Showing papers in "Journal of Biomimetics, Biomaterials and Biomedical Engineering in 2020"
TL;DR: The ROIs obtained using the proposed classifier-based segmentation algorithm was compared with the ground truth annotated by the radiologists and the performance of the proposed algorithm was evaluated.
Abstract: Breast cancer occurs as a result of erratic growth and proliferation cells that originate in the breast. In this paper, the classifiers were used to identify the abnormalities on mammograms to get the region of interest (ROI). Before classifier based segmentation, noise, pectoral muscles, and tags were removed for a successful segmentation process. Then the proposed approach extracted the brightest regions using modified k-means. From the extracted brightest regions, shape and texture features were extracted and given to classifiers (KNN and SVM) and marked as ROI only those non-overlapping abnormal regions. The ROIs obtained using the proposed classifier-based segmentation algorithm was compared with the ground truth annotated by the radiologists. The datasets used to evaluate the performance of the proposed algorithm was public (MIAS) and local datasets (BGH and DADC).
16 citations
TL;DR: An AI based computer-aided diagnosis tool, which can classify abnormalities by reading chest X-ray so that it could assist the doctors in arriving at quick diagnosis is developed.
Abstract: Though India being home of one out of every six people in the globe, is facing an arduous task of providing healthcare service, especially to the large number of patients in remote areas due to lack of diagnosis support systems and doctors. It is reported that hospitals in rural areas have an insufficient radiologist due to which thousands of cases are usually handled by single doctor. In this context, we aim to develop an AI based computer-aided diagnosis tool, which can classify abnormalities by reading chest X-ray so that it could assist the doctors in arriving at quick diagnosis. We have employed a Convolutional Neural Network (CNN) designed by Google known as XceptionNet to detect those pathologies in ChestX-ray14 data. Further, same data is being used for executing other CNN- ResNet. Finally, both the results obtained are compared to assess the superior CNN model for X-ray level diagnosis.
16 citations
TL;DR: In this paper, the authors reported a method to extract hydroxyapatite from bovine bone without producing hazardous residue, which has been proven to yield a high amount of pure hydroxypatite as well as reducing the use of hazardous reagent.
Abstract: Bovine bone is a considerable source for the production of hydroxyapatite. The recent study reported a novel method to extract hydroxyapatite from bovine bone without producing hazardous residue. The bovine bones were cut and boiled in the opened chamber followed by boiling in pressurized tank. The bones were then soaked into 95% ethanol. Calcination was then conducted in 800°C, 900°C and 1,000°C, for 2 hours. The result was then grinded and sieved. The powder then was characterized using Fourier transform infrared (FT-IR), Scanning electron microscopy (SEM) and X-ray diffraction analysis (XRD) to measure the purity of hydroxyapatite. It is concluded that the hydroxyapatite derived from this process showed 100% purity, resulting 35.34 ± 0.39% w/w from the wet bone weight and 72.3% w/w from the dried weight. The present extraction method has been proven to yield high amount of pure hydroxyapatite as well as reducing the use of hazardous reagent.
12 citations
TL;DR: Clinical-experimental justification of differentiated application of osteosynthesis methodics and application of cannulated screw with bioactive coverage on the basis of natural hydroxiapatite for patients of old and senile age with the medial fracture of femur’s neck (MFFN) is justified.
Abstract: The goal of our research is clinical-experimental justification of differentiated application of osteosynthesis methodicsand application of cannulated screw with bioactive coverage on the basis of natural hydroxiapatitefor patients of old and senile age with the medial fracture of femur’s neck (MFFN) with taking into account the stability grade of fracture by Pauwels classification and motional activity of patients before trauma. The present research is based upon clinical observation of 110 old and senile patients with supcapital and transcervical femur’s neck fractures. With the purpose of scientific justification and detailed research of different types of MFFN osteosynthses experimental stand tests were conducted with the use of cannulated screws and methodic of vertical ostesynthesis by H. Kuntscher for determining and comparison of density and strength of experimental systems “fixator-bone” in static regime over MFFN models. Cannulated screws with bioactive coverage on the basis of natural hydroxyapatite were applied for 16 patients among all operated by us. Time of rehabilitation of supporting and motional functions of the limb has been decreased by using these implants among patients with grades I and II (by Pauwels classification). Thanks to osteoinductive and osteoconductive features of bioactive coverage on the basis of natural hydroxyapatite, rehabilitation of operated limb’s function was conducted in shorter terms and faster rate. For the purpose of obtaining the dynamic stability of osteosynthesis and conduction of accelerated rehabilitation cannulated cervical screw was developed and applied in practice. This screw is different in the design of spiral part and the presence on its surface of bioactive coverage on the basis of natural hydroxyapatite.
