Showing papers by "Catholic University of the Sacred Heart published in 1982"
TL;DR: It is demonstrated here, using immunohistochemistry and electron immunocytochemistry, that both enteric glial cells and Schwann cells of the human gut contain densely immunoreactive S-100, and this protein can therefore be regarded as a common marker for the glial components of the enteric nervous system.
Abstract: Recently, the complex organization of the enteric nervous system and its independent role in the integrative control of digestive functions have been fully recognized. However, little is known about the non-neuronal elements present in close morphological and, presumably, functional relationship with the enteric neurones. Although ‘enteric glial cells’ in gut ganglia and Schwann cells accompanying nerve fibres throughout the gastrointestinal wall can easily be recognized by electron microscopy on the basis of their ultrastructural features1–3, the lack of a suitable common marker for the visualization of these non-neuronal components of the enteric nervous system has hampered investigation. S-100 is a Ca2+-binding protein4 originally isolated from the brain5, where it is localized mainly in glia6,7. We demonstrate here, using immunohistochemistry and electron immunocytochemistry, that both enteric glial cells and Schwann cells of the human gut contain densely immunoreactive S-100. This protein can therefore be regarded as a common marker for the glial components of the enteric nervous system.
205 citations
TL;DR: The existence of divergent axon collaterals of neurons in the deep cerebellar nuclei has been investigated in rat by means of the fluorescent retrograde double labeling technique and the results have led to the following conclusions.
Abstract: The existence of divergent axon collaterals of neurons in the deep cerebellar nuclei has been investigated in rat by means of the fluorescent retrograde double labeling technique. The results have led to the following conclusions.
A.
Many of the neurons in the lateral, the interpositus as well as the caudal half of the medial nucleus project to the diencephalon. Some of these neurons distribute divergent axon collaterals to the superior colliculus, but few neurons project only to the latter structure.
B.
Some of the deep cerebellar neurons located laterally, i.e. in the dorsomedial part of the lateral nucleus, as well as some others located medially, i. e. in the medial part of the interpositus nucleus and the adjoining part of the medial nucleus, distribute divergent axon collaterals to the diencephalon and the spinal cord.
C.
Deep cerebellar neurons located laterally: in the cell group of the dorsolateral hump (Dlh) and in the adjoining lateral part of the interpositus nucleus, as well as some others located medially, i.e. in the dorsolateral part of the medial nucleus (Mdlp), distribute divergent axon collaterals to the diencephalon and to the medulla oblongata, probably primarily its medial reticular formation. However, only few of the neurons, which distribute descending collaterals to the spinal cord or the medulla oblongata, distribute ascending collaterals to the superior colliculus.
D.
After injections in the medulla oblongata a population of small sized single labeled neurons was encountered especially in the lateral and interpositus nuclei. On the basis of other findings in rat they were assumed to represent cerebello-olivary neurons.
93 citations
TL;DR: A new glycoside, hypoxoside, was isolated from rhizome of Hypoxis obtusa, a plant used in Mozambique in native medicine for the treatment of urinary diseases as mentioned in this paper.
70 citations
TL;DR: According to the authors' experience, only in cases of hyperechoic well‐defined lesions in asymptomatic patients can a definite diagnosis of hemangioma be made.
Abstract: Twenty-one cases of hepatic hemangioma were examined by ultrasound, and three main sonographic patterns were identified. In 14 cases hyperechoic focal areas were observed, in five cases larger hemangiomas exhibited a complex pattern, and in two cases hemangiomas appeared as rounded sonolucent areas with distal enhancement mimicking cysts. Hemangiomas may have significantly variable ultrasonographic features. According to the authors' experience, only in cases of hyperechoic well-defined lesions in asymptomatic patients can a definite diagnosis of hemangioma be made.
59 citations
TL;DR: The composition of the extract of M. laevis from the Putumayo area of Colombia was studied in order to establish the active principle responsible for antitumor and anti-inflammatory properties and the presence of phenoldienones, catechin and proanthocyanidins confirm the properties of the extracts of the plant claimed by traditional medicine.
57 citations
TL;DR: The seeds of Milletia thonningii gave alpinumisoflavone, its monomethyl and dimethyl derivatives, robustic acid and a new pyrano-isof-lavone.
