Showing papers in "China Journal of Modern Medicine in 2009"

Matrix metalloproteinase-9 polymorphism (C1562T) in patients with cerebral infarction

Abstract: 【Objective】To investigate the association between the susceptibility of cerebral infarction in Chinese population and matrix metalloproteinase-9 (MMP-9) polymorphism (C1562T). 【Methods】A case-control study of seventy patients with cerebral infarction (cerebral infarction group) and sixty control subjects (control group) was conducted. Plasma levels of MMP-9 were measured by enzyme-linked immunosorbent assay (ELISA). Promoter C1562T gene polymorphism of MMP-9 was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Genotype and allele frequency were compared between cerebral infarction group and control group. 【Results】Plasma MMP-9 level in cerebral infarction group was significantly higher than that of control group (P 0.01). The frequency of CT genotype and T allele were higher in cerebral infarction group compared with those in control group (P 0.05). Plasma MMP-9 level of CT/TT genetype was higher than that of CC genetype(P 0.01). 【Conclusion】The present findings suggest that the genetic polymorphism in MMP-9 promoter (C1562T) might be associated with the susceptibility of cerebral infarction in Chinese population.

Study on construction of nano tPA plasmid to prevent thrombosis after mechanical valve replacement in dogs

Abstract: 【Objective】To elucidate the transfection of nano tPA gene plasmid to prevent thrombosis after tricuspid mechanical valve replacement in dogs【Methods】A dog model of tricuspid mechanical valve replacement was constructedA constructed chitosan nano tPA gene plasmid was used to transfect the dog cardiocytes at the same time of tricuspid replacementThe effect of this gene on survival time of dogs , anticoagulation result(prothrombin time, INR and D-dimer contents, thrombosis in heart), the expressions of tPA were observed with the methods of clinical laboratory, routine pathology and immunohistochemical stain【Results】Nano tPA gene plasmid was successfully constructed and transfected into cadiocytesThe gene could significantly increased the survival time and serum level of D-dimer(P 001) and could prevent thrombosis of mechanical valve, but there was not significant difference of prothrombin time and INR between two groups(P 005)ConclusionThe constructed nano tPA gene plasmid could prevent thrombosis after mechanical valve replacement, which provide the bases for prevention of human disease using genetic drugs

Correlation between DNA repair gene XRCC1 Arg280His polymorphism and susceptibility to hepatocellular carcinoma in Fusui county of Guangxi

Abstract: 【Objective】To study the correlations of DNA repair gene XRCC1 Arg280His polymorphism and genetic susceptibility to hepatocellular carcinoma (HCC) between the population of Zhuang and Han Nationality in Fusui County of Guangxi.【Methods】Case-Control study were adopted for 50 patients with HCC and 30 health controls in local high-risk region, Arg280His genotypes frequencies and allelic frequencies were analyzed by PCR- RFLP and sequencing.【Results】Compared between the population of Zhuang and Han Nationality, the frequencies of mutant 280His alleles were 11% and 13% in the case group, respectively, and 10% and 11.7% in the controls group; there was no significant difference between the 2 groups (P 0.05).All the polymorphic genotypes distribution met the Hardy-Weinberg equilibrium. 【Conclusion】There is no obvious correlation for XRCC1 Arg280His polymorphism and HCC between Zhuang and Han Nationality in Fusui.

Effects of new sulfonylurea compound G004 on glycometabolism in insulin-resistance HepG2 cells

Abstract: 【Objective】To investigate the effect of new sulfonylurea compound G004 on glycometabolism in in-sulin-resistance HepG2 cells【Methods】HepG2 cells were incubated with 5 ×10-7 mol/L insulin for 16 h to estab-lish insulin-resistance(IR-HepG2)The effects of new sulfonylurea compound G004 on glucose consumption(GC),synergic action of insulin,glycogen synthesis,gluconeogenesis and activity of PEPCK were tested in this model re-spectively【Results】Compared with model group,G004 could noninsulin-dependently increase GC in insulin-re-sistance HepG2 cells at high,medium and low levels of glucose respectivelyIt enhanced IR-HepG2 cells glyco-gen synthesis significantlyG004 inhinited gluconeogenesis from lactic acid and activity of PEPCKHowever,this study showed glimepiride did not facilitate gluconeogenesis and PEPCK activity of HEPG2 cells obviously【Con-clusion】Compound G004 had remarkable effect on improving glycometabolism in IR-HepG2 cells

Inhibitory effects of salvianolic acid B on myocardial cellular nuclear transfer of NF-κB p65 and the expression of TNF-α during ischemia-reperfusion in rabbits hearts in vivo

