Showing papers in "European Journal of Cell Biology in 1987"

TL;DR: It is demonstrated that the gap junction 26 kDa protein can be posttranslationally modified by cAMP-dependent phosphorylation and in extracts of rat hepatocarcinoma MH1C1 cells and dog kidney MDCK cells, a phosphorylated 26 k da protein can been immunoprecipitated using anti-liver 26 k Da.

TL;DR: The ultrastructural cytochemical localization of xanthine oxidase activity in rat liver was investigated by the cerium technique and high levels of molybdenum were found in isolated peroxisomal cores by atomic absorption spectroscopy, thus corroborating the association of the molyBdenum-containing enzyme with the cores.

TL;DR: Presentation d'un modele d'organisation labile des filaments d'actine au niveau de l'extremite d'hyphes en croissance du champignon S. f.

TL;DR: A standardized four-step assay for the flow cytometric determination of the oxidative activity of human polymorphonuclear leukocytes (PMNL) from normal human individuals and from septic patients was developed, using 2,7-dichlorofluorescin-diacetate (DCFH-DA) as indicator for the intracellular formation of H2O2 and free radicals.

TL;DR: Treatment of CEC with botulinum C2 toxin induced a time-dependent disorganization of the typical architecture of the microfilament network as shown by fluorescein-phalloidin staining.

TL;DR: A monoclonal antibody is described which specifically reacts with DNA both in double and single-stranded forms but not with other molecules and structures, including deoxyribonucleotides and RNAs, which is used in immunocytochemical experiments on tissue sections and permeabilized cultured cells.

TL;DR: In yeasts proliferation of microbodies (glyoxysomes/peroxisomes) is largely prescribed by the growth environment and due to the fact that the organelles harbour essential enzymic functions required for the metabolism of the carbon and/or nitrogen source.

TL;DR: It is concluded that a 30 and a 33 kDa polypeptide make part of the lateral elements of synaptonemal complexes of the rat.

TL;DR: Linkage of integral membrane proteins via ankyrin to the spectrin-based membrane cytoskeleton may provide a molecular basis for restricting the lateral mobility of certain membrane proteins and localizing them in a nonrandom or polarized fashion at specialized domains of the plasma membrane.

TL;DR: It is suggested that pH-independent sorting mechanisms contribute to the targeting of cathepsin D in U937 cells and higher concentrations of the drugs can arrest the intracellular transport completely.

TL;DR: The results suggest that the accumulation of calmodulin at the apical end of merozoites plays an important role during their attachment to and/or invasion of the host erythrocyte, possibly through activation of Ca2+ dependent processes.

TL;DR: The results suggest that different growth conditions used in different laboratories may explain some but not all of the discrepancies in the literature on mitotic keratin filament disruption.

TL;DR: The nuclear specificity shown by these antibodies further demonstrates the antigenicity of proteins related to intermediate filament proteins in patients with autoimmune disorders.

TL;DR: It is concluded that plakoglobin occurs, at least in lens cells, throughout the entire subplasmalemmal coat, coexisting in this situation not only with vinculin but also with spectrin and 4.1 protein(s).

TL;DR: In this article, the kidney analog of the erythrocyte anion exchanger, band 3, served as the first example of an anion translocating membrane protein in a nucleated cell type to be localized at the ultrastructural level.

TL;DR: The results suggest that the association of intermediate filaments with lipid vesicles is initiated by interaction of the arginine-rich N-termini of their subunit proteins with the negatively charged vesicle surface and stabilized by partial insertion of the protein molecules into the lipid bilayer, particularly at those sites where immiscible, nonpolar lipids create defects in phospholipid packing.

TL;DR: If budding is inhibited, inhibition must occur at all stages of the budding process, which can be partly, if not entirely, explained by their greater size.

TL;DR: The overt circadian rhythm in a wildtype (wt+) and a short period (s-) strain of Chlamydomonas reinhardii has been studied in space using the photoaccumulation behavior as the recorded parameter.

TL;DR: It is proposed that the regulation of the biosynthetic activity of adherent cells contracting the lattice is related to mechanical information resulting from the interactions between the cells and their support.

TL;DR: It is shown that departures from the "one antigen, one gold" ideal are systematic, so that an efficiency can be calculated and quantitative results can be obtained.

TL;DR: Human platelets stimulated with thrombin or collagen in order to induce the release of alpha-granules and dense bodies suggest that swelling of the secretory organelles plays an important role for granule fusion and platelet exocytosis.

TL;DR: The association of myOSin with migrating basal bodies suggests that myosin could be involved in basal body movements.

TL;DR: Observations are compatible with the view that newly synthesized aminopeptidase N is delivered directly to the microvillar membrane by smooth vesicles having a diameter about 70 to 100 nm and does not pass the basolateral membrane on its way to the brush border membrane.

TL;DR: The experiments demonstrate that cytokeratins can become differentially distributed within the cytoplasm after microinjection of an anti-cytokeratin monoclonal antibody, and suggest that different members of one cytokeratin subfamily may be associated with cytokersatin filaments which have different functions within the same cell.

TL;DR: Observations are consistent with the concept that new peroxisomes are formed from preexisting ones by budding and segmentation, while catalase is transferred uniformly to all new segments, uricase is compartmentalized in certain portions, of the apparently growing "peroxisomal reticulum".

TL;DR: Information about the pattern of proteoglycans in normal human arterial tissue should constitute a useful basis for evaluating perturbations in proteoglycan distribution in arteriosclerotic plaques.

TL;DR: In tissue O2 is rapidly depleted both by respiration and the chemical demands of the glutaraldehyde-amine reactions during the cross-linking process, consistent with the synthesis of pyridine derivatives from glutarhyde-amine precursors in which the last step is an irreversible oxidation of dihydropyridine to pyridines.