Showing papers in "European Journal of Cell Biology in 1992"

TL;DR: Results indicate that, in addition to alpha and beta' (class III)-tubulin, other beta-tubulin isotypes are also glutamylated, and this antibody has been used to analyze the cell and tissue distributions of glutamelated tubulin.

TL;DR: By in situ hybridization expression of the B2t chain was localized to specific epithelial cells in skin, lung, and kidney as opposed to a general epithelial and endothelial cellexpression of the laminin B2 chain in the same tissues.

TL;DR: It was found that lowering the concentration of extracellular Ca2+, which reduced cell contact, did not affect the abundance, the state of phosphorylation, or the TPA-inducedosphorylation of Cx43, and second messenger pathways involving protein kinase C, but not cAMP- or cGMP-dependentprotein kinase, led to changes in electrophoretic mobility of C x43.

TL;DR: Data support the antiadhesive role of decorin regardless of whether subsequent cell spreading is supported or not, and it is concluded that several structural features of thrombospondin and of decorations contribute to the mutual interaction of the two macromolecules.

TL;DR: Among the members of the cytokeratin (CK) subfamily of intermediate filament (IF) proteins, CK 17 is remarkable as it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelias.

TL;DR: Characterization of mouse connexin31 cDNA coding for a protein of 270 amino acids that shows 8 amino acid exchanges compared to its rat analog recently deduced from its genomic sequence is described.

TL;DR: Plectin is established as a novel component of focal contact complexes and suggest that plectin plays a role as mediator between intermediate filaments and actin filaments.

TL;DR: It is shown here that SP-A enhances the phagocytosis of some non-opsonized bacteria: Escherichia coli growing logarithmically (E. coli/log), Pseudomonas aeruginosa/log as well as from stationary phase (P. aerug inosa/stat) and Staphylococcus aureus/log.

TL;DR: The sequence comparison of mouse Cx26 and Cx32 promoter regions provides hints for possible consensus elements that could control individual expression as well as common regulation of these gap junction genes in various tissues.

TL;DR: The results of studies indicate that TGN38 returns from the plasma membrane via the endocytic pathway, indicating that different molecules control membrane traffic from the Golgi cisternae and from the TGN.

TL;DR: It is concluded that ECP inhibits proteoglycan degradation in fibroblasts, which indicates a role for the eosinophil in generation of fibrosis.

TL;DR: Immunocytochemical, freeze fracture, label fracture and electron microscopic observations have shown that the amphipathic peptide generates perturbations of the lipid bilayer leading to altered permeability of the surface membrane and death of the parasite.

TL;DR: The persistent presence of U3 RNA and fibrillarin after pre-rRNA depletion suggests that DFC could represent an anchorage site for U3 snRNPs, before their entering another cycle of pre- rRNA processing reactions.

TL;DR: It is found that the distribution of the enzymes corresponds with diverse forms of actin cytoskeletal organization of these cells, and the codistribution is maintained in cells treated with cytochalasin D.

TL;DR: A monoclonal antibody directed against rat liver heat shock protein M(r) 90,000 (hsp90) was produced and a major part of hsp90 immunoreactivity was diffusely distributed throughout the interphase cytoplasm, but a weak nuclear staining with non-stained nucleoli was also present, however, only detectable after methanol and not after formaldehyde/Triton X-100 fixation.

TL;DR: It is demonstrated that tail-less vimentin, isolated from transfected bacteria (Escherichia coli), when used for assembly in vitro, forms normal-looking, loosely packed IFs and that spheroidal aggregate structures formed from the tail-deleted vimentalin, were seen not only in the cytoplasm but also in the nucleus, indicating a role of the tail in higher order organization and compartmentalization of the vimentsin IF system.

TL;DR: This procedure, that can be extended--in principle--to any vascular bed, obviates the use of cultured cells for studying the biochemistry of the endothelium, at least as far as the luminal endothelial plasmalemma is concerned.

TL;DR: Data are consistent with, and allow the generalization of, the concept that the BFA-induced block of anterograde membrane traffic results from the inhibition of vesicle formation from a donor compartment.

TL;DR: A perfusion apparatus with various culture containers and compatible cell holder sets was constructed which allows the simulation of organotypic conditions and exhibited an excellent morphological appearance showing typical features of principal and intercalated cells.

TL;DR: measurements with fura-2-dextran in chemotactically active D. discoideum amoebae revealed that the large decrease in the extracellular calcium concentration is not accompanied by an overall change in [Ca++]i.

TL;DR: Evidence is presented that AR42J cells are amphicrine in that they contain zymogen granules as well as small neuroendocrine vesicles and typical neurotransmitters and may be indicative of a derivation from a cell with multipotential character.

TL;DR: This study shows that IFN-gamma regulates cell behavior in three-dimensional collagen matrices and decreases protein and specifically glycosaminoglycan synthesis in scleroderma fibroblasts, and modulates the interactions between cells and matrix that lead to the retraction of the lattice.

TL;DR: The results indicate that BFA rapidly inhibits secretion and causes dismantling of the Golgi stacks in pancreatic acinar cells, but clusters of vesicles consisting of bona fide Golgi remnants persist even with prolonged exposure to BFA, indicating that in the exocrine pancreas there is a dissociation of BFA's effects on the exocytic pathway.

TL;DR: It was shown that the level of MAP 1B mRNA increased dramatically upon nerve growth factor-induced PC12 cell differentiation and it was concluded that single proteolytically unprocessedMAP 1B molecules would have the potential to function as microtubule cross-linkers.

TL;DR: The data suggest that, unlike the situation in mammalian cells where most transferrin is recycled to the cell surface, in African trypanosomes transferrin was routed into lysosomes and may not, therefore, be recycled.

TL;DR: New evidence is provided suggesting that the cellular mechanism utilized by postnatal gonocytes in relocating to the basement membrane are those mediating active migration, and both the Golgi complex and microtubules are involved in development of processes by gonocytes.

TL;DR: The essential yeast nuclear pore protein NSP1 was placed under the control of the regulatable GAL10 promoter and targeted to the nuclear envelope, the nuclear por density increases, and nuclear accumulation of reporter proteins is restored.

TL;DR: Human myeloid leukemia cells (i.e., HL-60, U937, THP-1) which are induced to differentiate along the monocytic pathway by 12-O-tetradecanoylphorbol-13-acetate (TPA), revert back to the undifferentiated phenotype after 3 to 4 weeks, and down-regulation of markers associated withmonocytic differentiation in combination with metabolic changes restore the original cell phenotype.

TL;DR: It is concluded that pancreatic adenocarcinoma cells secrete hyaluronan and thus contribute to the extracellular matrix of the tumor tissue and in pancreatic carcinoma cells regulation of HA biosynthesis seems not to be positively correlated to proliferation as has been demonstrated for fibroblasts.