Journal ArticleDOI
An improved method for counting tritium and carbon-14 in acrylamide gels
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This article is published in Analytical Biochemistry.The article was published on 1968-10-10. It has received 105 citations till now. The article focuses on the topics: Acrylamide.read more
Citations
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Myosin synthesis in cultures of differentiating chicken embryo skeletal muscle.
TL;DR: It is tentatively concluded that, in the regulation of chicken skeletal muscle myogenesis, cell fusion potential and the potential for myosin synthesis are developed not simultaneously, but sequentially.
Journal ArticleDOI
A Myogenic Cell Line with Altered Serum Requirements for Differentiation
David Yaffe,O. Saxel +1 more
TL;DR: Dfferentiation properties of a cell line, L84, which originated from a non-fusing clone isolated from the myogenic line L8, are described and show that L84 cells retain the programme for their differentiation into muscle fibres.
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Amidination of the outer and inner surfaces of the human erythrocyte membrane.
TL;DR: There are more than ten times as many reactive amino groups in protein on the inner surface than on the outer surface of the membrane, which implies that the isolation of the human erythrocyte does not load to major structural rearrangements.
Journal ArticleDOI
Examination of the Protein Composition of the Cell Envelope of Escherichia coli by Polyacrylamide Gel Electrophoresis
TL;DR: An envelope preparation containing the cell wall and cytoplasmic membrane of Escherichia coli was obtained by breaking the cells with a French pressure cell and sedimentating the envelope fraction by ultracentrifugation, suggesting that this organism is able to adapt to changes in growth environment with only minor modifications of the major proteins of the cell envelope.
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Replication of tobacco mosaic virus: III. Viral RNA metabolism in separated leaf cells☆☆☆
TL;DR: Results of these studies suggest that RI and RF may be progenitors of TMV RNA; however, the inability to completely chase [ 3 H]uridine from RF raises additional possibilities, which are discussed.
References
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Journal ArticleDOI
Resolution of Multiple Ribonucleic Acid Species by Polyacrylamide Gel Electrophoresis
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Acrylamide-gel electrophorograms by mechanical fractionation: radioactive adenovirus proteins.
TL;DR: An electrophorogram of radioactive type-2 adenovirus proteins so fractionated gave a pattern in excellent agreement with the pattern obtained by laborious manual sectioning and in agreement withThe pattern obtained on a replicate gel stained with Coomassie brilliant blue R250.
Journal ArticleDOI
A convenient method of preparing polyacrylamide gels for liquid scintillation spectrometry
TL;DR: A convenient method is described for the preparation for liquid scintillation spectrometry of slices of polyacrylamide gel containing isotopically labeled protein, with high efficiency and recoveries of radioisotope usually exceed 90%.
Journal ArticleDOI
Improved separation of serum proteins in paper electrophoresis: a new electrophoresis buffer.
T. Aronsson,A. Grönwall +1 more
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An acrylamide gel soluble in scintillation fluid: Its application to electrophoresis at neutral and low pH
Choules Gl,Zimm Bh +1 more
TL;DR: Two additions to diacrylamide gel techniques are presented, one of which is the development of a catalyst system suitable for making pH 2.5 gels containing 1 M propionic acid as the electrolyte.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more