Xiaowei Zhang

TL;DR: The benefit, both in terms of extended cell culture viability and increased product yield, of mild hypothermic cultivation conditions for transient gene expression in Chinese hamster ovary (CHO) cells is confirmed.

TL;DR: The feasibility of establishing a robust, standardized, and transferable technical platform for mammalian cell culture based on orbital shaking and disposable materials has been established with further optimizations and studies ongoing.

TL;DR: In this article, the oxygen transfer capacities of cylindrical containers with nominal volumes from 50 mL to 2000 L were determined, and the operating parameters influencing oxygen transfer were investigated; the results demonstrated the feasibility of orbital shaking technology for the cultivation of mammalian cells at scales up to 1000 L.

TL;DR: Cultivations of CHO cells in a shaken 55-L helical track bioreactor resulted in improved cell growth profiles compared to control cultures in standard systems, demonstrating the possibility of using these new bioreactors at scales of 1000 L or more.

TL;DR: An overview of the engineering characteristics when these new principles are applied in bioreactors are presented, and case studies covering scales of operation from milliliters to 1000 liters are presented.