Amrita Vishwa Vidyapeetham

About: Amrita Vishwa Vidyapeetham is a education organization based out in Coimbatore, India. It is known for research contribution in the topics: Computer science & Deep learning. The organization has 14354 authors who have published 11082 publications receiving 76150 citations. The organization is also known as: Amrita University & Amrita Vishwa Vidyāpeetham.

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

Abstract: In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.

Deep Learning Approach for Intelligent Intrusion Detection System

Abstract: Machine learning techniques are being widely used to develop an intrusion detection system (IDS) for detecting and classifying cyberattacks at the network-level and the host-level in a timely and automatic manner. However, many challenges arise since malicious attacks are continually changing and are occurring in very large volumes requiring a scalable solution. There are different malware datasets available publicly for further research by cyber security community. However, no existing study has shown the detailed analysis of the performance of various machine learning algorithms on various publicly available datasets. Due to the dynamic nature of malware with continuously changing attacking methods, the malware datasets available publicly are to be updated systematically and benchmarked. In this paper, a deep neural network (DNN), a type of deep learning model, is explored to develop a flexible and effective IDS to detect and classify unforeseen and unpredictable cyberattacks. The continuous change in network behavior and rapid evolution of attacks makes it necessary to evaluate various datasets which are generated over the years through static and dynamic approaches. This type of study facilitates to identify the best algorithm which can effectively work in detecting future cyberattacks. A comprehensive evaluation of experiments of DNNs and other classical machine learning classifiers are shown on various publicly available benchmark malware datasets. The optimal network parameters and network topologies for DNNs are chosen through the following hyperparameter selection methods with KDDCup 99 dataset. All the experiments of DNNs are run till 1,000 epochs with the learning rate varying in the range [0.01-0.5]. The DNN model which performed well on KDDCup 99 is applied on other datasets, such as NSL-KDD, UNSW-NB15, Kyoto, WSN-DS, and CICIDS 2017, to conduct the benchmark. Our DNN model learns the abstract and high-dimensional feature representation of the IDS data by passing them into many hidden layers. Through a rigorous experimental testing, it is confirmed that DNNs perform well in comparison with the classical machine learning classifiers. Finally, we propose a highly scalable and hybrid DNNs framework called scale-hybrid-IDS-AlertNet which can be used in real-time to effectively monitor the network traffic and host-level events to proactively alert possible cyberattacks.

Stock price prediction using LSTM, RNN and CNN-sliding window model

Abstract: Stock market or equity market have a profound impact in today's economy. A rise or fall in the share price has an important role in determining the investor's gain. The existing forecasting methods make use of both linear (AR, MA, ARIMA) and non-linear algorithms (ARCH, GARCH, Neural Networks), but they focus on predicting the stock index movement or price forecasting for a single company using the daily closing price. The proposed method is a model independent approach. Here we are not fitting the data to a specific model, rather we are identifying the latent dynamics existing in the data using deep learning architectures. In this work we use three different deep learning architectures for the price prediction of NSE listed companies and compares their performance. We are applying a sliding window approach for predicting future values on a short term basis. The performance of the models were quantified using percentage error.

Role of size scale of ZnO nanoparticles and microparticles on toxicity toward bacteria and osteoblast cancer cells

Abstract: The specific role of size scale, surface capping, and aspect ratio of zinc oxide (ZnO) particles on toxicity toward prokaryotic and eukaryotic cells was investigated. ZnO nano and microparticles of controlled size and morphology were synthesized by wet chemical methods. Cytotoxicity toward mammalian cells was studied using a human osteoblast cancer cell line and antibacterial activity using Gram-negative bacteria (Escherichia coli) as well as using Gram-positive bacteria (Staphylococcus aureus). Scanning electron microscopy (SEM) was conducted to characterize any visual features of the biocidal action of ZnO. We observed that antibacterial activity increased with reduction in particle size. Toxicity toward the human cancer cell line was considerably higher than previously observed by other researchers on the corresponding primary cells, suggesting selective toxicity of the ZnO to cancer cells. Surface capping was also found to profoundly influence the toxicity of ZnO nanoparticles toward the cancer cell line, with the toxicity of starch-capped ZnO being the lowest. Our results are found to be consistent with a membrane-related mechanism for nanoparticle toxicity toward microbes.