Showing papers by "Universidade Estadual de Londrina published in 1992"
TL;DR: Cytogenetic analysis was performed on cultures of primary ependymal tumors with different degrees of malignancy obtained from four patients, none of whom had received therapy before karyotypic evaluation, finding the most common abnormalities were monosomy 17 and 22 in four cases and losses of sex chromosomes in three cases.
51 citations
TL;DR: This study carried out in the region of Londrina, Parana, Brazil in three different areas: a primary forest, a disturbed primary forest and an open area, finding A. lituratus was found in greater number in all the areas, showing significant variation in relation to the size of the forests.
Abstract: This study was carried out in the region of Londrina, Parana, Brazil in three different areas: a primary forest, a disturbed primary forest and an open area. Within 207 hours of capture, 990 individuals were collected, 939 of them being fruit-eating bats. Carollia perspicillata (Linnaeus, 1758), Sturnira lilium (Geoffroy, 1810), Platyrrhinus lineatus (Geoffroy, 1810) and Artibeus lituratus (Olfers, 1818) were the four most collected species in a total of 899 individuals. A. lituratus was found in greater number in all the areas, showing significant variation in relation to the size of the forests. The partition of food resources among the four most collected species was known through the analysis of their feces. The coexixtence of these species can be explained by their investing in different foods or their diverging time activity.
47 citations
36 citations
TL;DR: The cytogenetic findings based on G-banding in a pineocytoma detected in a 29-year-old woman and chromosomal study showed numerical alterations involving chromosomes X, 5, 8, 11, 14, and 22, structural alterations of chromosomes 1, 3, 12, and22, as well as various markers.
25 citations
TL;DR: The Physiology of chioride ion and its relationship to clinical disorders in small animall practice is reviewed and a new clinical approach is used to assess chloride ion changes after accounting for changes in free water.
Abstract: Summary
The Physiology of chioride ion and its relationship to clinical disorders in small animall practice is reviewed. Chioride is the major anion in the extracellular fluid and is important in the metabolic regulation of acid-base balance. A new clinical approach is used to assess chloride ion changes after accounting for changes in free water. Using this approach chloride disorders can be divided into corrected and artifactual. Changes in free water are solely responsible for the chioride ion changes in artifactual disorders, whereas in corrected chloride disorders, chloride ion itself changes. Corrected hypochioremia is associated with increases in the strong ion differece (SID) and metabolic alkalosis and is caused by administration of solution containing a high concentration of sodium relative to chioride (e.g., Sodium bicarbonate) or the excessive loss chioride relative to sodium (e.g., vomiting of stomach contents). Administration of chioride is correction of hypochioremic metabolic alkalosis. Corrected hyperchioremia is associated with a decreased SID and metabolic acidosis and is usually the result of excessive loss of sodium relative to chloride (e.g., diarrhea), chioride retention (e.g., renal tubular acidosis), or therapy with solutions containing a high concentration of chioride relative to sodium (e.g.,0.9% sodium chloride;3–24% hypertonic saline). Treatment with sodium bicarbonate should be attempted in patients with corrected hyperchioremia and a plasma pH beiow 7.2.
24 citations
22 citations
TL;DR: In this paper, a new method of growing electrochromic nickel oxide films by thermal decomposition of nickel nitrate was presented, which was very adherent and homogeneous, and had electro-chromic efficiencies comparable with films produced by other methods.
Abstract: Presentation of a new method of growing electrochromic nickel oxide films by thermal decomposition of nickel nitrate. The films were very adherent and homogeneous, and had electrochromic efficiencies comparable with films produced by other methods
17 citations
TL;DR: The high positivity ELISA rates for cysticercosis in the CSF and in the patients serum with epilepsy indicate that neurocysticercotic cause is an important seisure cause in Londrina, PR.