49 citations
TL;DR: The results indicate that serotonin can be released from central nerve endings by two mechanisms, differentially affected by the blockade of the membranes carrier system: the characteristics of the Ca(2+)-dependent release are compatible with an exocytotic mechanism, whereas the release induced by lack of Na(+) or by phenylethylamines and tryptamine appears to occur by outward transport mediated by the membrane carrier.
48 citations
TL;DR: The present findings confirm the existence of a non-dopaminergic (DA) mesencephalo-cortical pathway and describe the topographical interrelationships between its DA and the non-DA cell bodies of origin.
47 citations
TL;DR: A 56-yr old woman developed a cortical auditory disorder after two successive strokes involving the temporal lobes, which did not show a left car advantage with right ear suppression, as shown in other cases with similar disorders.
41 citations
TL;DR: A statistically significant poorer prognosis was observed in patients with left-sided tumors, with an emergency presentation and with a duration of symptoms shorter than six months, in patients operated upon for colorectal cancer.
Abstract: Four prognostic factors,i.e., site of the tumors, mode of presentation, duration of symptoms, and pathologic staging, were correlated to the results of surgical treatment,i.e., curative resectability, postoperative complications and mortality, and five-year survival, in 161 patients operated upon for colorectal cancer.
40 citations
TL;DR: The present data introduce new perspectives into the investigation of S-100 by the immunohistochemical PAP method, which found that S- 100-like immunoreactivity was only detectable in the cilia of the epidermal cells.
Abstract: The presence of an S-100-like immunoreactivity was investigated in the planarian Dugesia gonocephala By microcomplement fixation assay, measurable amounts of S-100-like immunoreactive material (011μg/mg soluble protein) were detected in planarian high-speed supernatants The index of immunological dissimilarity between ox S-100 and planarian S-100-like immunoreactive material was higher than that previously calculated between ox S-100 and all the vertebrates tested By the immunohistochemical PAP method, S-100-like immunoreactivity was only detectable in the cilia of the epidermal cells Although the biological meaning of S-100-like immunoreactivity in planarian remains to be clarified, the present data introduce new perspectives into the investigation of S-100
TL;DR: The successful transsphenoidal removal of pituitary microadenomas in 15 children is reported, in 2 of them precocious puberty was the presenting sign; in the remaining cases growth failure was the mos.
Abstract: The successful transsphenoidal removal of pituitary microadenomas in 15 children is reported. In 2 of them precocious puberty was the presenting sign; in the remaining cases growth failure was the mos
TL;DR: The secondary metabolises of Vismia guaramirangae were isolated from the bark and exudate of the leaves of the tree and the structures of a new anthraquinone, 2-isoprenylemodin, and a new lignan, 5,5′-dimethoxysesamin, were established on the basis of chemical and spectroscopic evidence as mentioned in this paper.
TL;DR: P-Hydroxybenzaldehyde is present in concentrations up to 30% in the wax of Sorghum bicolor seedlings and is highly deterrent to locusts, reducing their normal feeding by 90%.
TL;DR: It is concluded that the AP exerts an inhibitory influence on the afferent limb of the monosynaptic masseteric reflex.
TL;DR: Trichophyton mentagrophytes and Microsporum canis were isolated from apparently healthy scalps of schoolboys during an epidemiological study of dermatophyte carriers.
Abstract: Summary: Trichophyton mentagrophytes and Microsporum canis were isolated from apparently healthy scalps of schoolboys during an epidemiological study of dermatophyte carriers. Various other keratinophilic fungi were also isolated.
Zusammenfassung: Wahrend einer epidemiologischen Untersuchung auf Dermatophyten-Trager konnten Trichophyton mentagrophytes and Microsporum canis von offensichtlich gesunder Kopfhaut bei Schuljungen isoliert werden.
TL;DR: Two main phases may be distinguished in the study of the anatomical organization of the intralaminar efferents, especially of their cortical projections, mainly because of the different experimental techniques available to neuroanatomists.
Abstract: Two main phases may be distinguished in the study of the anatomical organization of the intralaminar efferents, especially of their cortical projections, mainly because of the different experimental techniques available to neuroanatomists.
