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Showing papers by "Chiron Corporation published in 1982"

Journal Article•DOI•
Synthesis and assembly of hepatitis B virus surface antigen particles in yeast

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Pablo Valenzuela1, Pablo Valenzuela2, Angelica Medina1, William J. Rutter1, Gustav Ammerer3, Benjamin D. Hall3 •
University of California, San Francisco1, Chiron Corporation2, University of Washington3
22 Jul 1982-Nature
TL;DR: The surface antigen of hepatitis B virus has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5′-flanking region of yeast alcohol dehydrogenase I as a promoter to transcribe surface antigen coding sequences.
Abstract: The surface antigen of hepatitis B virus (HBsAg) has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5′-flanking region of yeast alcohol dehydrogenase I as a promoter to transcribe surface antigen coding sequences. The protein synthesized in yeast is assembled into particles having properties similar to the 22-nm particles secreted by human cells.

843 citations

Patent•
Adenovirus promoter system

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Lawrence S. Cousens1, Graeme I. Bell1, Pablo Valenzuela1•
Chiron Corporation1
18 Nov 1982
TL;DR: In this article, the adenovirus major late late promoter was employed as a promoter for expression in a yeast host and a coding segment was provided for yeast expression in yeast.
Abstract: The adenovirus major late promoter is employed as a promoter for expression in a yeast host. Constructions are provided for expression in yeast with the adenovirus major late promoter and a coding segment.

296 citations