Abstract: [Objective]To investigate the effects of salvianolic acid B (SA-B) on the activity of nuclear transfer of nuclear factor -κB p65 (NF-κB p65) in myocardial cells and the expression of tumor necrosis factor-alpha (TNF-α) during ischemia-reperfusion in rabbit models. [Methods]Thirty-two anesthetized New Zealand white rabbits were randomly divided into four groups: sham operation group, ischemia-reperfusion group, low dose SA-B treatment group and high dose SA-B treatment group. For sham operation group, the animals were not subjected to occlusion of LAD. Rabbits in ischemia-reperfusion group and SA-B treatment group underwent 0.5 hour of LAD occlusion followed by 4 hours of reperfusion. After LAD ligation rabbits in low and high dose SA-B treatment groups were im-mediately intravenously administered with low and high dose SA-B respectively, while rabbits of other two groups were given 0.9% sodium chloride in the same manner. Myocardial tissues from different groups were collected 4 hours after reperfusion. The amount of NF-κB p65 protein in nucleus of cardiomyocyte and the expression level of TNF-α in myocardial tissues were determined by western blot analysis and enzyme linked immunosorbent assay (ELISA), respectively. The activities of the myocardial myeloperoxidase (MPO) were detected by biochemical tech-niques. [Results]A higher amount of NF-κB p65 was observed translocated in cardiomyocyte nucleus in ischemia-reperfusion group than in sham operation group (P0.01). The expression levels of TNF-α and the activity of MPO were both significantly increased in ischemia-reperfusion group compared with sham operation group (P 0.01). Compared with ischemia-reperfusion group, the expressions of TNF-α were reduced in a dose-dependent manner in low and high dose SA-B treated groups (P0.05 or P0.01), as well as the amount of NF-κB p65 in nucleus and the MPO activity (P0.05 or P0.01). [Conclusion]The results indicated that salvianolic acid B has an effect of exten-uating myocardial cells injury during ischemia-reperfusion in some extent. This potential pharmacological action may be achieved through inhibiting the activation of NF-κB and decreasing the expression of TNF-α followed with re-ducing neutrophils infiltration in damaged myocardial tissues.

Effect of Warfarin thromboembolism prevention in patients with non-rheumatic atrial fibrillation

Abstract: 【Objective】To compare the thromboembolism effect of Warfarin with Aspirin in preventing non-valvular atrial fibrillation (AF) patients. 【Methods】The initial dose of Warfarin group is 2.0 mg/d, and adjusting the dose of warfarin and making the international normalized ratio (INR) stable in the 2.0 ~ 3.0. The dose of Aspirin group is 150 mg/d. AU patients are given conventional out-patient follow-ups. The primary endpoint events are ischemic stroke and death, and secondary endpoint events include peripheral arterial thrombosis, transient ischemic attack, asymptomatic cerebral infarction, acute myocardial infarction and severe bleeding. 【Results】Compared with Aspirin, Warfarin greatly decreased the rate of the primary endpoint events (3.3% vs 10.7%, P 0.05), and ischemic stroke relative risk dropped 69.5 %,and the relative risk of thromboembolism events dropped 71.3% (3.3% vs 11.5%, P 0.05). The total rate of bleeding in Warfarin group was significantly higher than in Aspirin group (9.8% vs 2.5%, P 0.05). 【Conclusions】 Warfarin can effectively reduces the rate of thromboembolism in non-valvular atrial fibrillation patients, although the rate of bleedingis higher than that of Aspirin group. Warfarin is still safe and effective if INR carefully monitored.