TL;DR: The present results suggest that the vascular action of cadmium is due, in part at least, to the involvement of the vascular-wall and platelet prostaglandin systems.
TL;DR: In anaesthetized and paralyzed rabbits, electrical stimulation of the cervical sympathetic nerve at physiological frequencies induces in jaw muscle spindle afferents a short-latency decrease or suppression of discharge.
Abstract: In anaesthetized and paralyzed rabbits, electrical stimulation of the cervical sympathetic nerve at physiological frequencies induces in jaw muscle spindle afferents a short-latency decrease or suppression of discharge. This effect is very stereotyped in pattern and is attributed to direct sympathetic innervation of spindles. It is mediated by preganglionic S1–S2 sympathetic fiber groups. A longer-latency facilitatory effect follows, probably vasomotor in origin and mediated by S3–S4 groups. Both responses are eliminated by administration of α-adrenergic blocking agents. The latencies, patterns, thresholds, durations and reproducibility of these responses have been studied and the mechanisms possibly involved are discussed.
TL;DR: Patients with Duchenne muscular dystrophy showed no signs of altered glucose metabolism and retrospective analysis of the clinical records of a further 56 patients revealed no modification in carbohydrate metabolism.
Abstract: Compared to matched controls, 17 patients with Duchenne muscular dystrophy showed decreased insulin binding to monocytes due to decreased receptor concentration. These patients showed no signs of altered glucose metabolism and retrospective analysis of the clinical records of a further 56 such patients revealed no modification in carbohydrate metabolism. These data suggest that reduced insulin receptor number does not produce overt modifications of glucose metabolism in Duchenne muscular dystrophy.
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TL;DR: In this paper, a limited number of dramatic events in various parts of the world, whose effects were amplified through the emotional reaction of the mass media, have aroused deep concern regarding the health effects of energy production, mainly in the case of nuclear energy.
Abstract: Publisher Summary In the currently available systems of energy production, involving chemical and nuclear changes, certain products and or radiations are produced. Thus, the impact on health and quality of life from energy production is due to the nature and concentration of these products in the human environment. The damages to health are due to the pollution of air, water and soil by the by-products formed in energy production and affects the exposed population. The workers in the plants where energy is produced are subject to occupational contamination. Moreover, a limited number of dramatic events in various parts of the world, whose effects were amplified through the emotional reaction of the mass media, have aroused deep concern regarding the health effects of energy production, mainly in the case of nuclear energy. Technology is at present able to reduce the concentration of harmful substances in the human environment to acceptable levels. Energy production is the main factor not only in the development but also in the survival of mankind, represented by more than its 4 billion persons.
TL;DR: Assessment of the familial genetic pattern of serum transferrin may result to be a useful procedure for the evaluation of genetic risk of abortion and preterm delivery.
Abstract: Summary: Genetic typing of serum transferrin was performed in a group of 88 extremely premature infants (gestation age <32 wk) and in a control group of 351 full-term infants, using isoelectric focusing technique on ultrathin layer of polyacrilamide gel. A major incidence of C2 type was found among the preterm infants when compared to full-term infants X2 = 22,86, (P < 0.001).
TL;DR: An improved method for separating transferrin subtypes through the use of isoelectric focusing on ultrathin layer polyacrylamide gels is described, and the gene frequencies in three samples from Italian populations are reported.
Abstract: An improved method for separating transferrin subtypes through the use of isoelectric focusing on ultrathin layer polyacrylamide gels is described. The most considerable problems encountered in the assessment of the Tf phenotypes after pretreatment with iron donor compounds are pointed out. Useful technical devices for reducing the occurrence of artifactitious extrabands are suggested. Finally, the gene frequencies in three samples from Italian populations are reported.
TL;DR: The pathologic phenotypes appeared to decrease progressively from northern to southern Italy, and the distribution of the PiM suballeles showed considerable geographic variability, but the suballele, M2 was more frequently encountered in southern Italy.
Abstract: Genetic typing of α1AT was performed in 3751 individuals from Italian towns. The following was observed: (a) The pathologic phenotypes (SZ, MZ, ZZ) appeared to decrease progressively from northern to southern Italy; (b) the distribution of the PiM suballeles showed considerable geographic variability, but the suballele, M
2 was more frequently encountered in southern Italy; and (c) in the large cities of southern Italy, the frequency of the deficiency more closely resembled that found in northern Italy than that of the remaining populations of the south.
