Journal ArticleDOI
Biosynthesis of non-animal chondroitin sulfate from methanol using genetically engineered Pichia pastoris
Xuerong Jin,Weijiao Zhang,Yang Wang,Jingyu Sheng,Ruirui Xu,Jianghua Li,Guocheng Du,Zhen Kang +7 more
TLDR
Establishment of a sustainable non-animal biosynthesis of chondroitin sulfate from methanol using genetically engineered Pichia pastoris.About:
This article is published in Green Chemistry.The article was published on 2021-06-21. It has received 24 citations till now. The article focuses on the topics: Chondroitin & Pichia pastoris.read more
Citations
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Methanol biotransformation toward high-level production of fatty acid derivatives by engineering the industrial yeast Pichia pastoris
TL;DR: It is demonstrated that global metabolic rewiring released the great potential of P. pastoris for methanol biotransformation toward chemical overproduction.
Journal ArticleDOI
Synthesis of bioengineered heparin by recombinant yeast Pichia pastoris
Yongling Zhang,Yang Wang,Zheng-Chuan Zhou,Pei-Yu Wang,Xintong Xi,Shanwen Hu,Ruirui Xu,Guocheng Du,Jianghua Li,Jian-Sheng Chen,Zhen-Yan Kang +10 more
TL;DR: Production of bioengineered heparin with recombinant yeast cells for use in drug discovery and development of new drugs for wound healing and wound healing properties.
Journal ArticleDOI
Metabolic engineering of methylotrophic Pichia pastoris for the production of β-alanine
TL;DR: In this article, the feasibility of β-alanine production from methanol using Pichia pastoris (Komagataella phaffii) as a methylotrophic cell factory was demonstrated.
Journal ArticleDOI
Heterologous production of chondroitin
TL;DR: In this article , the recent advances on enzymatic and microbial strategies to produce non-animal chondroitin are reviewed and challenges and prospects for future research are also discussed.
Journal ArticleDOI
High-level de novo biosynthesis of cordycepin by systems metabolic engineering in Yarrowia lipolytica.
Xiuting Duan,Yun Tian,Ze Song,Li-Ping Song,Wenbo Lin,Chong Wang,Hui Yang,Xiangyang Lu,Xiao-Jun Ji,Huhu Liu +9 more
TL;DR: In this paper , a promising cell factory for high-level cordycepin production was constructed in Yarrowia lipolytica, which achieved a yield of 3588.59 mg/L from glucose and 76.46 mg/g total sugar.
References
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Journal ArticleDOI
Fiji: an open-source platform for biological-image analysis
Johannes Schindelin,Ignacio Arganda-Carreras,Erwin Frise,Verena Kaynig,Mark Longair,Tobias Pietzsch,Stephan Preibisch,Curtis Rueden,Stephan Saalfeld,Benjamin Schmid,Jean-Yves Tinevez,Daniel J. White,Volker Hartenstein,Kevin W. Eliceiri,Pavel Tomancak,Albert Cardona +15 more
TL;DR: Fiji is a distribution of the popular open-source software ImageJ focused on biological-image analysis that facilitates the transformation of new algorithms into ImageJ plugins that can be shared with end users through an integrated update system.
Journal ArticleDOI
A modified uronic acid carbazole reaction
T. Bitter,Helen Muir +1 more
TL;DR: It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.
Journal ArticleDOI
Biological Roles of Glycans
TL;DR: It is time for the diverse functional roles of glycans to be fully incorporated into the mainstream of biological sciences, as they are no different from other major macromolecular building blocks of life, simply more rapidly evolving and complex.
Journal ArticleDOI
Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector.
Andrea L. Szymczak,Andrea L. Szymczak,Creg J. Workman,Yao Wang,Kate M. Vignali,Smaroula Dilioglou,Elio F. Vanin,Elio F. Vanin,Dario A. A. Vignali,Dario A. A. Vignali +9 more
TL;DR: It is shown that a single 2A peptide–linked retroviral vector can be used to generate all four CD3 proteins, and restore T-cell development and function in CD3-deficient mice.
Journal ArticleDOI
Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes
TL;DR: The observed asymmetry suggests that purines in positions -3 and +4 might facilitate recognition of the AUG condon during formation of initiation complexes and a modified version of the scanning mechanism is proposed.