International Journal of Bioassays 

About: International Journal of Bioassays is an academic journal. The journal publishes majorly in the area(s): Population & Phytochemical. It has an ISSN identifier of 2278-778X. Over the lifetime, 894 publications have been published receiving 2535 citations.

Evaluation of coronavirus in tears and conjunctival secretions of patients with SARS-CoV-2 infection

Abstract: Objective: This study aimed to assess the presence of novel coronavirus in tears and conjunctival secretions of SARS-CoV-2 infected patients. Methods: A prospective interventional case series study was performed, and 30confirmed novel coronavirus pneumonia (NCP) patients were selected at the First Affiliated Hospital of Zhejiang University from January 26, 2020, to February 9, 2020. At an interval of 2–3 days, tear and conjunctival secretions were collected twice with disposable sampling swabs for reverse transcription-polymerase chain reaction (RT-PCR)assay. Results: 21 common type and 9 severe type NCP patients were enrolled. Two samples of tear and conjunctival secretions were obtained from the only one patient with conjunctivitis yielded positive RT-PCR results. 58 samples from other patents were all negative. Conclusion: We speculate that SARS-CoV-2 may be detected in the tears and conjunctival secretions in NCP patients with conjunctivitis.

Quality Improvement of the DNA extracted by boiling method in Gram negative bacteria

Abstract: Several methods of Deoxyribonucleic acid (DNA) extraction have been applied to extract bacterial DNA. The amount and the quality of the DNA obtained for each one of those methods are variable. The study aimed to evaluate bacterial DNA extraction using conventional boiling method followed by alcohol precipitation . DNA extraction from Gram negative bacilli was extracted and precipitated using boiling method with further precipitation by ethanol. The extraction procedure performed using the boiling method resulted in high DNA yields for both E. coli and K. pneumoniae bacteria in (199.7 and 285.7μg/ml, respectively) which was close to control method (229.3 and 440.3μg/ml). It was concluded that after alcohol precipitation boiling procedure was easy, cost-effective, and applicable for high-yield quality of DNA in Gram-negative bacteria.

Impact of boron deficiency on plant growth

Abstract: Boron (B) is a unique non-metal essential microelement for plants and constantly needed throughout plants life for normal growth and development. Boron deficiency became a world-wide problem in agricultural areas as its management is challenged due to the very minor difference in its deficiency and toxicity. The effects of insufficient B supply on various physical and growth parameters of plant can lead to plant death. The major roles of boron in plant development, the conditions which lead to deficiency, B deficiency symptoms and methods to overcome B deficiency are summarized in the present review work.

Methodological advancements in green nanotechnology and their applications in biological synthesis of herbal nanoparticles

Abstract: The recent technological advancement in nanotechnology has opened new avenues for research and development in the field of herbal and medicinal plants biology. Development of reliable and eco-friendly processes for synthesis of nanoparticles is an important step for introduction of applications of nanotechnology into herbal research. In the past decade, there has been much concern about the methodological advancement of technology for synthesis and characterization of herbal and medicinal plants mediated nanoparticles. The present article provides a comprehensive review on the recent advances brought into methodology for biological and eco-friendly synthesis and characterization of herbal and medicinal plants mediated nanoparticles.

Phytochemical and bioactivity evaluation of Senna didymobotrya fresen irwin used by the Nandi community in Kenya

Abstract: Medicinal plants play a vital role in the traditional treatment of various diseases in Africa and the world at large. In Nandi county rift valley province in Kenya Senna didymobotyya is one of the most valued plants traditionally, this is mainly attributed to the famous mursik milk in which the plant is used as a preservative. A study was carried out to investigate the bioactivity of the plant leaves. Organism tested were Escherichia coli, Salmonella typhi, Enterobacter aerogenes , Klebsiella sp., Streptococcus pyogenes isolated from clinical specimens, and Proteus vulgaris and Bacillus cereus , obtained as standard isolates from commercial source. From the study Senna didymobotrya was found to contain secondary metabolites, saponins, flavonoids, tannins, phenols, steroids, steroidal ring (glycone portions of the glycoside), steroidal nucleus (glycone portion of the glycoside) and cardiac glycosides. The plant leaves were found not to contain alkaloids, terpenoids and anthroquinones. The bioactivity of the plant extract observed in this study can be attributed to the presence of these phytochemicals. Results from the bioassay Senna extract showed the highest zone of inhibition was obtained against Bacillus cereus at 500 mg/ml and its minimum inhibitory concentration was 31.25 mg/ml. The extract was also active against all the organisms at 500 mg/ml. Its minimum inhibitory concentration was 250 mg/ml for Streptococcus pyogenes , Escherichia coli and Proteus vulgaris .