P
Patrick Midoux
Researcher at Centre national de la recherche scientifique
Publications - 81
Citations - 2916
Patrick Midoux is an academic researcher from Centre national de la recherche scientifique. The author has contributed to research in topics: Gene delivery & Transfection. The author has an hindex of 31, co-authored 81 publications receiving 2675 citations. Previous affiliations of Patrick Midoux include University of Orléans.
Papers
More filters
Journal ArticleDOI
Uptake of neoglycoproteins via membrane lectin(s) of L1210 cells evidenced by quantitative flow cytofluorometry and drug targeting
TL;DR: Fucosylated serum albumin was shown to be the most efficient neoglycoprotein carrier, and to have a cytotoxicity close to that of anti L1210 cell IgM monoclonal antibody carrying methotrexate.
Journal ArticleDOI
Sonoporation: mechanistic insights and ongoing challenges for gene transfer.
TL;DR: Current state of the art concerning microbubble-cell interactions and cellular effects leading to sonoporation and its application for gene delivery are summarized.
Journal ArticleDOI
Macropinocytosis of polyplexes and recycling of plasmid via the clathrin-dependent pathway impair the transfection efficiency of human hepatocarcinoma cells.
Christine Gonçalves,Eric Mennesson,Renate Fuchs,Jean-Pierre Gorvel,Patrick Midoux,Chantal Pichon +5 more
TL;DR: It is demonstrated that macropinocytosis of polyplexes and plasmid recycling impair the transfection efficiency and clathrin-dependent endocytotic is the most productive route for transfections of HepG2 cells.
Journal ArticleDOI
Gene transfer by DNA/glycosylated polylysine complexes into human blood monocyte-derived macrophages.
Patrick Erbacher,Marie-Thérèse Bousser,Jacques Raimond,Michel Monsigny,Patrick Midoux,Annie Claude Roche +5 more
TL;DR: The possibility of transferring and expressing an exogenous gene into macrophage-like cells by using a nonimmunogenic synthetic vector as a DNA carrier opens new ways to develop nonviral gene therapy strategies.
Journal ArticleDOI
Glycosylated polylysine/DNA complexes: gene transfer efficiency in relation with the size and the sugar substitution level of glycosylated polylysines and with the plasmid size.
TL;DR: A DNA delivery system based on the use of polylysine substituted with small recognition signals, such as carbohydrate moieties specifically recognized by membrane lectins present in a given cell line, has been developed and the strength of the electrostatic interactions between the plasmid and the glycosylatedpolylysine plays an important role in the efficiency of the gene expression.