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Showing papers by "Davangere University published in 2014"


Journal ArticleDOI
TL;DR: Trichoderma harzianum may act as solution for the problem caused by polyethylene in nature as this organism was isolated from local dumpsites of Shivamogga District for use in the biodegradation ofpolyethylene.
Abstract: Trichoderma harzianum was isolated from local dumpsites of Shivamogga District for use in the biodegradation of polyethylene. Soil sample of that dumpsite was used for isolation of T. harzianum. Degradation was carried out using autoclaved, UV-treated, and surface-sterilized polyethylene. Degradation was monitored by observing weight loss and changes in physical structure by scanning electron microscopy, Fourier transform infrared spectroscopy, and nuclear magnetic resonance spectroscopy. T. harzianum was able to degrade treated polyethylene (40 %) more efficiently than autoclaved (23 %) and surface-sterilized polyethylene (13 %). Enzymes responsible for polyethylene degradation were screened from T. harzianum and were identified as laccase and manganese peroxidase. These enzymes were produced in large amount, and their activity was calculated using spectrophotometric method and crude extraction of enzymes was carried out. Molecular weight of laccase was determined as 88 kDa and that of manganese peroxidase was 55 kDa. The capacity of crude enzymes to degrade polyethylene was also determined. By observing these results, we can conclude that this organism may act as solution for the problem caused by polyethylene in nature.

57 citations


Journal ArticleDOI
TL;DR: A rich source of camptothecine and its natural derivatives, Pyrenacantha volubilis (Icacinaceae) from the eastern coast of peninsular India is reported and Seed extracts were found to be effective against breast cancer, ovarian, colon and carcinoma cell lines.

18 citations


Journal ArticleDOI
TL;DR: In this paper, the variation of optical band gap of PVA polymer with the addition of Fe3+ transition metal ion has been determined by the Tauc plot method, which is supported by conductivity obtained for these polymers and FT-IR spectra.

17 citations


20 Aug 2014
TL;DR: Under these optimised conditions the consortiumGSa and GSb had the potential to degrade 2,4 CB, a PCB congener, perhaps a candidate for in-situbioremediation.
Abstract: Pseudomonas spp consortium(GSa and GSb) was optimisedto degrade 2,4 dichlorbiphenyl (2,4 CB) in shakeflask culture The optimisation parameters such as concentrationof the substrates, rotations per minute, effectof substrate, concentration of sodium glutamate, pH,temperature, for degradation of 2,4 dichlorobiphenylwere evaluated The maximum degradation of 2, 4 CBwas observed under optimised conditions such as 35oC,pH 7, rpm- 130, and the concentration of 2,4 CB was90 ppm Supplimentation of 02% sodium glutamateand Surfactant Tween 80 resulted in highest amount of2,4 CB degradation which was measured spectrophotometricallyThe amount of chloride release with increasein biomass was estimated byMohr’s argentometricmethod Under these optimised conditions the consortiumGSa and GSb had the potential to degrade 2,4 CB, a PCB congener, perhaps a candidate for in-situbioremediation

3 citations