Showing papers in "Analytical Biochemistry in 1968"

TL;DR: A simple spectrophotometric method for the routine concomitant determination of sulfhydryl groups in PB- SH, NP-SH, and T-SH fractions in various tissues is reported.

TL;DR: A modified method for enzymic synthesis of UDP-14C-glucose in high yield is described, and it was found that stopping the transferase-catalyzed reaction by precipitation of trichloroacetic acid insoluble protein resulted in a loss of radioactive glucose.

TL;DR: A modified and updated description of the author's method for the assay of intestinal disaccharidase activity is presented, and the glucose liberated is measured with a glucose oxidase reagent.

TL;DR: A fluorometric hydroxyindole assay method for a wide range of catecholamines and related compounds has been developed based on iodine oxidation, alkaline rearrangement, and subsequent measurement of the fluorescence of the final solution at an acid pH.

TL;DR: In this article, a method for direct determination of sucrose is described, which depends on the destruction of reducing sugars with hot alkali, followed by determination of the fructose moiety of the sucrose, using cold anthrone.

TL;DR: A spectrophotometric method which eliminates interference due to nucleic acid absorbance has been developed for determining protein concentration over the range of 5 to 180 μg/ml and the results obtained have been compared to those obtained with the Folin-Lowry and microbiuret methods.

TL;DR: The carbazole analysis when run at 55° in the presence of borate gives increased sensitivity for d -galacturonic, d -mannuronic and l -guluronic acids, while greatly decreasing that of d -glueuronic acid.

TL;DR: A method for the preparation and use of continuous linear concentration gradients of polyacrylamide gel is described in detail and electrophoresis to an “end point” of complete arrest of protein molecules by gel pores is possible and should yield direct information about dimensions and the shape ofprotein molecules.

TL;DR: An apparatus is described which allows acrylamide gel electrophoresis to be carried out on a thin flat gel sheet, instead of a thick gel slab, or a cylindrical gel core.

TL;DR: Very small amounts of sulfo- and galacto-lipids can be reproducibly estimated by this method and the sugar component is estimated by the reaction of the lipid on the thin-layer adsorbent with phenol and sulfuric acid.

TL;DR: Chlorpromazine and some of its metabolites have been identified in human blood with the combination of gas chromatography and mass spectrometry and a new method, mass fragmentography, based upon a continuous recording of up to three mass numbers characteristic of a single substance or a group of compounds is elaborated.

TL;DR: A convenient method is described for the preparation for liquid scintillation spectrometry of slices of polyacrylamide gel containing isotopically labeled protein, with high efficiency and recoveries of radioisotope usually exceed 90%.

TL;DR: The chromatographic properties on molecular sieve columns of polypeptides from collagen were investigated under conditions in which the polypePTides are random coils and an acrylamide gel was found suitable for the separation and molecular weight determination.

TL;DR: The major nucleosides obtained by hydrolysis of RNA are quantitatively assayed in less than 1 hr at the level of 1 or more nmoles by cation-exchange chromatography and a sensitive, continuous ultraviolet-absorbance monitoring device, and analysis sensitivity is enhanced by improved flow cell and electronic circuit designs.

TL;DR: The electrophoretic mobility in barium acetate thus depends primarily on the structure of the polysaccharide backbone, whereas the sulfate content is of less importance.

TL;DR: The procedure is rapid and more sensitive than established procedures; an accurate assay can be performed on a single tissue sample containing 20 ng of noradrenaline, 30 ng of serotonin, and 100 ng of dopamine.

TL;DR: Oxidized glutathione content (average ± standard deviation) of human, rabbit, and rat erythrocytes has been shown to be 3.6 ± 1.6 mμmoles/ml RBC, and the GSSG content of rat liver, kidney, heart, lens, testes, and skeletal muscles is below the level of detection.

TL;DR: Ultrafiltration membranes capable of retaining macromolecules, but permeable to micro solutes, have been used in a fixed volume diafiltration assembly and the system permits extremely rapid micro solute exchange in protein solutions and is also applicable to the study of binding behavior.

TL;DR: The general analytical and kinetic characteristics of the hexokinase/G6PD method showed that the method may be used accurately at ambient temperatures, with a straight line standard curve, at an incubation time of 10 min, for sample concentrations up to 100 mg 100 ml.

TL;DR: A technique for the preparation, analysis, and fractionation of macromolecules using a computer-calculated isokinetic sucrose gradient is described and the results using rat liver ribosomes, polysomes, and ribosomal RNA are presented.

TL;DR: The method is suitable for the assay of d -amino acids in the presence of the l isomers, and is also applicable for the determination of d-amino acid oxidase activity.

TL;DR: The radioactive dye technique allows much more accurate determinations of degree of labeling, as well as of the extinction coefficient, which may be of interest in itself.

TL;DR: The colorimetric method gives results proportional up to 20 mg % of uric acid, and the amount of brown color formed is measured at 436 nm.