Clonorchis sinensis eggs involved in the formation of gallbladder stones

Abstract: Objective To investigate the relationship between clonorchiasis infection and cholecystolithiasis. Methods In ourpresent study, we chose 204 portions ofgallbladderstones by random from 204 patients who accepted gallbladder-retention cholelithotomy during 2006.12～2009.01 at general surgery department in our hospital, and then we grinded them to explored the internal structure and construction. Meanwhile, 108 portions of homochronous bile were chose randomly, and bile sediment was obtained through centrifugalization, and then slides were smeared and tested by microscopy. Results 163 of the 204 cases of gallbladder stones were detected C. sinensis eggs in the core (79.9%), while 46 of the 108 cases of bile could be detected C. sinensis eggs (42.6%), and they had obvious statistical difference (P 0.0001). The detection rate of C. sinensis eggs was 89.6%, 57.9% and 70.0% in the pigment calculus, cholesterol and mixed calculus respectively, which had obvious statistical difference (P 0.0001). 159 of the 204 cases were from clonorchiasis-high-incidence area, such as Guangdong and Guangxi (Pigment calculus, cholesterol and mixed calculus occupied 69.2%, 5.7% and 25.2% respectively), 45 of them were from clonorchiasis-low-incidence area, such as Gansu and Sichuan (Pigment calculus, cholesterol and mixed calculus occupied 11.1%, 22.2% and 66.7% respectively.), the constituent ratio of the two groups had obvious statistical difference (P 0.0001). ConclusionsOur data for the first time demonstrated that clonorchiasis infection had a close relationship with Cholecystolithiasis: we found that it was mainly pigment calculus in clonorchiasis-high-incidence area and cholesterol and mixed calculus in clonorchiasis-low-incidence area and the detection rate of C. sinensis eggs in pigment calculus was much higher than that in cholesterol and mixed calculus; microscopic examination of gallbladder stones maybe the economical, easy and effective method for the diagnosis of clonorchiasis infection.

Expression of decay receptor 3 and the effect on angiogenesis in hepatic cell carcinoma

Abstract: 【Objective】To study the expression of DcR3 and the effect on angiogenesis in HCC 【Methods】The expression of DcR3 was detected by RT-PCR in 40 patients with HCC,40 normal tissues adjacent to tumor and 20 normal tissues Flow cytometer was used to test cell apoptosis The immunohistochemistry (S-P) was used to test the expression of CD34 in HCC,and recorded as microvessel density (MVD) 【Results】DcR3 mRNA was detected in 27 (675%) of 40 HCC,while was not seen in tissues adjacent to tumor and normal control The mean apoptosis rate of HCC was higher than those of tissues adjacent to tumor and normal tissues There were no significant relationship between DcR3 and sex or age (P 005) But in situation of bigger tumor,TNM higher degree,lymphatic follicle metastasize,and portal vein encroached,the expression of DcR3 increased (P 005) The vascular endotheliocyte cell was stained yellow in HCC,and MVD was higher than those of tissues adjacent to tumorand normal tissues MVD in DcR3 positive group was higher than that of DcR3 negative group (P 005) 【Conclusion】DcR3 expression can be highly found in gastric carcinoma The abnormal expression of DcR3 may promote tumorigenesis and progression DcR3 may be important in evaluating the tumor differentiation,infiltration depth,lymphnode metastasis,angiogenesis and TNM staging of hepatic cell carcinoma

Effects of Baicalin on CYP3A4,CYP2C9 and CYP2C19 expressions in Chang Liver cells

Abstract: 【Objective】To find out the effects of Baicalin on CYP3A4, CYP2C9 and CYP2C19 expressions in Chang Liver cells. 【Methods】mRNA expressions of CYP3A4, CYP2C9 and CYP2C19 were determined by using RT-PCR in Chang Liver cells when incubated with DMSO 0.1% (v/v) (solvent control) and Baicalin (0.01 μM, 0.1 μM and 1 μM ) for 24, 36 and 48 hours, respectively. 【Results】CYP3A4, CYP2C9 and CYP2C19 expressions tended to increase when treated with different concentrations of baicalin in Chang Liver cells for 24 and 36 hours, respectively, and CYP3A4 and CYP2C9 expressions significantly decreased when incubated with different concentrations of baicalin for 48 hours (P0.001).【Conclusion】Baicalin had significant effects on CYP3A4, CYP2C19 and CYP2C9 expressions in a biphasic manner of first induction and later inhibition, and the effects varied with different concentrations of baicalin and incubated periods.

Effects of Kanamycin on auditory brainstem response and distortion product otoacoustic emission in Kunming mice

Abstract: Objective To investigate the effects of Kanamycin(KM) on auditory brainstem responses(ABR) and distortion product otoacoustic emission(DPOAE) in Kunming mice, and to explore the susceptibility of aminoglycoside antibiotics(AmAn) induced ototoxicity in Kunming mice.Methods 16 male Kunming mice were randomly divided into KM group and control group.Mice were injected subcutaneously with KM(2×750 mg/kg·day) or equal physiological saline for 2 weeks.Auditory thresholds and DPOAE amplitudes were recorded respectively before and after injections.Morphological changes of the cochlea were observed by silver nitrate staining.Results After treatment with KM for 1 week, DPOAE amplitudes were dramatically decreased, while auditory threshold shifts were not obvious changed.Threshold shifts were significantly elevated after 2 weeks of injections, and increased further after cessation of treatment.There were no remarkable changes of auditory threshold shifts and DPOAE amplitudes in the control group.The silver nitrate staining of cochlea showed an orderly outline of outer hair cells in control group, while KM-treated animals exhibited severe to subtotal outer hair cells loss.Conclusion Kunming mouse was susceptible to AmAn, and a Kunming mouse model of AmAn induced ototoxicity could be established by administration of KM.DPOAE might be more sensitive than ABR in auditory function detection.