TL;DR: A squamous epithelioma and a uterine sarcoma were histologically confirmed in 2 of 10 albino rats fed PR toxin in a relatively short time and only an adenocarcinoma was histologically proven within a control group of 10 rats in a significantly longer time span.
Abstract: Preliminary results on the carcinogenic activity of a mycotoxin of Penicillium roqueforti (PR toxin) are reported A squamous epithelioma and a uterine sarcoma were histologically confirmed in 2 of 10 albino rats fed PR toxin in a relatively short time (449 and 551 days respectively) Only an adenocarcinoma was histologically proven within a control group of 10 rats in a significantly longer (931 days) time span
TL;DR: The results suggest that S‐100 binds to two populations of membrane sites: high‐affinity sites, which seem to be confined to neuronal membranes (synaptosomal plasma membranes and synaptic vesicles), and low-affinity Sites, which are also detected in other membranes.
Abstract: The specific interaction of S-100 protein with synaptosomal particulate fractions (SYN) was further investigated with special reference to the number of binding components and their localization in synaptosomal subfractions. Binding studies were conducted on SYN from various CNS regions, on synaptosomal subfractions from the cerebral cortex, and on cerebral cortex SYN under Various conditions. The results suggest that S-100 binds to two populations of membrane sites: high-affinity sites, which seem to be confined to neuronal membranes (synaptosomal plasma membranes and synaptic vesicles), and low-affinity sites, which are also detected in other membranes. The data are consistent with the view that the biphasic profile of S-100 binding to SYN does not result from heterogeneity of the S-100 molecule, and that the Ca2+ conformation of the protein is as important as the proper conformation of the binding site for full expression of high-affinity binding.
TL;DR: The retrograde fluorescent tracing technique was combined with the di-isopropylfluorophosphate (DFP) histochemical procedure for acetylcholinesterase (AChE) to study the efferent connections of AChE-containing neurons in the central nervous system.
TL;DR: In this article, the first report on the application of 13C NMR spectroscopy to ring C of ferruginins is presented, where a keto-enolic tautomerism is demonstrated.
TL;DR: The experience on prenatal chromosome diagnosis of ten Italian centers participating in a collaborative study on 4952 diagnoses performed from 1972 to 1980 is reported and the frequencies and types of chromosome anomalies are reported.
Abstract: The development of prenatal diagnosis in Italy was made difficult by the restrictions of the old abortion law and only in recent years has a consistent number of cases been investigated. We report the experience on prenatal chromosome diagnosis of ten Italian centers participating in a collaborative study on 4952 diagnoses performed from 1972 to 1980. The main indication groups were: advanced maternal age (2882 cases), previous child with chromosome anomaly from parents with normal karyotype (847 cases), and chromosome anomaly in one parent (97 cases). The other indications for amniocentesis, including cases without a cytogenetic risk, have been assembled into a “miscellaneous” group (1126 cases). We found 125 abnormal fetal karyotypes (2.5%) of which 89 were unbalanced (1.8%). The frequencies and types of chromosome anomalies are reported in detail for each indication group and are compared with the corresponding ones from the European Munich Conference. The great majority of these Italian data were not included in the Munich report.
TL;DR: The case of a woman with progressive systemic sclerosis (PSS) in whom the usual symptoms were preceded by a rapidly progressive peripheral neuropathy has been described in this paper, where an ultrastructural study of an affected peripheral nerve and muscle was performed.
Abstract: We present the case of a woman with progressive systemic sclerosis (PSS) in whom the usual symptoms were preceded by a rapidly progressive peripheral neuropathy. Few cases of peripheral nerve involvement have been described. For the first time we report an ultrastructural study of an affected peripheral nerve and muscle. In the sural nerve we found an almost complete loss of myelinated fibers. Schwann cells showed an abnormal hyperplasia of their basal membranes and structural signs of denervation. Spindle-shaped banded structures were seen in the cytoplasm of Schwann cells and in the endoneurium. On the basis of these ultrastructural data some hypotheses on the pathogenetic mechanism of this neuropathy are discussed.