Journal ArticleDOI
Synthesis and assembly of hepatitis B virus surface antigen particles in yeast
Pablo Valenzuela,Pablo Valenzuela,Angelica Medina,William J. Rutter,Gustav Ammerer,Benjamin D. Hall +5 more
TLDR
The surface antigen of hepatitis B virus has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5′-flanking region of yeast alcohol dehydrogenase I as a promoter to transcribe surface antigen coding sequences.Abstract:
The surface antigen of hepatitis B virus (HBsAg) has been synthesized in the yeast Saccharomyces cerevisiae by using an expression vector that employs the 5′-flanking region of yeast alcohol dehydrogenase I as a promoter to transcribe surface antigen coding sequences. The protein synthesized in yeast is assembled into particles having properties similar to the 22-nm particles secreted by human cells.read more
Citations
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Journal ArticleDOI
Foreign gene expression in yeast: a review.
TL;DR: In this article, the authors proposed a method to solve the problem of the "missing link" problem, i.e., "missing links" and "missing connections" problem.
Journal ArticleDOI
Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.
TL;DR: The Shine-Dalgarno sequence, appropriately positioned upstream from an exposed initiator codon, is sufficient to define a bacterial ribosome bnding site?
Journal ArticleDOI
The hepatitis B virus
TL;DR: In this paper, the hepatitis B virus (HBV) virology has been analyzed and recombinant DNA recombinant technology has been used for the development of new vaccines for hepatocellular carcinoma.
Journal ArticleDOI
Papillomavirus L1 major capsid protein self-assembles into virus-like particles that are highly immunogenic.
TL;DR: Results indicate that L1 protein has the intrinsic capacity to assemble into empty capsid-like structures whose immunogenicity is similar to infectious virions.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
H C Birnboim,J Doly +1 more
TL;DR: In this paper, a procedure for extracting plasmid DNA from bacterial cells is described, which is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day, yet yields DNA which is pure enough to be digestible by restriction enzymes.
Arapid alkaline extraction procedure forscreening recombinant plasmid DNA
TL;DR: The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction enzymes, and achievesequate pH control without using a pH meter.
Journal ArticleDOI
A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels
TL;DR: A simple method for detecting 3H in polyacrylamide gels by scintillation autography (fluorography) using X-ray film, which is ten times more sensitive than conventional autoradiography of isotopes with higher emission energies.