Showing papers in "Immunochemistry in 1975"

TL;DR: Intensive research in the author's laboratory over an 11-yr period has now completed the first mapping out of all the antigenic reactive regions of a native protein.

TL;DR: 6-Aminohexyl-Sepharose 4B can be activated with glutaraldehyde to give a stable intermediate Sepharose4B derivative which after removal of excess glutarhyde, covalently binds proteins during a second step, thereby furnishing Separose-protein conjugates of high protein content.

TL;DR: Results are interpreted to indicate that activation of a basophil is due to bridging of two molecules on the cell surface with the resultant formation of a dimer, and the evidence suggests that bridging does not occur withn the two Fab portions of a single IgE molecule.

TL;DR: Using different estrogen derivatives to prepare the immunogen and the estrogen-enzyme conjugate, more sensitive assays could be obtained, with little adverse effect on the specificity.

TL;DR: Rat immunoglobulin A, M, G 2a and G 2b and anti-rat α, μ, and γ were purified by ion exchange and immunoadsorbent chromatography and employed in the quantitation of the principal Immunoglobulins in serum and saliva of young rats from birth until 45 days of age and in milk, saliva and serum of adult rats.

TL;DR: The partial purification of these enzymes indicated that fractions rich in the C3b inactivator bring about destruction of cell bound C4b but another enzyme is necessary for generation of the C4d fragment from C4B.

TL;DR: Fraction B Clq was found to be more soluble in low ionic strength buffers and to have an increased hemolytic activity and the anionic nature of fraction A was demonstrated by electrophoresis in agarose.

TL;DR: Independent evidence for the presence and accurate delineation of this reactive region is presented from studying the immunochemistry of synthetic peptides representing various parts of the region obtained from studies on chemical derivatives of the antive protein.

TL;DR: A mechanism for cyroprecipitation was proposed wherein precipitation in the cold is a direct consequences of binding rather than a property inherent in a nature of the soluble complexes.

TL;DR: A solely radioimmunological method for quantitation of class-specific antibodies to bovine serum albumin (BSA) has been developed and the sensitivity is considerably higher than that of conventional serological quantitations.

TL;DR: In those sera which contained IgE antibodies reacting with wheat most of the activity was found to bind to albumins and globulins, the soluble proteins, with only small amounts binding to gliadin and gluten.

TL;DR: The more limited use of the term ‘lipid A’ and more accurate description of the various lipid-rich endotoxin derivatives is recommended, as Biological activities and molecular ratios of this subtance reveal some similarities between endotoxic glycolipids and this material.

TL;DR: Independent evidence is presented aimed at the accurate delineation of the reactive region by studying the immunochemistry of synthetic peptides corresponding to various parts of the region by synthesizing, exhaustively purified and characterized.

TL;DR: The results indicate that this isotypic λ-chain constant region is the product of a specific gene.

TL;DR: It is suggested that changes in conformation rather than large scale destructive process occur during conversion of C3 into the fragments, and the amino acid composition of all the fragments is in agreement with the composition of C 3.

TL;DR: In this article, it was shown that for the specificite allotypique a1, there exist at most six variants of this specificite a1 and at most five variants of it.

TL;DR: Immunochemical studies of the different preparations of the water-soluble acetylcholine receptor were carried out in order to characterize this receptor and to compare its properties with those of the receptor solubilized in detergents.

TL;DR: A significant internal homology is shown in the highly conserved V H domain, indicating an ancestral gene coding for half a domain in immunoglobulin polypeptide chains.

TL;DR: Batches of bacteriophage T4 were conjugated with one of the haptens: NIP, NP or DNP by inserting an ϵ-aminocaproyl spacer between the haPTen and the phage, resulting in three haptencaproyl phages that were approximately ten times more sensitive to anti-hapten antibodies.

TL;DR: Immunoglobulin E-mediated skin sensitivity to a minor short ragweed pollen allergen, Ra5, was found to be highly significantly associated with the possession of histocompatibility antigen HL-A7 and with antigens of the HL- a7 Cross-reacting group in a study of 139 ragweed-allergic Caucasians.

TL;DR: Free secretory component purified from colostrum had a total carbohydrate content of 18·6 per cent by weight and amino acid composition showed lack of methionine, a relatively low content of the basic amino acids lysine and arginine, and a fairly high content of half cystine.

TL;DR: Results are compatible with the idea that the I determinant-containing part of the SC molecule is responsible for the spontaneous combination of SC with J. chain-containing immunoglobulin polymers.

TL;DR: It was concluded that in early course antisera, the five antigeniv reactive regions of Mb were immunochemically independent and showed no immunochemical interaction.

TL;DR: An attempt to prepare anti-CEA antisera which react only with the cancer specific moiety of CEA determinants was made by the passive administration of an anti-cephalic antiserum which reacted equally with tumor extracts and the faecal extract.

TL;DR: It is deduced from what is known of surfactant mechanisms that DOC solubilizes both ‘integral’ and ‘extrinsic’ membrane proteins, whereas the ionic strength acts primarily on the latter.

TL;DR: 19S IgM anti-IgG immunoglobulin rheumatoid factors isolated from the sera of two patients with rheumatic arthritis were reacted with 7S myeloma IgG globulins of four subclasses in a direct non precipitating reaction.

TL;DR: It is suggested that ovine colostral IgG2 is locally synthesized in mammary gland, wherease colostRAL IgG1 might be a mixed population of molecules local synthesized and transferred from the serum.

TL;DR: It appears that previous workers who investigated this system and reported that anti-TMV antibodies are homogeneous were misled because they assumed an incorrect antigenic valence and used equations that are unsuitable for antigens with a very large valence.

TL;DR: It appears that the mode and time of immunization can be chosen so as to obtain either low affinity and heterogeneous or high affinity and uniformly-binding, antihapten antibody.