M
Morgan L. Maeder
Researcher at Harvard University
Publications - 74
Citations - 16928
Morgan L. Maeder is an academic researcher from Harvard University. The author has contributed to research in topics: CRISPR & Zinc finger. The author has an hindex of 36, co-authored 74 publications receiving 15108 citations.
Papers
More filters
Journal ArticleDOI
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells.
Yanfang Fu,Jennifer A Foden,Cyd Khayter,Morgan L. Maeder,Deepak Reyon,J. Keith Joung,Jeffry D. Sander +6 more
TL;DR: It is found that single and double mismatches are tolerated to varying degrees depending on their position along the guide RNA (gRNA)-DNA interface, and off-target cleavage of CRISPR-associated (Cas)9-based RGNs is characterized.
Journal ArticleDOI
Efficient genome editing in zebrafish using a CRISPR-Cas system
Woong Y. Hwang,Yanfang Fu,Deepak Reyon,Morgan L. Maeder,Shengdar Q. Tsai,Jeffry D. Sander,Randall T. Peterson,Randall T. Peterson,Jing-Ruey J. Yeh,J. Keith Joung +9 more
TL;DR: It is shown that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.
Journal ArticleDOI
Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and in vivo
John A. Zuris,David B. Thompson,Yilai Shu,John Paul Guilinger,Jeffrey L. Bessen,Johnny Hao Hu,Morgan L. Maeder,J. Keith Joung,Zheng-Yi Chen,David R. Liu +9 more
TL;DR: It is reported that common cationic lipid nucleic acid transfection reagents can potently deliver proteins that are fused to negatively supercharged proteins, that contain natural anionic domains or that natively bind to anionic nucleic acids.
Journal ArticleDOI
CRISPR RNA–guided activation of endogenous human genes
TL;DR: This proof-of-principle work shows that clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems can target heterologous effector domains to endogenous sites in human cells.
Journal ArticleDOI
Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification.
Morgan L. Maeder,Stacey Thibodeau-Beganny,Anna Osiak,David A. Wright,Reshma M. Anthony,Magdalena Eichtinger,Tao Jiang,Jonathan E. Foley,Ronnie J. Winfrey,Jeffrey A. Townsend,Erica Unger-Wallace,Jeffry D. Sander,Felix Müller-Lerch,Fengli Fu,Joseph Pearlberg,Carl Göbel,Justin P. Dassie,Shondra M. Pruett-Miller,Matthew H. Porteus,Dennis C. Sgroi,A. John Iafrate,Drena Dobbs,Paul B. McCray,Toni Cathomen,Daniel F. Voytas,J. Keith Joung +25 more
TL;DR: OP (Oligomerized Pool ENgineering), a rapid, publicly available strategy for constructing multifinger arrays, which is more effective than the previously published modular assembly method and provides an "open-source" method for rapidly engineering highly active zinc-finger arrays.