Y
Yasuki Fukuda
Researcher at Kyoto University
Publications - 18
Citations - 256
Yasuki Fukuda is an academic researcher from Kyoto University. The author has contributed to research in topics: Methylglyoxal & Enzyme. The author has an hindex of 7, co-authored 18 publications receiving 249 citations.
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Journal ArticleDOI
Metabolism of 2-oxoaldehyde in yeasts. Purification and characterization of NADPH-dependent methylglyoxal-reducing enzyme from Saccharomyces cerevisiae.
Kousaku Murata,Yasuki Fukuda,Makoto Simosaka,Kunihiko Watanabe,Toshihiko Saikusa,Akira Kimura +5 more
TL;DR: An enzyme catalyzing the reduction of methylglyoxal was isolated from Saccharomyces cerevisiae and its enzymatic properties were analyzed and it was tentatively designated the enzyme methyl glyoxal reductase.
Journal ArticleDOI
Characterization of methylglyoxal synthase in Saccharomyces cerevisiae
Kousaku Murata,Yasuki Fukuda,Kunihiko Watanabe,Toshihiko Saikusa,Makoto Shimosaka,Akira Kimura +5 more
TL;DR: Methylglyoxal synthase in Saccharomyces cerevisiae was purified approximately 300 folds from cell extracts with 20% of activity yield and during purification procedures, polymorphic behaviours of the enzyme were observed.
Journal ArticleDOI
Metabolism of 2-oxoaldehydes in yeasts
Yoshiharu Inoue,Kunihiko Watanabe,Makoto Shimosaka,Toshihiko Saikusa,Yasuki Fukuda,Kousaku Murata,Akira Kimura +6 more
TL;DR: The result indicated the possible role of glycolytic bypath as a detoxification system of methylglyoxal formed from l-threonine catabolism.
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Metabolism of .ALPHA.-ketoaldehydes in yeasts: Purification and characterization of glyoxalase II from Saccharomyces cerevisiae.
Kousaku Murata,Yoshiharu Inoue,Kunihiko Watanabe,Yasuki Fukuda,Toshihiko Sadcusa,Makoto Shimosaka,Akira Kimura +6 more
TL;DR: Hemimercaptal, a non-enzymatic condensation product of methylglyoxal and glutathione, strongly inhibited the activity of the enzyme, and glyoxalase II was purified approximately 100 fold from a cell extract with a 20% activity yield.
Journal ArticleDOI
Metabolism of α-ketoaldehydes in yeasts: Inducible formation of methyglyoxal reductase and its relation to growth arrest of Saccharomyces cerevisiae
Kousaku Murata,Yoshiharu Inoue,Toshihiko Saikusa,Kunihiko Watanabe,Yasuki Fukuda,Shimosoka Makoto,Akira Kimura +6 more
TL;DR: Low intracellular S -lactoylglutathione concentration was postulated to account for the observed growth arrest of Saccharomyces cerevisiae cells with hybrid plasmid pYMG14 carrying a gene for NADPH-dependent methylglyoxal reductase of the yeast.