Showing papers by "Kagawa University published in 1972"
TL;DR: Using the procedure employed for the separation of fusarenon and nivalenol, toxic substances were separated from cereal grains infected with Fusarium in the crop field of Kagawa-ken in 1970 and fusarium roseum or related species were found to be a majority of casual fungi.
Abstract: Using the procedure employed for the separation of fusarenon and nivalenol, toxic substances were separated from cereal grains infected with Fusarium in the crop field of Kagawa-ken in 1970. Production of the toxic substances in culture filtrates of the isolated strain was also determined. The results are summarized as follows:(1) Fusarium spp., Alternaria spp., Aspergillus spp. and Penicillium spp. were isolated from moldy grains of barley and wheat. Fusarium roseum or related species were found to be a majority of casual fungi.(2) Moldy grains mainly contained “Rd-toxin”, mp. 149. 5-151°C, and nivalenol, mp. 224-226°C, both toxic to mice and protozoa and the former was considered as a newly found mycotoxin having a scirpene nucleus.(3) Phytotoxic butenolide, mp. 115-117°C, hardly detectable in the grains, and Rd-toxin were separated from culture filtrates of the isolated Fusarium roseum strain, whereas nivalenol was not detected.
86 citations
TL;DR: Besides p-NPP and phenylphosphate, various nucleotides, especially 2′ (or 3′) isomers, served as excellent phosphoryl donors, and various kinds of nucleosides and nucleotide were phosphorylated to form nucleoside monophosphates and nucleosid diphosphates.
Abstract: Distribution of NAD phosphorylating reactions, phosphorylation through NAD kinase and phosphotransferase, was investigated. NAD kinase activity was distributed rather widely in bacteria, whereas phosphotransferase activity with p-NPP and NAD was limited to a few genera. Proteus mirabilis showed strong activity of phosphotransferase besides NAD kinase activity.Partial purification of the phosphotransferase was attempted. The enzyme preparation possessed phosphatase activity as well as phosphotransferase activity. Phosphorylation of NAD proceeded maximally under the conditions below pH 4.0. Cu2+ showed stimulating effect on the activity. Besides p-NPP and phenylphosphate, various nucleotides, especially 2′ (or 3′) isomers, served as excellent phosphoryl donors, and various kinds of nucleosides and nucleotides were phosphorylated to form nucleoside monophosphates and nucleoside diphosphates.
10 citations
TL;DR: Contrary to high activity on the synthetic galactoside, reaction velocity was small when the enzyme acted on lactose, and the enzymic activity was clearly inhibited by Hg2+.
Abstract: An acid β-d-galactosidase was purified from the culture filtrate of Corticium rolfsii IFO 6146 by a combination of QAE-Sephadex A-50 and SP-Sephadex C-50 chromatography. The maximum activity of the enzyme towards p-nitrophenyl β-D-galactopyranoside was found to be at pH 2.0 to 2.5 and the enzyme was fairly active at pH 1.5 to l.8. The enzyme was quite stable over a pH range 2.0 to 8.0 at 2°C for 72 hr. The enzymic activity was clearly inhibited by Hg2+. Km value was determined to be 3.84 × 10−4 m, and Vmax was calculated to be 6.9 μ moles per min per mg for p-nitrophenyl β-d-galactopyranoside. Contrary to high activity on the synthetic galactoside, reaction velocity was small when the enzyme acted on lactose.
8 citations
TL;DR: Temperature treatments were conducted from early August to mid-September with 5-year-old bearing Kaki fruit trees (Cultivar. Fuyu) grown in clay pots and the weight of a fruit and its color at the end of treatments were best at 15°C, followed by 20°, 25°, and 30°C in the order.
Abstract: Temperature treatments were conducted from early August to mid-September with 5-year-old bearing Kaki fruit trees (Cultivar. Fuyu) grown in clay pots.1. When the trees were treated at night with temperature of 15°, 20°, 25° and 30°C and left in the field in the daytime from August 3, their fruits on September 21 showed no appreciable difference in the weight of a fruit among different temperatures except 30°C at which the fruit was lightest. Fruit color was already light yellowish green at 25° and 30°C. The chlorophyll content of the peel was lowest and the soluble solid content of the juice was highest at 25°C, followed by 30°, 20°, and 15°C in the order. However, the fruit quality on November 10 was most superior at 25°C and most inferior at 30°C.2. When the trees were placed under constant temperature of 15°, 20°, 25°, and 30°C throughout day and night of August 6 to September 18, the weight of a fruit and its color at the end of treatments were best at 15°C, followed by 20°, 25°, and 30°C in the order. The fruit at 30°C was still green. However, the fruit quality on October 25 was most superior at 20°C and most inferior at 30°C. Brown specks resulted from the coagulation of soluble tannin substance developed more in flesh at 25° and 30°C than at 15° and 20°C.