Abstract: Fifty patients with epilepsy seen in three Londrina Neurological Services, in Parana, were studied. The positivity prevalence of the enzyme-linked immunosorbent assay (ELISA) for cysticercosis in the cerebrospinal fluid (CSF) and in the serum of these patients was 34.0% and 20.0%, respectively. There was statisticaly significant difference when these two rates were compared with the reaction positivity in the CSF and the serum in the control group, formed by individuals without neurological diseases. There was no association between the type of seisure (generalized or partial) and the positivity index of ELISA for cysticercosis in the CSF. A greater number of patients with positive ELISA for cysticercosis in the rural area dwellers was found, in relation with the urban area dwellers. From the obtained results in our study we came to the following conclusions: 1. The high positivity ELISA rates for cysticercosis in the CSF and in the patients serum with epilepsy indicate that neurocysticercosis is an important seisure cause in Londrina, PR. 2. The positivity prevalence of ELISA for cysticercosis in CSF was greater in epileptic patients from the rural area than the ones from the urban area. 3. There was no association between the type seisure (generalized or partial) and the ELISA cysticercosis positivity rate in the CSF. 4. The high positivity prevalence of ELISA in the CSF and in the epileptic patients serum in Londrina indicates the priority of performing epidemiologic inquiry to establish the real cysticercosis prevalence in the city. ELISA may be used with this finality due to its high sensibility, its low cost and its simple performance.
14 citations
14 citations
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TL;DR: Results indicate that cell-bound hemolysin is not a main factor of virulence for mice in Serratia marcescens.
Abstract: 1. A total of 60 nosocomial isolates of Serratia marcescens were screened for the presence of markers related to virulence, i.e., cell-bound hemolysin and production of siderophore aerobactin. 2. No aerobactin-producing strains were found, and the incidence of cell-bound hemolysin was 97%. 3. Hemolysin-positive (58 strains) and hemolysin-negative (2 strains) Serratia marcescens showed the same LD50 (3 x 10(7) bacteria) in a test of virulence for mice. 4. These results indicate that cell-bound hemolysin is not a main factor of virulence for mice in Serratia marcescens.
14 citations
Journal Article•
TL;DR: The results indicate that mannose receptors on the surface of phagocytic cells mediate E. coli or Candida albicans uptake and that the binding of bacteria to N-acetylgalactosamine residues from the membrane ofphagocytes is also involved in thephagocytosis of E. Escherichia coli.
Abstract: 1. Ingestion of enteropathogenic Escherichia coli or Candida albicans by thioglycollate-elicited macrophages and polymorphonuclear leukocytes was investigated in vitro, 2. Goat antiserum against mannose receptors caused about 50% inhibition of E. coli phagocytosis and about 90% inhibition of C. albicans phagocytosis. 3. E. coli and C. albicans uptake was inhibited by about 60% and 98%, respectively, by plating the macrophages onto substrates coated with poly-L-lysine-mannan. Further addition of 50 mM mannose to the medium significantly increased the inhibition of phagocytosis of E. coli by macrophages from 60.7 +/- 1.5 to 79.8 +/- 13.1 and by polymorphonuclear cells from 58.9 +/- 3.7 to 88.7 +/- 4.9. 4. Preincubation of phagocytic cells with antiserum against substance A of human erythrocytes reduced E. coli ingestion by 95%, but this inhibition was not observed when the antiserum was incubated with N-acetylgalactosamine (50 mM) before being added to the phagocytes. The phagocytosis of C. albicans was not inhibited by anti-substance A antiserum. 5. The phagocytosis of E. coli was inhibited by about 25% by the addition of 7.8 micrograms/ml soluble mannan to the medium, and by about 50% by the addition of 50 mMN-acetylgalactosamine; when both substances were added to the medium, an additive inhibition of about 75% was observed. 6. These results indicate that mannose receptors on the surface of phagocytic cells mediate E. coli or Candida albicans uptake and that the binding of bacteria to N-acetylgalactosamine residues from the membrane of phagocytes is also involved in the phagocytosis of E. coli.
01 Jan 1992
Journal Article•
TL;DR: The use of the reagent, p-benzoquinone (PBQ) for the spectrophotometric determination of total proteins in blood plasma is documented and an absorption band at 350 nm follows Beer's law, which can be used for analytical purposes.
