This review highlights various methods that can be used for a sensitive detection of nucleic acids without using thermal cycling procedures, as is done in PCR or LCR. Topics included are nucleic acid sequence-based amplification (NASBA), strand displacement amplification (SDA), loop-mediated amplification (LAMP), Invader assay, rolling circle amplification (RCA), signal mediated amplification of RNA technology (SMART), helicase-dependent amplification (HDA), recombinase polymerase amplification (RPA), nicking endonuclease signal amplification (NESA) and nicking endonuclease assisted nanoparticle activation (NENNA), exonuclease-aided target recycling, Junction or Y-probes, split DNAZyme and deoxyribozyme amplification strategies, template-directed chemical reactions that lead to amplified signals, non-covalent DNA catalytic reactions, hybridization chain reactions (HCR) and detection via the self-assembly of DNA probes to give supramolecular structures. The majority of these isothermal amplification methods can detect DNA or RNA in complex biological matrices and have great potential for use at point-of-care.

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Isothermal amplified detection of DNA and RNA

Recent advances in biosensor development for the detection of cancer biomarkers

microRNA biosensors: Opportunities and challenges among conventional and commercially available techniques.

Growing demands for ultrasensitive biosensing have led to the development of numerous signal-amplification strategies. Based on their unique properties (i.e., electro-conductivity, bio-compatibility and ease of synthesis), various iron magnetic nanoparticles (MNPs) have proved to be an excellent nanomaterial for applications in electrochemical biosensing. This review shows how iron MNPs have made significant contributions in the development of electrochemical nanobiosensors, including immuno-, enzyme, DNA and aptamer types. More importantly, we discuss in detail different aspects of the electrochemical biosensors (e.g., modes of magnetic particles, detection techniques, analytes and the corresponding sensitivity and sample matrix, and several prominent characteristics). Accordingly, we discuss research opportunities and future development trends in these areas.

Iron and iron-oxide magnetic nanoparticles as signal-amplification elements in electrochemical biosensing

/pdf/emerging-biosensing-approaches-for-microrna-analysis-qz5p3r3icf.pdf

Emerging Biosensing Approaches for microRNA Analysis

A novel electrically magnetic-controllable electrochemical biosensor for the ultra sensitive and specific detection of attomolar level oral cancer-related microRNA

XiaoYan Wu

Papers

A novel electrically magnetic-controllable electrochemical biosensor for the ultra sensitive and specific detection of attomolar level oral cancer-related microRNA

A signal-on electrochemiluminescence aptamer biosensor for the detection of ultratrace thrombin based on junction-probe.

Electrochemical genotyping and detection of single-nucleotide polymorphisms based on junction-probe containing 2′-deoxyinosine

Ultra-sensitive electrochemical detection of single nucleotide polymorphisms based on an electrically controllable magnetic gold electrode