Effect of acarbose on coronary atherosclerotic heart disease complicating impaired glucose tolerance

Abstract: 【Objective】To explore the effect of acarbose on coronary atherosclerotic heart disease complicating impaired glucose tolerance 【Methods】187 case patients with coronary atherosclerotic heart disease complicating impaired glucose tolerance were divided into two groups, test group 97 case was treated with acarbose, and other treatments were as same as the control group, to survey the BG levels in blood and new occurred cardiovascular event 【Results】Of 187 cases, 6 patients were dead, after treatment, the levels of BMI and TG of two groups were significantly lower than pretherapy In test group, levels of OGTT 2h, HbA1c were significantly lower than pretherapy, incidence of acute myocardial infarction, angina pectoris, total cardiovascular event and diabetes were significantly lower than the control group′s【Conclusions】Acarbose can reduce cardiovascular event and diabetes in patients with coronary atherosclerotic heart disease complicating impaired glucose tolerance

Comparative study on the congenital ventricular septal defect between interventional and surgical procedures

Abstract: 【Objective】Both surgical procedure and interventional procedure are effective treatments for congenital ventricular septal defect. We analyzed them in different aspects to get the best choice for the patient.【Method】 30 patients were treated with surgical procedure. 30 patients were treated with interventional procedure. 【Result】 No complication occured in the surgical group. In the interventional group,1 patient was treated surgically because the shift of the occluder,1 patient was treated surgically because complete AVB. The hospital stay of interventional group was less than the surgical group,while the cost was higher.【Conclusion】Each procedure has its advantages and disadvantages. Doctor should analyze the datas of the patient before he make the choice.

Path analysis on relationship between medical coping modes,social support and quality of life for renal transplant patients

Abstract: 【Objective】 To explore the relationship between medical coping modes,social support and quality of life for renal transplant patients.【Methods】 Through convenient sampling,123 renal transplant patients were evaluated by Medical Coping Modes Questionnaire (MCMQ),Social Support Rating Scale (SSRS) and Quality of Life Scale for Patients of Renal Transplantation (QOL-RT).A path analysis was applied by AMOS (Analysis of Moment Structures) version 7.0.【Results】The mean score of three dimensions of MCMQ for renal transplant patients were:face(20.89±3.60),avoid (17.15±2.73)and surrender(9.50±2.74),respectively.The total mean score of SSRS for renal transplant patients was (42.89±8.48).The total mean score of QOL for renal transplant patients was (128.74±16.29).Path analysis showed that avoid had directly positive effects on QOL (β =0.183,P 0.01),surrender had directly negative effects on QOL (β =-0.388,P 0.01),face had directly positive effects on social support (β =0.232,P 0.01),surrender had directly negative effects on social(β =-0.203,P 0.01),and social support had directly positive effects on QOL (β =0.428,P 0.01).Medical coping modes had indirect effects on QOL through social support path.【Conclusion】Medical coping modes has direct effects on QOL for renal transplant patients.Medical coping modes has indirect effects on QOL for renal transplant patients.Social support as a mediated variable mediates the relationship between Medical coping modes and QOL for renal transplant patients.

Clinical analysis of adult type pulmonary blastoma

Abstract: 【Objective】To study the clinical characteristics, pathological features, diagnosis, therapy and prognosis of adult type pulmonary blastoma. 【Methods】The clinical data of 10 cases with adult type pulmonary blastoma pathologically comfirmed were retrospectively analyzed. 【Results】Chest pain (6 cases) and cough with bloody sputum (4 cases) and shortness of breath (3 cases) were the main clinical features. The isolated solid tumor surrounding lung extended to the pleura can be seen in lung CT. The final diagnosis depends on pathological examination by surgical resection or percutaneous lung biopsy. Surgical resection were given in 9 cases and chemotherapy were given in 5 cases. The median survival time was 39 months in this group. 【Conclusion】Due to unspecific symptoms, adult type pulmonary blastoma should be diagnosed and treated with surgical resection and chemotherapy as early as possible.