8 citations
TL;DR: In this article, the reaction of bone tissue to installed titanium implants, which are bioinert in comparison with other metals used in traumatological and orthopedic practice, but which have a negative effect on bone tissue in the implant-to-bone area, was studied.
Abstract: This work is devoted to studying the reaction of bone tissue to installed titanium implants, which are bioinert in comparison with other metals used in traumatological and orthopedic practice, but which have a negative effect on bone tissue in the implant-to-bone area. In order to increase the affinity of the implant surface with bone tissue, it was proposed to use synthetic bioactive calcium phosphate mineral coating by applying it on titanium implants, which ultimately will make it possible to maintain the quality of the bone tissue around the implant after osteosynthesis or prosthetics. During the comparative research an in vivo experiment was conducted in which quality change of peri-implant zone of bone tissue when using titanium implants with synthetic bioactive calcium phosphate mineral coating and titanium implants without bioactive coating was analyzed. To analyze the results of the study, we used the X-ray computed tomography method, for a detailed assessment of the X-ray density and bone mineral density of the peri-implant zone. According to the results of the experiment, it was concluded that the reaction of bone tissue to synthetic bioactive calcium phosphate mineral coating of titanium implants was reduced, what ensures the preservation of bone tissue quality in the postoperative period at a high level.
6 citations
TL;DR: In this paper, the structural behavior of the synthesized ceramics was examined by means of X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM).
Abstract: The ceramics in the system CaO–MgO–SiO2 has recently attracted a great deal of attention because they display a good in vitro bioactivity and have potential use as bone implants. Biphasic calcium-magnesium-silicate ceramics were prepared by a sol-gel method. The dried gel with chemical composition 3CaO.MgO.2SiO2 was thermally treated at 1200 °C for 2 hrs. The structural behavior of the synthesized ceramics was examined by means of X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). Merwinite crystalline phase and akermanite phase were recognized. Then, porous akermanite/merwinite scaffolds were prepared to utilize polymer sponge method and evaluated by employing SEM. Furthermore, bone marrow stromal cells (BMSC) adhesion and proliferation on the scaffolds were evaluated by MTT assay test. Differentiation of the cells was assessed by measuring alkaline phosphatase (ALP) activity. The results demonstrated that BMSC adhered and spread well on akermanite scaffolds and proliferated with the increase in the culture time, and the differentiation rate of osteoblasts on scaffolds was comparable to that on blank culture plate control. Thus, the obtained results presented that the akermanite/merwinite scaffolds deserve attention for bone tissue engineering applications.
6 citations
TL;DR: In this paper, a sensor system was designed and manufactured to measure the interface pressure between the limbs and the orthosis or between the stump and the socket prosthesis, which is also used to determine the value and location of corrective forces when using orthosis to correct deformities in the limbs or spine.
Abstract: In this study, a sensor system was designed and manufactured to measure the interface pressure between the limbs and the orthosis or between the stump and the socket prosthesis. It is also used to determine the value and location of corrective forces when using orthosis to correct deformities in the limbs or spine. The sensor system consists of a force sensor mat (43.69 x 43.69 mm), Arduino, LCD monitor, and connecting cables. When comparing the device manufactured in this study with other devices, this device is characterized as lightweight, easy to move, low cost and gives instant pressure on the LCD screen. In this study, the interventional pressure measurement system was manufactured and programmed and compared to the F-Socket measurement system. The practical part was tested on a patient suffering from med-lateral instability in the ankle joint at the right leg. The interventional pressure between the leg and orthosis was measured in two ways at the regions (posterior, lateral, medial) .the first method using the pressure measurement system manufactured in this study and the second method by using the F-Socket device. The test results showed that the values of pressure readings using the manufactured system are close to the values of pressure readings obtained when using the F-Socket device. This indicates that the accuracy of the readings of the manufactured system and can be used for research purposes as well as in the process of placing pads in the appropriate place within the orthosis to correct the deformity or help to provide valuable information on the manufacturing, modification and fitting of prosthetic sockets. despite its simple structure and low cost as compared with F-Socket devise.