7 citations
TL;DR: Comparison of the effects of a high fat and high protein diet on the capacity for glucose formation from pyruvate and glycerol was investigated in vivo and in vitro, with results indicating that the response of the activity of hepatic glucose-6-phosphatase with high dietary fat was more rapid than that of theActivity of renal glucose- 6-ph phosphataseWith high dietary protein.
Abstract: Comparison of the effects of a high fat and high protein diet on the capacity for glucose formation from pyruvate and glycerol was investigated in vivo and in vitro. Ratios of radioactivity incorporated from either pyruvate-3-14C or glycerol-l-14C into blood glucose to those into expired CO2 were higher in both groups fed the high fat and the high protein diet than those in a group fed a high carbohydrate diet. Gluconeogenesis from pyruvate and glycerol by liver slices were both increased significantly in rats fed the high fat diet, while feeding the high protein diet caused increase of renal gluconeogenesis from pyruvate and glycerol. The activities of hepatic and renal glucose-6-phosphatase(s) were changed in a similar fashion to changes in hepatic and renal gluconeogenesis, respectively.In addition, the response of the activity of hepatic glucose-6-phosphatase with high dietary fat was more rapid than that of the activity of renal glucose-6-phosphatase with high dietary protein. Furthermore, the intrap...
3 citations
TL;DR: In this article, the eating qualities of cooked rice stored in an electric jar were investigated and it was found that the texture of the sample stored for 14 hours was significantly different from that of control.
Abstract: Eating qualities of cooked rice stored in an electric jar were investigated.Hardness with a texturometer increased during storage, and adhesiveness did not change so remarkably. The difference between the texture of control and that of the sample stored for 20.5 hours was not significant. Change in color was observed, but very little after storage about a day. Flavor of the sample stored 14 hours was significantly distinguished from that of control. This concepted odor was not so bad as unacceptable.From these results, physical qualities of cooked rice stored in an electric jar a day is considered to be acceptable.
3 citations
TL;DR: In this paper, the authors studied infinite renormalization of the neutral scalar field interacting with a fixed source in four-dimensional space-time and showed its features distinct from both physical and mathematical points of view, according to the condition that the source function satisfies.
Abstract: 8l It will be worthwhile to study in a similar way another example with infinite renormalization, always bearing in mind the extension of the method of asymptotic fields applicable to more complex and more realistic cases. The simplest model towards this direction is the neutral scalar field in interaction with a fixed source. As is well known, the theory of neutral scalar field interacting with a fixed source in four-dimensional space-time shows its features distinct from both physical and mathematical points of view, according to the condition that the source func tion satisfies. Let w (k) = (k2 + m2Y/2 be the energy of a single boson with a posi tive mass m, and let (J) 112v (k) be proportional to the Fourier transform of the source function. Define the space Gn of the functions v (k) equipped with the norm
2 citations
TL;DR: The enzyme was stable over a pH range 4.0 to 7.0 at 5°C for 72 hr and relatively unstable at pH 1.1 to 2.0 as compared with endo-polygalacturonase, α-l-arabinofuranosidase and β-d-galactosidase produced by C. rolfsii.
Abstract: An α-d-galactosidase was purified from the culture filtrate of Corticium rolfsii IFO 6146 by a combination of QAE-Sephadex A-50 and SE-Sephadex C-50 chromatography. The purified enzyme was demonstrated to be free of other possibly interfering glycosidases and glycanases. The maximum activity of the enzyme towards p-nitrophenyl α-d-galactopyrano-side was found to be at pH 2.5 to 4.5, and the enzyme was fairly active at pH 1.1 to 2.0. The enzyme was stable over a pH range 4.0 to 7.0 at 5°C for 72 hr and relatively unstable at pH 1.1 to 2.0 as compared with endo-polygalacturonase, α-l-arabinofuranosidase and β-d-galactosidase produced by C. rolfsii. The enzymic activity was completely inhibited by Hg2+ and Ag+ ions, respectively. Km values were determined to be 0.16 × 10−3 m for p-nitrophenyl α-d-galactopyranoside and 0.26 × 10−3m for o-nitrophenyl α-d-galactopyranoside. The values of Vmax were also determined to be 26.6 μmoles and 28.6 μmoles per min per mg for p- and o-nitrophenyl α-d-galactopyranoside, re...
1 citations