Application of patients′ role-playing in the teaching of diagnostics history inquire

Abstract: 【Objective】To investigate the effect of patients′ role-playing in the teaching of diagnostics history inquiry compared with the traditional methods.【Method】Based on the traditional teaching methods, patients′ roleplaying teaching methods were performed in the experimental group, which included teachers-patients role-playing and students-patients role-playing. Finally the results of the both groups′ tests were got.【Results】The achievements of chief complain and present history in the experimental group were significant better than the control group (P 0.05). 【Conclusions】The application of patients′ role-playing is valuable for the teaching of diagnostics history inquiry.

Investigation and analysis of risk factors on subhealth status of middle-aged teachers in colleges in shenzhen and Guangzhou city

Abstract: 【Objectives】To investigate the subhealth status of middle-aged teachers in colleges in Shenzhen and Guangzhou,to study its symptoms of subhealthy state and analyse the influencing factors of subhealth state, to provide scientific proof for health manage of them. 【Methods】515 45-59 year-old middle-aged teachers in ten colleges in Guangzhou and Shenzhen were investigated with the health questionaires and collected relevant information had statistical analysis. 【Results】65% of middle-aged college teachers are in subhealth status, The risk age was between 45 and 55. The higher in educational level, the lower the prevalence rate of subhealth incidence. The prevalence rate in women middle-aged teachers was higher than men teachers. The mainly symptoms of subhealthy state are chronic fatigue syndrome, neck and shoulder syndrome, easily agitated, upset, dizziness, decreased energy and so on. work pressure, family pressure, personal habits, lack of exercise are the risk factors of subhealth status. 【Conclusions】We should pay more attention on subhealth staus of the middle-aged teachers in colleges ,carry a health assessment and management, develop health management programs on diet, living habits, psychological, sports, etc. and guide the healthy life of middle-aged teachers in colleges.

Effect of MK-801 on extracellular levels of glutamate in the hippocampal dentate gyrus during learning-dependent LTP in free-moving conscious rats

Abstract: 【Objective】To investigate the neurochemical mechanisms responsible for learning dependent longterm potentiation(LTP),the effect of MK-801 on extracellular levels of Glutamate(Glu) was measured in hippocampal dentate gyrus(DG) region during the period of establishment and extinction of the conditioned reflex in freemoving conscious rats.【Methods】The concentration of glutamate(Glu) was measured by microdialysis and highperformance liquid chromatography(HPLC) methods and MK-801 was administrated by local-microinjection.The population spike(PS),a parameter of LTP,and conditioned reflex were examined by implanted electrodes and combining electrophysiological-behavioral methods.【Results】The extracellular levels of Glu in the DG region were significantly reduced by local-microinjection of MK-801(60 nmol/L) without training(P 0.05).In the control group 2 and experiment group 2,the extracellular levels of Glu(P 0.001) in the DG region were significantly increased during the establishment of conditioned reflex and gradually returned following the extinction of conditioned reflex.The changes of Glu were correlated to the change of PS and both changes roughly follow the correct rate of behavior.Nevertheless,the changes of Glu and PS were significantly attenuated by local-microinjection of MK-801,as well as the establishment of conditional response was significantly inhibited by MK-801.【Conclusion】NMDA receptor participates in the Glu-associated learning-dependent LTP in hippocampus DG region,it may be partly dependent on modulation of presynaptic release of Glu.

Clinical analysis of 34 cases of pulmonary tuberculosis complicated with diabetes mellitus

Abstract: Objectives To investigate the clinical characteristics, therapeutic regimen and outcome of pulmonary tuberculosis complicated with DM(diabetes mellitus) and effect between pulmonary tuberculosis and diabetes mellitus.Methods The clinical features, diagnosis, treatment and outcome of 34 cases of pulmonary tuberculosis complicated with DM were analyzed. Results In two diseases ,the patients who suffer from DM first are 20 cases, suffer from pulmonary tuberculosis first are 5 cases, suffer from two diseases at the same time are 9 cases. Pulmonary tuberculosis complicated with diabetes mellitus much is type of pulmonary tuberculosis Ⅲ (82.4%). The property of the pathological changes is mainly the caseous exudation. Between which controlled ideally and which control badly, the differences of the marked tuberculosis foci absorption are remarkable (P 0.05). Conclusions The diabetes mellitus and pulmonary tuberculosis affect mutually, and the diabetes mellitus affects pulmonary tuberculosis more greatly. Early diagnosis and a good control of the diabetes mellitus, and the persistence of DOTS program of anti-tuberculosis treatments are the keys that to obtain the good prognosis.