5 citations
TL;DR: Polyurethane-SWNT scaffolds contained metronidazole benzoate presented appropriate support of DPSCs adhesion and proliferation and biomimetic architecture like the structure of dental ECM.
Abstract: Tooth loss due to periodontal disease, dental caries, trauma or a variety of genetic disorders causes an adverse inability in adult’s lives. It is proved that biodegradable composite scaffolds in dental tissue engineering could play crucial role. To inhibit bacterial colonization in dental structure noticeable research concerning the drug delivery approach has been administrated. Nanostructures retain and release drug molecules more efficiently and continuously than other microstructure. In the present research, composite electrospun nanofibers of polyurethane-Single-walled carbon nanotube (SWNT) by the different mass ratios of metronidazole benzoate were prepared. Physico-chemical characterization of scaffolds including Scanning electron microscopy (SEM), uniaxial tensile testing and Ultraviolet-Visible (UV-Vis) spectroscopy analysis was operated. Culture of dental pulp stem cells (DPSCs) to evaluate cells behavior was carried out. The role of nanofiber diameters and drug content on releasing profile of the scaffolds was investigated. The median diameter of the nanofibrous scaffold was reduced from 330 ± 4 to 120 ± 4 nm. Ultimate stress and Young modulus of the scaffolds by enhancement of drug content increased from 0.28 ± 0.05 up to the 1.8 ± 0.05 MPa and 0.87 ± 0.05 up to the 4.4 ± 0.05 Mpa respectively. According to the result, prolonged and continuous releasing profile of the drug molecules was achieved. As the content of the drug increased, the drug was released continuously. It means that two parameters of fiber's diameter and drug ratio affected the releasing behavior of composite structures. Polyurethane-SWNT scaffolds contained metronidazole benzoate presented appropriate support of DPSCs adhesion and proliferation and biomimetic architecture like the structure of dental ECM.
5 citations
5 citations
TL;DR: Based on the results, it can conclude that biocomposite bacterial cellulose-chitosan-glycerol showed suitable characteristics as a dura mater artificial.
Abstract: Bacterial cellulose and chitosan have been widely developed for biomaterial applications, one of which is used as a dura mater artificial. In designing dura mater artificial, there are several criteria that must be met, one of which is mechanical that can be seen through tensile strength and elongation value. In previous study, the mechanical properties of biocomposite bacterial cellulose-chitosan still too rigid and did not meet the standard. This research was conducted to determine the effect of the addition of glycerol concentration to the physical and biological of bacterial cellulose-chitosan membrane. Bacterial cellulose membranes with the addition of glycerol concentration of 0%; 0,25%; 0,5% and 0,75% were dried with oven and immersed for 6 hours in 0.5% chitosan solution. Characterization was performed by functional group, morphology, tensile strength, swelling, degradation and cytotoxicity test. Based on the results, it can conclude that biocomposite bacterial cellulose-chitosan-glycerol showed suitable characteristics as a dura mater artificial.
5 citations
TL;DR: This paper proposes an advanced thresholding technique for the segmentation of brain MRIs based on the biologically inspired Ant Colony Optimization (ACO) algorithm, where the texture features are assumed as heuristic data.
Abstract: MR Brain Image Segmentation is an important step in brain image analysis. It facilitates the automatic interpretation or diagnosis that helps in surgical planning, estimating the changes in the brain’s volume for various types of tissues, and recognizing different neural disorders. Many neurological disorders like epilepsy, Alzheimer’s, tumor, and cancer can be effectively quantified and analyzed by finding the volume of the brain tissues such as White Matter (WM), Gray Matter (GM), and Cerebro Spinal Fluids (CSF). In manual segmentation of brain MRIs physicians manually determines the boundaries of different objects of interest and it is time-consuming and difficult. Thus, several accurate automatic brain MRI segmentation techniques with different levels of complexity have been proposed. This paper proposes an advanced thresholding technique for the segmentation of brain MRIs based on the biologically inspired Ant Colony Optimization (ACO) algorithm. Here the texture features are assumed as heuristic data. The experimental results for the T1-weighted brain MRIs have shown high accuracy than the conventional such as Fuzzy C-Means (FCM), Expectation-Maximization (EM), Improved Bacterial Foraging Algorithm (IBFA), and Improved Particle Swarm Optimization (IPSO).
