Showing papers in "Immunology Letters in 1982"

TL;DR: A simple micromethod for testing human blood leukocyte phagocytosis employing synthetic hydrophilic particles based on 2-hydroxyethylmethacrylate is described and was found suitable for routine testing in both clinical and laboratory practice.

TL;DR: A new nitrocellulose immunoprint technique has been developed to detect specific antigens or/and allergens present among a heterogeneous solution such as a water-soluble crude extract of a grass pollen.

TL;DR: Results provide evidence to substantiate an obligatory role of macrophages in inducing the humoral response to liposome-associated protein antigens.

TL;DR: The ELISA system was sufficiently sensitive to detect thyroglobulin antibody synthesis in mitogen-free lymphocyte cultures from 4 out of the 10 Hashimoto patients and consequently it should be possible to use this system to study the effect of various pathophysiological factors on autoantibody synthesis which would otherwise be masked by mitogenic stimulation.

TL;DR: The somatic cell hybridization technique established 12 clones of mouse hybridoma from MRL/Mp-lpr/lpr(MRL/1) and NZB X NZW(B/W F1) mice, which produce antibodies reacting mainly with ssDNA (anti-ssDNA) and 2 clones from B/W f1 mice,Which had polyspecific reactivity to a wide range of polynucleotides, including synthetic RNA.

TL;DR: Factors indicate that I-J positive non-T-adherent cells play an essential role in the induction of suppressor cells in DTH, as indicated by the induced in vitro induction of antigen-specific suppressor T-cells.

TL;DR: Diazepam, a benzodiazepine derivative useful in the treatment of anxiety disorders, was found to depress both primary antibody to sheep red blood cells and delayed-type hypersensitivity responses in normal mice.

TL;DR: Results gave further conclusive evidence that gene(s) inside HLA region influence either the androgen hormone metabolism itself or cellular sensitivity to hormonal action as it has been presented for congenital adrenal hyperplasia.

TL;DR: It has been established that interferons contain about 60-70% of alpha-helices and not more than 10-20% of beta-structure, while the hydrophobic clusters on the surfaces of the alpha-helical segments were determined.

TL;DR: This study demonstrates both quantitative and qualitative deficiency of suppressor T-cells in at least some patients with insulin-dependent diabetes, that might play an important role in the pathogenesis and autoimmune manifestations of a proportion of patients with diabetes mellitus.

TL;DR: The monoclonal antibody was shown to bind specifically to thymic reticulo-epithelial cells, using an indirect immunofluorescence technique, and once injected into normal mice, the antibody induced the disappearance of FTS from the serum and modified the azathioprine sensitivity of spleen rosette-forming cells for more than 3 weeks.

TL;DR: Five stable hybridoma lines were prepared using the myeloma cell line P3-X63-Ag and shown by quantitative enzyme-immunoassay to be specific for pig transferrin: no cross-reaction was obtained with mouse, human, horse and sheep transferrins.

TL;DR: It is concluded that the observed difference between the protective capacities of IgM and IgG antibodies is due to C-region differences between the mu- and gamma-chains.

TL;DR: Synthetic peptides representative of sequences of the human gamma-chain have been used as potential inhibitors of human [125I]IgG1 binding to human monocytes and mouse macrophages.

TL;DR: Two out of the 3 different streptococcal fractions studied on mice splenocytes were shown to elicit the production of a significant amount of interferon, which was detected in the supernatants from mice activated spleen cells.

TL;DR: The new monoclonal enzyme immunoassay (M-EIA) gave good results for the detection of tumor recurrences in the follow-up of colon carcinoma patients, and detected a similar percentage of slightly elevated CEA values as the conventional assay in patients with non-malignant disease.

TL;DR: Injection of monoclonal IgM antibodies to sheep red blood cells 2 h before immunization with a low dose of antigen (Ag) specifically enhances the direct and indirect plaque-forming cell response.

TL;DR: The advantages of the simultaneous analysis of cytological features and monoclonal antibody-defined surface markers in normal and pathological individual lymphocytes are discussed with regard to lymphoid cell maturation and neoplastic lymphocyte classification.

TL;DR: PGE2 failed to enhance the PC differentiation of lymphocytes from patients with systemic lupus erythematosus (SLE).

TL;DR: The results suggest that CyA suppresses the early stage of the immune response by competing with the function of TAF/LAF on the immune T-cell.

TL;DR: A new immunoelectromicroscopical technique is used that confirms the localization of the 'facteur thymique sérique' (FTS) in the reticulo-epithelial cells of mouse thymus and opens the way to its use for double-immunolabelling.

TL;DR: Two less abundant gangliosides are more potent inhibitors of this antibody, and of the anti-Gd antibodies, than sialosyllactoneotetraosylceramide, and another monoclonal cold agglutinin, Sa, which shares some serological properties with anti- Gd cold aggLutinins, was not inhibited by any of these ganglioides.

TL;DR: The results suggest that regulatory events, not the absence of genetic information, may be responsible for the apparent lack of Ig isotype diversity in this species.

TL;DR: The method of purification of the human placental Fc receptor to an active form is described and the subunits, after removal of dissociating agents, displayed IgG binding activity.

TL;DR: Because several immunoregulatory mechanisms at the level of T-lymphocytes take place in the G1 phase, it can therefore be expected that cytofluorometric analyses of lymphocytes in the various cell cycle phases may improve the interpretation of altered lymphocyte response to lectins and antigens.

TL;DR: The potential of mC3bR to react with C3b-carrying particles was not destroyed by heat and trypsin treatment but by neuraminidase or periodic acid treatment suggesting that mC2bR reacted by its carbohydrate moiety with C2b, and immune adherence could be inhibited by D-glucose and D-galactose but not by their optical antipodes, L-glUCose and L-Galactose.

TL;DR: The XI-23 antibody reacted with all of the 89 cell suspensions carrying the HLA-A3 antigen (100% cytotoxicity) among a total of 191 suspensions tested, and can be considered as a good Hla-typing reagent.

TL;DR: Studies with Clq deprived of its globular parts by peptic digestion indicate that the collagen-like regions of Clq are involved in fibronectin binding, and collagenase treatment of Cl q abrogates its fibronECTin binding capacity.

TL;DR: Extracellular products have been purified from group A Streptococcus pyogenes culture supernatant fluids and their mitogenicities have been tested on rabbit and mouse lymphocytes and the gamma-fraction was very weakly mitogenic and had the capacity to reduce the stimulation induced by a T-cell mitogen, but not by a B- cell mitogen such as Nocardia.