Xingyong Wu

TL;DR: RNAscope is described, a novel RNA ISH technology with a unique probe design strategy that allows simultaneous signal amplification and background suppression to achieve single-molecule visualization while preserving tissue morphology and may enable rapid development of RNAISH-based molecular diagnostic assays.

TL;DR: The data presented in this study demonstrate that the automated RNAscope technology is a high performance RNA ISH assay with broad applicability in biomarker research and diagnostic assay development.

TL;DR: In this paper, the AQUA method of quantitative immunofluorescence (QIF) was used for measuring mRNA in situ using ESR1 (the estrogen receptor alpha gene) in breast cancer to determine its predictive value compared to ESRα (ER) protein.

TL;DR: The authors present BaseScope, a mutation-specific RNA in situ hybridization assay that targets common point mutations in the BRAF, KRAS and PIK3CA oncogenes in archival colorectal cancer samples to precisely map the spatial and morphological context of mutant subclones.

TL;DR: The dual discernment of diffuse and/or dot-like signals together with the assay’s high sensitivity for HPV support the use of HPV E6/E7 RNA CISH as an adjunct test for deciding lesion grade when CIN 2 grading may be beneficial (e.g. among young women) or when ‘LSIL vs. HSIL’ assignment is equivocal.