TL;DR: In this article, phase composition, morphological and bioactivity properties of silicon-and carbonate-doped biomimetic hydroxyapatite synthesized by precipitation from aqueous solutions in the presence of different amounts of citrate ions were investigated.
Abstract: The present study investigated the phase composition, the structural, morphological, and bioactivity properties of silicon- and carbonate-doped biomimetic hydroxyapatite synthesized by precipitation from aqueous solutions in the presence of different amounts of citrate ions. The X-ray diffraction and Fourier transform infrared spectroscopy analyses confirmed that all the samples exhibited single-phase. Base on the results of the morphological study, all the obtained samples consisted of porous agglomerated particles made up of tiny crystallites in the nanometer range. The change in structural order, as well as the decrease in particle size and degree of crystallinity result from the presence of citrate ions were revealed by X-ray diffraction, dynamic light scattering, and scanning electron microscopy analyses. Bioactivity properties of samples were studied by analyzing their bioresorbability in physiological saline (ω (NaCl) = 0.9%) and evaluating their solubility in SBF solution after a certain period of soaking time. The amount of the released Ca2+ ions was found to increase with the increasing concentration of citrate ions introduced in the synthesis process. The better solubility of material with the presence of citrate ions was beneficial in the growth of apatite on its surface that made produced material more biocompatible.
TL;DR: Application of engineered PTX-loaded HAP scaffold in bone regeneration can act on behalf of organic matrix production, but not angiogenesis and mineralization.
Abstract: Background: Hydroxyapatite (HAP), as a common biomaterial in bone tissue engineering, can be fabricated in combination with other osteogenic agents. Pentoxifylline (PTX) is demonstrated to have positive roles in bone defect healing. Since local administration can diminish the systemic side effects of the drug, the objectives of the current in vitro study were to find the effects of PTX on the osteoblast functions for tissue engineering applications. Methods: a HAP scaffold was fabricated by casting the HAP slurry within polyurethane foam. The scaffold was enriched with 5 mg/mL PTX. Alginate (Alg) was used as drug carrier to regulate the PTX releasing rate. MG-63 osteosarcoma cells were cultured on 3D scaffolds and 2D Alg films in the presence or absence of PTX. Results: PTX did not affect the cell viability, attachment and phenotype. Also, the ultrastructure of the scaffolds was not modified by PTX enrichment. Alizarin red S staining showed that PTX has no effect on calcium deposition. Besides, Raman confocal microscopy demonstrated an increase in the organic matrix formation including proline, valine and phenylalanine deposition (represented collagen). Although PTX increased the total protein secretion, it led to a decrease in the alkaline phosphatase activity and vascular endothelial growth factor (VEGF) content. PTX reduced the hydration and degradation rates and it was released mainly at the first 24 hours of incubation. Conclusion: Based on our in vitro study, application of engineered PTX-loaded HAP scaffold in bone regeneration can act on behalf of organic matrix production, but not angiogenesis and mineralization.
TL;DR: In this paper, the authors evaluated the effect of immersion of carbonate apatite type B using the dissolution-precipitation method on the formation of porous C-Ap type B with calcium sulfate precursor hemihydrate.
