Significance Methanol-based biomanufacturing is considered a promising way to achieve carbon neutrality. However, efficient methanol biotransformation toward chemical production is challenging. To address this challenge, we enhanced the precursor supply and coenzyme regeneration in Pichia pastoris, combined with reinforcing methanol assimilation, which resulted in significant improvements in fatty acid production. Furthermore, we offered an effective approach for rapidly constructing versatile cell factories that share common precursors. This study represents an important step forward in the rewiring of P. pastoris as industrial microbial cell factories for chemical production.

Methanol biotransformation toward high-level production of fatty acid derivatives by engineering the industrial yeast Pichia pastoris

A navigation guide of synthetic biology tools for Pseudomonas putida.

As a green and renewable resource, marine polysaccharides have inherent nontoxicity, good biocompatibility, biodegradability, and environmental friendliness. Compared to land-based polysaccharides, marine polysaccharides acquire an absolute advantage in the process of diversity, extraction and purification. In recent years, some marine polysaccharides (such as alginate and chitosan) have been successfully converted into commercial products and widely used in clinical wound repair. Marine polysaccharide-based dressings can be prepared into multiple types of products (hydrogels, films, foam, or sponge) according to different application scenarios, providing excellent conditions for wound healing. These types of dressings can block bacteria, maintain the wound microenvironment, and promote wound healing. The development and utilization of marine polysaccharides are also expected to solve ecological problems such as green algae tide and marine biological invasions. This review presents the advances in marine polysaccharide-based wound dressings in the past three years. The direction of improvements in wound dressings based on marine polysaccharides in the future is also prospected.

Marine polysaccharides: green and recyclable resources as wound dressings

Biosynthesis of non-animal chondroitin sulfate from methanol using genetically engineered Pichia pastoris

Combinatorial metabolic engineering of Escherichia coli for de novo production of 2'-fucosyllactose.

Many genetic tools for gene regulation have been developed during the past decades Some of them edit genomic DNA, such as nucleotides deletions and insertions, while the others interfere with the gene transcriptions or messenger RNA translation Here, we report a posttranscriptional regulation tool which is termed "Modulation via the small RNA (sRNA)-dependent operation system: MS-DOS" by engineering the type I toxin-antitoxin system in Bacillus subtilis MS-DOS depends simply on insertion of an operation region (OPR; partial toxin-encoding region) downstream of a genomic open reading frame of interest and overexpression of the coupling antitoxin sRNA from a plasmid Pairing between the OPR and the sRNA will trigger the RNAse degradation of the transcripts of selected genes MS-DOS allows for the quantitative, specific, and reversible knockdown of single or multiple genomic genes in B subtilis We also showed that the truncated antitoxin SR4 with 53 nt length is sufficient to repress gene expression Superior to other existing RNA based interfering systems, MS-DOS allows simultaneous knockdown of multiple genes with effortless expression of a single antitoxin RNA This sRNA-guided repression system will further enrich the gene regulation tools and expand the gene regulation flexibility

Xuerong Jin

Papers

Biosynthesis of non-animal chondroitin sulfate from methanol using genetically engineered Pichia pastoris

A new sRNA‐mediated posttranscriptional regulation system for Bacillus subtilis

Closed-Loop System Driven by ADP Phosphorylation from Pyrophosphate Affords Equimolar Transformation of ATP to 3′-Phosphoadenosine-5′-phosphosulfate

Food-grade expression of an iron-containing acid urease in Bacillus subtilis

Optimizing the sulfation-modification system for scale preparation of chondroitin sulfate A.