Abstract: 1. In the present study we have documented the use of the reagent, p-benzoquinone (PBQ) for the spectrophotometric determination of total proteins in blood plasma. 2. Since the products of reaction are stable for several hours at room temperature after the 20-min boiling step, the time at which absorbance is measured is not a critical factor. 3. Common anticoagulants such as EDTA, citrate, or heparin do not interfere with the PBQ method at concentrations used in clinical laboratories. 4. The products of the reaction between PBQ and either plasma (specific absorbance 2.33 x 10(-3) +/- 0.20 x 10(-3) micrograms cm-2) or purified proteins (specific absorbance 2.61 x 10(-3) +/- 0.31 x 10(-3) micrograms cm-2) show an absorption band at 350 nm, which follows Beer's law, and therefore can be used for analytical purposes. 5. The PBQ method has a lower limit of detection (4 micrograms/ml) than that of the biuret method (45 micrograms/ml) for a final reaction mixture of 5.0 and 4.2 ml, respectively.
TL;DR: The authors of the search propose a training systematics to the recently graduate on his first employment that can be used for the employer institutions, with the intention of decreasing the nursing professionals worries on the start of their profession.
Abstract: Os autores da pesquisa baseados em informacoesde enfermeiros recemgraduados e dados da literatura, propoem uma sistematica de treinamento para o recem-formado em seu primeiro emprego, que pode ser utilizada pela instituicao empregadora, com a finalidade de diminuir as ansiedades dos profissionais de enfermagem no inicio de suas carreiras.
Journal Article•
TL;DR: The results suggest that proteinases released from C. albicans are involved in the early killing of macrophages.
Abstract: 1. We have investigated the possibility that proteinases released by Candida albicans participate in the early killing of three types of mice peritoneal macrophage (resident, thioglycollate-elicited, or Con A-activated) in vitro. 2. Phagocytic assays were performed by incubation of macrophages and C. albicans together at a 1:10 ratio for 30 min at 37 degrees C in RPMI medium buffered to pH 7.0 with 12 mM Hepes without serum. With no albumin added to the medium, the macrophages were 85% to 100% damaged and unviable, to a greater extent than expected from the proportion of phagocytic cells containing germ tubes. When 10 mg/ml of albumin was added to the medium, however, 90% of the macrophages remained viable for the 2 hours of the phagocytic assay, suggesting that albumin may have acted as a substrate for or inhibitor of proteinases released by C. albicans, thereby protecting the macrophage from the proteolytic action of the proteinases. 3. The phagocytosis of IgG-coated erythrocytes was reduced to 43% when IgG was preincubated with the supernatant from C. albicans cultures, but the addition of 10 mg/ml albumin or of 5 micrograms/ml pepstatin (an inhibitor of C. albicans acid proteinases) to the same supernatants prevented the effect on phagocytosis of IgG-coated erythrocytes. 4. These results suggest that proteinases released from C. albicans are involved in the early killing of macrophages.
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01 Jan 1992TL;DR: O artigo do Prof. Dr. Jurandyr Luciano Sanches Ross que busca facilitar o conhecimento do Relevo Brasileiro atraves de uma nova proposta de classificacao as mentioned in this paper.
Abstract: Os estudos geograficos estao em plena expansao no Brasil, o que e revelado por um lado, pela quantidade de trabalhos de pesquisa sobre os mais variados temas, e por outro lado, o que e mais importante, pelo aumento progressivo das reflexoes sobre as implicacoes metodologicas e tecnicas da sua propria realizacao. E neste contexto que colocamos o artigo do Prof. Dr. Jurandyr Luciano Sanches Ross que busca facilitar o conhecimento do Relevo Brasileiro atraves de uma nova proposta de classificacao.
Journal Article•
TL;DR: In this paper, a set of invariant metafisicos that giram em tomo da questao central do Ser, explorando certos aspectos comuns na fronteira da Filosofia, da Psicologia e da Matematica, were investigated.
Abstract: Normal 0 21 MicrosoftInternetExplorer4 Neste estudo n6s desenvolvemos alguns invariantes metafisicos que giram em tomo da questao central do Ser, explorando certos aspectos comuns na fronteira da Filosofia, da Psicologia e da Matematica.