Abstract: Introduction: Carbonate apatite type B (C-Ap) has been used as a bone replacement material because of its osteoconductive properties. Clinically, the pores formed in bone replacement material aid in cell mobility and nutrient supply, thereby increasing the bone regeneration ability. CO32- ions found in this material are useful for maintaining a stable physiological environment in the bone in order for it to be easily absorbed by osteoclasts. Porous C-Ap type B is formed using the dissolution–precipitation method by immersing porous anhydrous CaSO4 in a mixture of carbonate and phosphate solutions. Purpose: The present study aimed to evaluate the effect of immersion ofCaSO4using the dissolution–precipitation method on the formation of porous C-Ap type B with calcium sulfate precursor hemihydrate. Method: Porous C-Ap type B was produced usinga mixture of calcium sulfate hemihydrate precursors with 50 wt% polymethylmethacrylate (PMMA) porogen and distilled water. After hardening, the calcium sulfate dihydrate containing PMMA was burned in an oven at 700°C for 4 h to remove the PMMA. The specimen was immersed in a mixture of sodium phosphate (Na3PO4) and sodium carbonate (Na2CO3) for 6, 12, and 24 h. Phase testing through X-ray diffraction (XRD) using CuKα radiation at 40 kV and 40 mA was performed. Attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR, Thermo Fisher Scientific, Waltham, Massachusetts, USA) was used for detecting the functional groups of CO32- and PO42-. Results: XRD results showed the formation of C-Ap at 6 and 12 h, but the anhydrous CaSO4 phase remained; alternatively, this phase was absent after 24 h of immersion phase andFTIR showed the presence of the functional groups of CO32- compounds. Conclusion: Porous C-Ap type B can be formed from CaSO4 precursors after 24 h of immersion using the dissolution–precipitation method.
TL;DR: The higher p53 and/or hTERT immunoexpression, the higher or worse the level of histopathological grading of oral cavity squamous cell carcinoma (poorly differentiated).
Abstract: Oral cancer account for 30% of all malignant tumors in the head and neck, more than 90% of these cancers are squamous cell carcinoma. The p53 tumor suppressor gene known as \"the guardian of the genome\" has a major function in cell cycle control and act as a main defense against cancer, the occurrence of genomic instability causes inactivation and mutation of p53, which related to the progression of cancer cells and poor prognosis for patients. Human telomerase reverse transcriptase (hTERT), a catalytic protein subunit of the complex telomerase enzyme, prevents telomere erosion during DNA replication, thus allowing cells to escape the aging cell step. The relationship between hTERT and malignant transformation is around 90%, the detection of hTERT is associated with malignancy that leads to a worse prognosis which increases immortality or continuous growth in cancer cells. This study was conducted with retrospective cross sectional using immunohistochemical p53 and hTERT smear in 30 paraffin blocks of squamous cell carcinoma of the oral cavity, held at the Anatomical Pathology Department of Hasan Sadikin Hospital Bandung. P53 and hTERT immunoexpression were correlated with histopathological grading of squamous cell carcinoma of oral cavity (SCC) and statistically analyzed with Rank Spearman correlation with significance of p<0.05 (95%) and Kendall Coefficient of Concordance with significance of p <0.05% (95%). The results showed a significant positive correlation between p53 immunoexpression with histopathological grading (rs=0.497, p-value = 0.005), between hTERT immunoexpression and histopathological grading (rs=0.441, p-value=0.015), and between p53 and immunoexpression hTERT with histopathological grading (W=0.568, p-value=3.99E-08) Conclusion: the higher p53 and/or hTERT immunoexpression, the higher or worse the level of histopathological grading of oral cavity squamous cell carcinoma (poorly differentiated).
TL;DR: Black cumin extract can make wound healing in tooth extraction sockets in patients faster in the treatment group when compared to the control group, and has the best effect on wound healing.
Abstract: Objective: This study aims to investigate the effects of black cumin extract (Nigella sativa) on the process of wound healing in tooth extraction sockets. Material and Methods: This study involved 24 patients from the Department of Oral and Maxillofacial Surgery grouped into the control and treatment groups. After extracting the first or second molar left or right in adult patients, the black cumin extractwas applied to the socket in the treatment group as soon as possible while it was not applied in the control group. The sockets were divided into 1,2,3 groups, which were treated as black cumin extracts of 10%, 12%, and 14% respectively. On day 1,3,7, patients were recalled and sockets size were calculated to analyze wound healing. Results: Black cumin was found to be able to make the wound healing faster in the treatment group when compared to the control group. Although the effect was not immediately apparent on the second day, on the seventh day the group treated with black cumin extract had a smaller sizing average compared to the control group. The concentration of 12% black cumin extract was found to have the best effect on wound healing. Significance of findings: Black cumin extract can make wound healing in tooth extraction sockets in patients.
TL;DR: The aim of the study was to identify the effect of cheese and milk consumption on salivary buffer capacity of students aged 10-12 year at Sekolah Dasar Negeri 57 Banda Aceh and showed significant differences (p<0.05) salivARY buffer capacity after consumption of milk compared with cheese.