Expression of folate receptor in the tissues and cells of nasopharyngeal carcinoma

Abstract: 【Objective】 In order to investigate the expression of folate receptor in nasopharyngeal carcinoma(NPC) and its correlation with clinical and pathological features.【Methods】Using immunohistochemistry and im-munocytofluorescense detect the expression of folate receptor in 20 NPC specimens and six cell lines such as HNE1,HNE2,CNE1,CNE2,SUNE1,5-8F,the relationship between the expression of FR and clinicopathological features of specimens was analyzed.【Results】The positive expression of folate receptor was detected in 85%(17/20) NPC tissue and negative in normal nasopharyngeal tissue(0/8) which did not correlate with gender,age,pathology type,but correlate with clinical stage(P =0.045).In six cell lines folate receptor positively expression was found only in HNE1.【Conclusions】The results suggested that over express folate receptor is presented in most of NPC tissues,which provided basement to create molecular drug targeting folate receptor on NPC cells.

Study of the correlation between the variation at -30 position of glucokinase gene promoter and type 2 diabetes

Abstract: 【Objective】 To investigate the association between the mutation at position-30 of the glucokinase gene promoter and type 2 diabetes and some relative index.【Methods】 389 unrelated subjects were studied by polymerase chain reaction-restriction fragment length polymorphism assay.【Result】Genotype frequency of GG,GA and AA was 63.1%,31.9% and 5% in type 2 diabetes subjects and 66.4%,31.8% and 1.8% in normal control sub-jects.【Conclusion】The variant at position-30 of the GCK gene promoter would not play a major role in the onset of type 2 diabetes,the onset year of type 2 diabetes would be earlier and the obesity would be not accompanied when the two alley gene exist in the same body.

Preparation and in vitro release of Docetaxel loaded PLGA/nHA microspheres

Abstract: 【Objective】sTo prepare docetaxel(DTX) loaded PLGA/nHA microspheres, research the effect of nHA on drug loading, encapsulation efficiency, in vitro of composite microspheres and inhibiting the growth of prostate cancers.MethodPLGA/nHA-DTX composite microspheres were prepared by a single-emulsion solvent evaporation method(S/O/W), with hydrophobic anticancer docetaxel as model drug.nHA and drug-loaded nHA were analyzed by TEM and FTIR, and drug loading, encapsulation efficiency, size and in vitro release of microspheres were studied with SEM, laser particle size analyzer and HPLC, etc.【Results】The results show that nHA have a strong adsorption with DTX.Drug loading and encapsulation efficiency of PLGA/nHA-DTX composite microspheres were 3.92% and 88.7%, and have greatly improved compared to neat PLGA-DTX microspheres.After initial burst, composite microspheres released more slowly than neat PLGA microspheres.At the 30d after in vitro release, the cumulative release rate of composite microspheres and neat PLGA microspheres were respectively 62.40% and 72.70%.Cytotoxicity test results showed that PLGA/nHA composite microspheres had better effect than PLGA microspheres andDTX to inhibit cancer cell growth.Conclusion Because nHA has a strong adsorption with DTX, PLGA/nHADTX composite microspheres compared to neat PLGA-DTX microspheres enhance drug loading and encapsulation efficiency, and have better drug delivery effect.The effect of inhibiting the growth of cancers of the composite microspheres is also better.

Application of MSCT scanning technique on the diagnosis of infant tracheal and bronchial nonmetal foreign body

Abstract: 【Objective】To assess the application and significance of MSCT(multislice spiral CT) scanning technique on the diagnosis of infant tracheal and bronchial nonmetal foreign body.【Methods】32 cases of infants with suspected tracheal and bronchial nonmetal foreign body were scanned with thin-slice collimations by MSCT,and the postprocessing of MPR(multiplanar reformation),cMPR(curve MPR),CTVE(virtual endoscopy) were made by using the volume data.The foreign body were extracted by bronchial endoscopy after the diagnosis were definite.【Results】 32 infants with tracheal and bronchial nonmetal foreign body included 6 cases in trachia,12 in right main bronchia,1 in middle bronchia,8 in right inferior bronchia,4 in left main bronchia,1 in left inferior bronchia.The CT diagnosis were in accordance with that of bronchial endoscopy.【Conclusions】The direct and indirect signs of foreign body were all displayed on MSCT thin-slice and postprocessing imagings.MSCT is the first choice method for diagnosing tracheal and bronchial nonmetal foreign body.

