,J. Keith Joung,Jeffry D. Sander,Randall T. Peterson,Jing-Ruey J. Yeh +9 more
TL;DR: The results suggest that construction of one to two heterodimeric TALEN pairs for any given gene will, in most cases, enable researchers to rapidly generate knockout zebrafish.
Journal ArticleDOI
RAPGEF5 Regulates Nuclear Translocation of β-Catenin
John N. Griffin,John N. Griffin,Florencia del Viso,Anna R. Duncan,Andrew Robson,Woong Y. Hwang,Saurabh S. Kulkarni,Karen J. Liu,Mustafa K. Khokha +8 more
TL;DR: A model whereby RAPGEF5 activates the nuclear GTPases, Rap1a/b, to facilitate the nuclear transport of β-catenin, defining a parallel nuclear transport pathway to Ran is proposed and suggested new targets for modulating Wnt signaling in disease states are suggested.
Journal ArticleDOI
Methods for targeted mutagenesis in zebrafish using TALENs.
TL;DR: Method for constructing TALEN vectors that have been shown to achieve high success rates and mutation efficiencies in zebrafish are described and simple techniques and protocols are discussed that can be used to detect TALen-induced mutations at almost any genomic locus.