Showing papers in "Biology of the Cell in 1986"

TL;DR: The transformation of dense fibrillar primary nucleoli into functional reticulated nucleoli appeared sooner in the development of cow embryos than in other mammalian species hitherto studied and took place generally during the 8‐cell stage.

TL;DR: A critical survey of the routine use of rhodamine 123 together with flow cytometry in mitochondrial research is presented and reveals a useful probe for monitoring the abundance and activity of mitochondria.

TL;DR: Xenopus oocytes were stained by anti‐tubulin and anti‐MAP1 antibodies during the first meiotic cell division and a microtubular network appears at the basal part of the disrupting nucleus, which is cold, calcium‐ and antimitotic drug sensitive.

TL;DR: Localisation infracellulaire du cadmium, discussion sur sa mise en reserve and sur les mecanismes possibles de detoxication.

TL;DR: It is shown that fusion of human myoblasts and differentiation of myotubes in tissue culture can be accelerated by removal of serum macromolecules.

TL;DR: Ultrastructurally, the intracellular lumen appears as a more or less spherical cavity with osmiophilic substance, bordered with microvilli and surrounded by a filamentous network.

TL;DR: This model proposed that steroid hormone receptors existed under two different forms: the unliganded receptor in the cytoplasm and the hormone-bound receptor complex in the nucleus, and a nuclear translocation mechanism was hypothesized as a necessary link between the two forms.

TL;DR: Fibronectin and their cell surface receptor constitute the major cell‐substratum adhesion that plays a key role in cell adhesion, cell spreading and cell motility, particularly during embryonic development.

TL;DR: Following PEG (polyethylene glycol) treatment of ovulated metaphase II mouse oocytes aggregated with thymocytes, fusion of cell membranes occurs and an extensive polymerization of microtubules starts in the center of the chromatin mass which leads to the formation of an apparently non‐functional spindle‐like structure.

TL;DR: Isolated cortices from sea urchin eggs accumulate calcium in a non‐mitochondrial store at a rate dependent on ATP and enhanced by oxalate, and calcium efflux is increased by ionomycin and by A23187.

TL;DR: It is suggested that a single NOR of pig embryo kidney cells corresponds to one “fibrillar complex”, i.e., to an association of a different number of FCs with the dense fibrillAR component.

TL;DR: Ten distinct DNA sequences that hybridized strongly to the heterologous collagen probe are characterized and it is shown that these sequences are dispersed throughout the Drosophila genome.

TL;DR: It is suggested that during SV40 lytic infection, HMG1 accumulates at sites of active viral DNA replication, and its presence in the nucleus is related to its requirement in the DNA replication process.

TL;DR: Cilia from quail oviduct were isolated with their membrane and the ultrastructural study revealed a good preservation of cilia in the purified fraction, suggesting an heterogeneous distribution of actin within the axonemal length.

TL;DR: The fluorescence properties of fluorescein bound to protein are used to quantitate by flow cytofluorometry the degradation of fluocytosed neoglycoprotein after endocytosis by the membrane lectin of Lewis lung carcinoma cells (3LL cells).

TL;DR: The evolution of SCO ependymocytes coincided with the progressive onset and maturation of axo‐glandular innervation taking place after birth, and it was found that the increase in the serotonin‐immunoreactive fiber density in the whole organ was time‐dependent.

TL;DR: It was demonstrated that the resistance of these yeasts to a large spectrum of antibiotics was due to several features: a resistance to oligomycin was found at the permeation level; the resistance to the other drugs was correlated to the relative insensitivity of cytochrome biosynthesis to the drugs.

TL;DR: The functional role of decidual and metrial gland cells is discussed in relation to their structural complexity and recent observations on the haemopoietic origin of these cells.

TL;DR: It is confirmed that azide inhibits the recycling of the receptors and evidence for the involvement of thiol groups in the internalization step is provided and pretreatment of hepatocytes with azide allows us to demonstrate that receptor endocytosis proceeds independently of the presence of any specific ligand.

TL;DR: The results suggest the presence of at least one type of lectin‐like protein with an extended binding site accomodating several sugar units, and define its localization on the parasite surface.

TL;DR: The thermostability of the two enzymes was different: arylulfatase B was found to be more stable than arylsulfat enzyme A, which was inhibited by sulfate, sulfite, silver, magnesium, manganese and calcium ions.

TL;DR: The results of the present study strongly suggest that the DNA molecules of the chromosomes bearing ribosomal genes have many sites of attachment to a non‐chromatin nucleolar network which can be referred to as a nucleolar skeletal complex.

TL;DR: Four monoclonal antibodies have been produced that specifically react with a rabbit intestinal brush‐border glycoprotein which migrates in SDS‐polyacrylamide gel electrophoresis as a protein of molecular weight 140,000.

TL;DR: The wound healing process of frog skin fragments in epibolic cultures has provided information on FN localizations during the migration of keratinocytes, and it was worth noting that fibronectin was permanently detected in the subcutaneous tissue of non‐cultured or cultured skin fragments with or without CHX.

TL;DR: The distribution of the herpes simplex virus alkaline nuclease in rabbit fibroblast cells infected with HSV type 1 or 2 (HSV‐1 or HSV‐2) has been examined with the aid of immunocytochemical techniques using gold particles as markers and nucleolar 100 kDa protein was found to be simultaneously present in the dense bodies.

TL;DR: Electron Probe Microanalysis (EPMA) has shown that aluminum concentration occurred in cells of the reticulo‐endothelial system, principally in the reticular cells of erythroblastic islets of rats intoxicated by aluminum gluconate, and the consequences of the selective accumulation of aluminum in the cytoplasm of reticularocytes for the maturation of ERYthrocytes are discussed.