Showing papers in "Experimental Cell Research in 1959"

TL;DR: The survival of brain, liver, thymus, spleen, bone marrow, testis and pancreas was unsatisfactory and partial survival was obtained in the case of kidney, adrenal and spinal ganglion.

TL;DR: Formol-calcium fixation followed by treatment with hypertonic sucrose containing gum acacia (“gum-sucrose”) gives the best preservation of the acid phosphatase integrity of rat kidney and liver cells.

TL;DR: Rat peritoneal mast cells were isolated by differential centrifugation in Ficoll and retained their histamine in spite of repeated washing and subsequent incubation at 37 °C, and reacted by disrupting to agents like compound 48 80 and antigen.

TL;DR: A method is described for dissociating the heart ventricles and skeletal muscle of 12- to 14-day-old chick embryos and for cultivating quantitatively the free cells thus obtained and some morphological and functional aspects relevant to the development of the culture methods are discussed.

TL;DR: The material elaborated by odontoblasts and osteoblasts is believed to be a collagen or collagen precursor which is secreted outside the cell to become the collagen fibrils of dentin and bone respectively.

TL;DR: Although populations of human cervical carcinoma cells (strain HeLa) may be induced to undergo parasynchronous division by chilling of the cell culture, the time of cell division is restricted to a very small portion of the total growth cycle.

TL;DR: Results from a parallel morphological study by electron microscopy give convincing evidence for a firm association between cholinesterase and membrane structures and for the location of ribonucleic acid in the small granules.

TL;DR: TCA-soluble SH groups were found to fluctuate in correlation with the cell division as in Rapkine's work, and after the removal of the protein, changes in the amounts of SH groups in relation to cell division disappear.

TL;DR: Some theories are elaborated relating the role of ribonucleic acid to protein synthesis, and implicating the structural organization of the cell to the biochemical mechanism of protein synthesis.

TL;DR: The generation times for individual Tetrahymena are highly variable, ranging from 74 to 135 per cent of the average generation time, with variations due to inequalities between cells arising at division.

TL;DR: It is concluded that intracellular hydrolases are only exceptionally involved in synthetic or transfer reactions and that most of them catalyse true hydrolytic processes.

TL;DR: It is probable that compounds are accumulated in bacteria which function primarily as energy reserves, and the majority of these may also act as carbon reserves and one, polyphosphate, as a phosphate reserve.

TL;DR: The increased DNA in nuclei deep in the wall indicates that the doubling of DNA that precedes cell division takes place in the depth of the wall at some distance from the central canal.

TL;DR: It was found that the rate of glucose uptake and lactate production was most rapid during the first part of the logarithmic growth period, but it was found, however, that lactate was taken up by the cells when glucose was still present in relatively high concentrations.

TL;DR: It is concluded that differences in the relative importance of the nucleus and microsomes in limiting growth might be involved and that the relations between growth and division rate under various conditions are adaptive responses developed through evolutionary selection.

TL;DR: In this paper, the effects of serum on the adhesion of tissue cells to glass in culture, are described and the results are discussed in terms of cell-membrane structure and the composition of culture media.

TL;DR: A parallel chemical investigation on standard preparation of microsomes from brain tissue proved the particles to be associated with RNA and the membranes with cholinesterase activity.

TL;DR: Investigations of pairs of sister animals show that the macronucleus varies in size, probably through irregularities in division, and some departures from this hypothesis suggest that the magnitude of the Feulgen reaction may vary somewhat because of the state rather than the amount of DNA.

TL;DR: The alveoli of bat lungs prepared by freezing and drying are lined by a probably complete, homogeneous, non-cellular layer, staining intensely with platinum, and giving a positive reaction with the PAS stain.

TL;DR: Although the sex vesicle disappears at the end of diplotene, the X and the Y remain firmly in end-to-end association until first metaphase, thus assuring the “prereduction” of the X or the Y.

TL;DR: The results indicate that cycles of increased proteolysis form essential steps during certain stages of cell growth preparatory to cell division, and the idea is advanced that the proteinases and peptidases may be operative in the intracellular reactions aiming at the replenishment of the metabolic amino acid pool necessary for protein synthesis.

TL;DR: As detachment from glass is accomplished through rupture occurring within the peripheral zone (“surface”) of cells, it is concluded that environmental factors can act upon the cell and cause a diminution in the cohesive strength of this zone.

TL;DR: A fractionation procedure for the subcellular components of the apical portion of the pea seedlings emphasizes isolation of microsomal particles and separation of nuclei from cytoplasmic components.

TL;DR: Studies are described which suggest that the material of these isolated cilia is essentially homogeneous, and the material appears to contain small amounts of adenine and uracil nucleotides.