Abstract: Saliva have significant roles in maintaining the health of oral cavity. Salivary flow and composition of saliva play a critical role. Saliva also serves as buffer system. Increasing the salivary flow will increase salivary buffer capacity and pH of saliva. Salivary buffer capacity serves to protect the teeth from the acid that comes from food which is formed by acidogenic microorganisms. Milk and dairy are good diet for children that recommended by American Heart Association (AHA). Cheese as one of dairy mild can increase the salivary flow, that help keep the dental hygiene, and prevent bacteria sticking to the teeth. The aim of the study was to identify the effect of cheese and milk consumption on salivary buffer capacity of students aged 10-12 year at Sekolah Dasar Negeri 57 Banda Aceh. Salivary buffer capacity after consume cheese measured after chewing cheese 4 gram about 32 times whereas salivary buffer capacity after consume milk consumption was measured after gargling 25 ml milk 34 times. Salivary buffer capacity measured with Saliva-check buffer (GC). Results of Wilcoxon test showed significant differences (p<0.05) salivary buffer capacity after consumption of milk compared with cheese. Capacity buffer salivary after consumption of cheese higher than milk. In conclusion, cheese and milk consumption have effect on salivary buffer capacity. Salivary buffer capacity after cheese consumption is higher than after milk consumption.
TL;DR: This study presents statistics on osteoporosis and lower energy trauma among patients in the Russian Federation and abroad and analysis of publications representing various methods of fracture treatment helps to use the most optimal methods of relevant fractures treatment.
Abstract: This study focuses on problems of treatment of proximal humeral fractures occurring against the background of osteoporosis. It presents statistics on osteoporosis and lower energy trauma among patients in the Russian Federation and abroad. The high susceptibility to osteoporotic changes in the bone structure of the male population of Russia compared to the global statistics of osteoporosis among men was emphasized. The main classifications used in the clinical practice of orthopedists and traumatologists allow to identify the type of fracture by clinical and anatomical basis and to structure the existing patient management tactics to achieve the most optimal result of treatment and restoration of limb function. There is an analysis of publications representing various methods of fracture treatment, which helps to use the most optimal methods of relevant fractures treatment.
TL;DR: Finite element (FE) simulations using COMSOL Multiphysics on a CAD model of a knee are effectuated to show the effect of several loads and strains on the knee and indicates that the orthotropic model depicts a more realistic stress distribution of the knee as it reveals the detailed anatomy of the entire knee structure.
Abstract: The knee at times undergoes a surgical process to substitute the weight-bearing surfaces of the knee joint. This procedure relieves the pain and disability around the knee joint. This research paper studied the knee arthroplasty, also referred to as knee replacement. This work was aided with computer vision for visual and accuracy. Autodesk fusion 360 and the stl files were used to generate cemented, posterior stabilised knee prosthesis and imported into the COMSOL Multiphysics software. Then, the three-dimensional models of the total knee arthroplasty (TKA) prosthetic structure are produced. The prosthetic components are modelled as linear isotropic elastic materials. Finite element (FE) simulations using COMSOL Multiphysics on a CAD model of a knee are effectuated to show the effect of several loads and strains on the knee. FE analysis of the model indicates that the orthotropic model depicts a more realistic stress distribution of the knee as it reveals the detailed anatomy of the entire knee structure. The computational results of this work displayed a fair agreement with experimental information from the literature.
TL;DR: The efficacy of pathogenetic correction of metabolism in 211 patients with diaphyseal hip and shin bones fractures had been analyzed and main group provide better physical component of the life quality and blood parameters functional recovery.
Abstract: The efficacy of pathogenetic correction of metabolism in 211 patients with diaphyseal hip and shin bones fractures had been analyzed. Patients were divided into the main and control groups. Intravenous infusions of 1.5% sodium meglumine succinate solution were administered, and infusion therapy with isotonic solution to the patients of the comparison group. The parameters of functional activity and peripheral blood dates had been analyzed. In the perioperative period main group provide better physical component of the life quality and blood parameters functional recovery.
TL;DR: Runners can adjust different joints angles to maintain a similar GRF during long-distance running on different sports surfaces, showing no significant differences in GRF and average loading rate.
