Xi Ren

TL;DR: SDS-based perfusion decellularization can be applied to whole porcine and human lungs to generate biocompatible organ scaffolds with preserved ECM composition and architecture.

TL;DR: Combining the unique structural and biochemical properties of native acellular scaffolds with subsequent recellularization techniques offers a novel platform for organ engineering and regeneration, for experimentation ex vivo and potential clinical application in vivo.

TL;DR: The regeneration of functional pulmonary vasculature is reported by repopulating the vascular compartment of decellularized rat and human lung scaffolds with human cells, including endothelial and perivascular cells derived from induced pluripotent stem cells.

TL;DR: An enhancer trap transgenic zebrafish line, ETvmat2:GFP, in which most monoaminergic neurons are labeled by green fluorescent protein (GFP) during embryonic development is described, and it is shown that the effects of hedgehog signaling pathway inhibition on the development of monoaminationergic neurons can be easily visualized in individual living ETvMat2: GFP embryos.

TL;DR: Decellularized lung matrix supports the culture and lineage commitment of human iPSC-derived lung progenitor cells and may enable further in vivo graft maturation and enhance early lung fate.