Expression and significance of snai2 in gastric cancer by cDNA microarray

Abstract: 【Objective】We detected the Snai2 mRNA expression in gastric carcinoma and analyzed its biological significance. 【Methods】20 gastric carcinoma tissue samples were used for differentially expressed gene analysis by gene chip while 20 normal gastric mucosa were mixed together as commom reference. Total RNA was extracted with Trizol from 30 gastric carcinoma and mRNA was transcribed reversely into cDNA, the expression of Snai2 was examined by real-time quantitative Polymerase chain reaction (PCR) with Eva -Green staining. 【Results】The gene expression chip showed that almost 100 percent of Snai2 mRNA over expressed in poor differentiated carcinoma tissues than normal and highly differentiated ones, the fluorogenic ratio in poor and moderate also were higher than well ones; Real-time PCR data showed different expression of Snai2 mRNA in gastric carcinoma, there was a tendence, the poorer differentiation, the more expression (P 0.05). 【Conclusions】Snai2 can be a biomarkers of the differentiation in gastric carcinoma, and as an useful tool for judgement of the prognosis of tumor.

Expression and clinical significance of CXCR4 and MMP-9 in bladder cancer

Abstract: 【Objective】To investigate the expression and clinical significance of CXCR4 and MMP-9 in bladder cancer. 【Methods】 Forty bladder cancer specimens and ten normal bladder mucosa tissues were included in this study, respectively as cancer group and control group. The expression of CXCR4 and MMP9 was detected by immuno-histochemistry. The relation between CXCR4 and MMP9 was analyzed, as clinical biological behavior. 【Result】The positive rate of CXCR4 and MMP-9 in cancer group was respectively 77.5% (31/40) and 80% (32/40), respectively significantly higher than that in control group [20% (2/10) and 20% (2/10), P 0.05]. Moreover, The positive rate of CXCR4 and MMP-9 was respectively 100% and 95.5% in the bladder cancer specimens whose infiltrative depth reached muscular layer, respectively significantly higher than that in the bladder cancer specimens whose infiltrative depth limited mucosa layer or submucosa [50% and 61.1%, P 0.05]. There was positive correlation between CXCR4 and MMP-9 in cancer group. 【Conclusions】Up-regulated expression and positive correlation of CXCR4 and MMP-9 was detected in infiltrative bladder cancer, indicating that they may be two indexes of early infiltrative bladder cancer.

Detection of spontaneous hepatitis B virus YMDD motif mutation by fluorescent hybridlzation blprobe PCR and melting curve assay

Abstract: 【Objective】To investigate the natural mutation of hepatitis B virus(HBV) YMDD motif in the patients with chronic hepatitis B(CHB) who were not treated with lamivudine,and to observe its epidemiological characteristic.【Methods】The natural mutation of HBV YMDD motif was detected by fluorescent hybridlzation blprobe PCR and melting curve assay(FH-PCR-MC) in 119 patients with CHB.【Results】Among 119 subjects with CHB,spontaneous YMDD variants were detected in 23 subjects(YVDD,YIDD and YVDD/YIDD for 20,2 and 1 subject,respectively),and were all found to coexist with wild-type virus.YMDD variants account for 40%～50%,30%～40%,20%～30%,20% of total virus load in 4,6,6,7 subjects,respectively.【Conclusions】There are natural mutants of HBV YMDD in the patients with CHB who were not treated with lamivudine.And natural mutants of HBV YMDD were all found to coexist with wild-type virus.

Utility of maternal creatine kinase in the evaluation of ectopic pregnancy

Abstract: 【Objective】To determine the value of creatine kinase in ectopic pregnancy. 【Method】The levels of serum creatine kinase in the 3 groups (intrauterine pregnany,high risk ectopic pregnancy and normal ectopic pregnancy were analysed)【.Results】The level of serum creatine kinase in the ectopic prdgnancy is higher than in the intrauterine pregnany,the level of serum creatine kinase in the high risk ectopic prdgnancy is higher than normal ectopic pregnancy,and the level of serum HCG is in the high risk ectopic prdgnancy is higher than normal ectopic pregnancy.【Conclusion】The level of serum creatine kinase is reference to tubal damage.

