Z
Zach Klapholz O’Brown
Researcher at Boston Children's Hospital
Publications - 6
Citations - 286
Zach Klapholz O’Brown is an academic researcher from Boston Children's Hospital. The author has contributed to research in topics: DNA & Gene. The author has an hindex of 4, co-authored 4 publications receiving 153 citations. Previous affiliations of Zach Klapholz O’Brown include Harvard University.
Papers
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Journal ArticleDOI
Sources of artifact in measurements of 6mA and 4mC abundance in eukaryotic genomic DNA.
Zach Klapholz O’Brown,Zach Klapholz O’Brown,Konstantinos Boulias,Konstantinos Boulias,Jie Wang,Simon Yuan Wang,Simon Yuan Wang,Natasha M O'Brown,Ziyang Hao,Hiroki Shibuya,Hiroki Shibuya,Hiroki Shibuya,Paul-Enguerrand Fady,Yang Shi,Yang Shi,Chuan He,Sean G. Megason,Tao Liu,Eric L. Greer,Eric L. Greer +19 more
TL;DR: It is demonstrated that 6mA and 4mC are rarer in metazoa than previously reported, and highlight the importance of careful sample preparation and measurement, and need for more accurate sequencing techniques.
Book ChapterDOI
N6-Methyladenine: A Conserved and Dynamic DNA Mark.
TL;DR: This chapter will focus on methylation of the sixth position on adenines (6mA), as this modification has been poorly characterized in recently evolved eukaryotes, but shows promise as a new conserved layer of epigenetic regulation.
Journal ArticleDOI
N6-adenosine methylation of ribosomal RNA affects lipid oxidation and stress resistance
Noa Liberman,Noa Liberman,Zach Klapholz O’Brown,Zach Klapholz O’Brown,Andrew S. Earl,Andrew S. Earl,Konstantinos Boulias,Konstantinos Boulias,Maxim V. Gerashchenko,Simon Yuan Wang,Simon Yuan Wang,Colette Fritsche,Paul-Enguerrand Fady,Anna Dong,Vadim N. Gladyshev,Eric L. Greer,Eric L. Greer +16 more
TL;DR: It is suggested that ribosome methylation can facilitate selective translation, providing another layer of regulation of the stress response, and blocking this pathway increases stress resistance in C. elegans.
Journal ArticleDOI
The demethylase NMAD-1 regulates DNA replication and repair in the Caenorhabditis elegans germline.
Simon Yuan Wang,Simon Yuan Wang,Hui Mao,Hui Mao,Hiroki Shibuya,Hiroki Shibuya,Satoru Uzawa,Zach Klapholz O’Brown,Zach Klapholz O’Brown,Sage Wesenberg,Nara Shin,Takamune T. Saito,Jinmin Gao,Barbara J Meyer,Monica P. Colaiácovo,Eric L. Greer,Eric L. Greer +16 more
TL;DR: Genetic evidence for a critical role for the putative DNA demethylase NMAD-1 in regulating meiosis in C. elegans is reported and immunoprecipitation and mass spectrometry results suggest that NMad-1 functions at DNA replication sites to regulate DNA replication and repair during meiosis.
Journal ArticleDOI
Hypoxia induces transgenerational epigenetic inheritance of small RNAs
Simon Yuan Wang,Kathleen Kim,Zach Klapholz O’Brown,Aileen Levan,Anne E. Dodson,Scott Kennedy,Chaim Chernoff,Eric L. Greer +7 more
TL;DR: This article showed that hypoxia exposure in ancestors can elicit phenotypic consequences in normoxia-reared descendants in the nematode Caenorhabditis elegans.