Application of laser resonance Raman spectroscopy in gastric cancer

Abstract: 【Objective】 To study laser resonance Raman spectroscopy application in gastric cancer. 【Methods】 The specimens as follows were collected: the serum preoperation, post-operation for three days of gastric cancer patients, nomal controls, the culture medium of SGC7901 gastric cancer cells cultured for 24, 48 and 72 hours and 1640 culture medium. 【Results】At wavelength of 585 nm, 633 nm, 656 nm, 674 nm, 707 nm, 772 nm, 776 nm and 798 nm,the Raman peak of gastric cancer patients was higher than that of the normal (P 0.01). Study of the Raman spectrum of gastric cancer patients preoperation and post-operation for three days shows that the Raman peak of post-operation group was obviously lower than the Raman peak of pre-operation group. The numerical value of the P was 0.05 and the statistical difference is significant. From Raman spectroscopic study of the culture medium, the Raman peak repeatability at 585 nm, 633 nm, 656 nm, 674 nm, 707 nm, 772 nm, 776 nm and 798 nm could be found. But the Raman peaks at 633 nm, 656 nm and 674 nm can only be found in the culture medium of gastric cancer cells. The Raman peak was positively correlated with the culture time. 【Conclusions】 The Raman peak of gastric cancer patients was higher than that of the normal. The Raman peak of post-operation group was obviously lower than the Raman peak of pre-operation group.The technique Raman spectrum can reflect the differences of the spectrums. It is considered effctive for the screening of gastric cancer, the monitoring of prognosis and the judgment of therapeutic effect. The differences of the spectrums between the culture medium of gastric cancer cells and 1640 medium are significant. The Raman spectrum of culture medium of gastric cancer cells rises as time goes by.

Delay of health-seeking and its related factors among outpatients with sexually transmitted disease

Abstract: 【Objective】To explore the occurrence of delay of health-seeking and its related factors of sexually transmitted diseases (STD) outpatients. 【Methods】200 consecutive STD outpatients who attended the clinic of a general hospital were investigated using a self-designed questionnaire, which included background information, STD related knowledge and health-seeking behaviors. Delay of health-seeking was defined as delayed seven and more days from the day of onset. 【Results】Among 200 STD outpatients, 130 cases (65.0%) had delay of health-seeking. The median days of health-seeking delay were 24 (P25=8 days, P75=64.5 days). Major factors related to delay of health-seeking were the history of STD, the self-attribution of STD symptoms and the level of knowledge related to STD. 【Conclusion】Delay of health-seeking is common among the STD outpatients. To dissimilate knowledge related to STD among the public, and to modify the care-seeking behavior of STD outpatients are of key importance for controlling and treatment of STD/HIV infection.

Influence of different kind of Dysiipidaemia on complication in type 2 diabetic patients

Abstract: 【Objective】To evaluate the effect of different of Dysiipidaemia on complication in type 2 diabetic patients 【Methods】91 type 2 diabetic patients were divided into normal and Dysiipidaemic groups according to ATPIII standard The Dysiipidaemic group included 3 subgroup as follows: high TG group with high triglycerides, high TC group with high cholesterol, mix group with both high triglycerides and high cholesterol Homeostasis model assessment was applied to estimate pancreatic islet B cell function Examine blood pressure, trace of albumin all of 24 hours urine, ECG, type B ultrasound, EMG and eyegroud in order to evaluate the complication 【Results】Incidence of Dysiipidaemia among these type 2 diabetic patients is 54% Fasting serum C peptide, FINS are increased and incidence of fatty liver, coronary heart disease ,high bood pressure is growing in the high TG group Lp (a) is advancing in TC group, abnormal high TC and ly (a) were connected with the disturbance of nerve and cataract or DN and DR tighty Common clinical character such as BMI was higher in high TG group than normal group 【Conclusion】 High trglycerides was most common in type 2 diabetic paients, TG played the main role in fatty liver and principal blood vessels disease such as coronary heart disease ,high bood pressure However, there was close relationship between TC, LP(a) and tiny blood vessel diseases such as DN

Effect of tetrandrine on proliferation and apoptosis of human cervical cancer cell line hela

Abstract: 【Objective】To investigate the anti-tumor effect of tetrandrine on human cervical cancer cell line Hela in vitro. Effects of tetrandrine on proliferation and apoptosis of human ovarian cancer cell Hela were observed.【Methods】 The effects of tetrandrine on proliferation of Hela cells was observed by MTT assay and clonogenic assay. Apoptosis was observed by acridine orange(AO)/ethidium bromide(EB) Fluorescent staining,DNA gel electrophoresis and flow cytometry,and the expression of apoptosis related gene was analyzed by immunohistochemical staining method. 【Result】Tetrandrine inhibits the proliferation of Hela cells in a dosage dependent manner and induces apoptosis. Immunohistochemical staining showed that the expression of Bcl-2,decreasing and the expression of Bax increasing in Hela cells treated with tetrandrine.【Conclusion】Tetrandrine inhibits the proliferation of Hela in the dose dependent manner,and induces apoptosis. The antitumor effect of tetrandrine may be due to the regulation of the expressions of Bcl-2 and Bax.