Abstract: Running is one of the most accessible physical activities and long-distance running has attracted extensive attention in the past several years. While the incidence of running injuries, especially to the lower extremities, has increased. The objective of this study was to investigate the differences in ground reaction forces (GRF) and kinematic param between long-distance runners before and after long-distance running on treadmill (TM), asphalt road (AR), and plastic track (PT). Eight-camera Vicon motion analysis system was used to measure the hip, knee and ankle motion param of 10 healthy male subjects at a speed of 2.8 ± 0.2m/s. The hip, knee and ankle kinematics and the relationship of joint angles of lower limbs in the sagittal plane, coronal plane and transversal plane were analyzed. Ground reaction force (GRF) was collected using an AMTI force platform. The results showed that there were no significant differences in GRF and average loading rate (VALR). There was no significant difference in the range of motion (ROM) of ankle and hip after long-distance running on three surfaces compared to pre-test. Compared with stance-period of pre-test, the stance-period of AR and PT were significantly longer. Post hoc analyses exhibited the stance-period of AR and PT were longer than TM. In conclusion, runners can adjust different joints angles to maintain a similar GRF during long-distance running on different sports surfaces.
TL;DR: This paper used Enhanced Independent component analysis to perform the segmentation of gray matter using modified K means, Expected Maximization and Hidden Markov random field to provide better spatial correlation that overcomes in-homogeneity, noise and low contrast.
Abstract: Medical segmentation is the primary task performed to diagnosis the abnormalities in the human body. The brain is the complex organ and anatomical segmentation of brain tissues is a challenging task. In this paper, we used Enhanced Independent component analysis to perform the segmentation of gray matter. We used modified K means, Expected Maximization and Hidden Markov random field to provide better spatial correlation that overcomes in-homogeneity, noise and low contrast. Our objective is achieved in two steps (i) initially unwanted tissues are clipped from the MRI image using skull stripped Algorithm (ii) Enhanced Independent Component analysis is used to perform the segmentation of gray matter. We apply the proposed method on both T1w and T2w MRI to perform segmentation of gray matter at different noisy environments. We evaluate the the performance of our proposed system with Jaccard Index, Dice Coefficient and Accuracy. We further compared the proposed system performance with the existing frameworks. Our proposed method gives better segmentation of gray matter useful for diagnosis neurodegenerative disorders.
TL;DR: One visit pulpectomy followed by restoration with porcelain crown and fiber posts can be undertaken to achieve the optimal functions of teeth, namely aesthetic, mastication, phonetics, and protection of supporting tissues function.
Abstract: Introduction. Trauma injury in the oral cavity due to collisions can cause tooth fracture. Ellis class III fracture is a fracture of the crown with an open pulp. Teeth with exposed pulp will cause irritation of the pulp resulting in pulp inflammation (pulpitis). One visit pulpectomy and jacket crown with posts were carried out to achieve optimal dental functions. Case report. A 27-year-old male patient came to Dental Hospital of Universitas Gadjah Mada complained about his broken front teeth due to accident three days earlier. The crown of the maxillary right lateral incisor and the maxillary left central incisor with open pulp was seen. The tooth 11 was avulsion, which lost at the location of accident. The diagnosis of 12 and 21 were Ellis class III fracture. Initially an infiltration anaesthesia was done, followed by one visit root canal treatment (RCT). On the second visit, the teeth were asymptomatic, hence the jacket crown preparation and fiber post insertion were performed. On the third visit, a porcelain jacket crown was cemented into teeth 12 and 21. A week later the patient came for recall and no complaints were noted. Conclusion. One visit pulpectomy followed by restoration with porcelain crown and fiber posts can be undertaken to achieve the optimal functions of teeth, namely aesthetic, mastication, phonetics, and protection of supporting tissues function.
TL;DR: The result showed that a method of correction forces applied by using the air chamber to correct deformities was successful when applied to a patient with varus foot deformity, reducing the cost and time to treat deformity and improvement of the gait cycle, the value of ground reaction force on the body and footprint of the patient.
Abstract: It is known that the deformities that affect the lower limbs are multiple in terms of location and severity of the injury. These deformities are treated with the use of orthosis, which varies depending on the type of deformity and the area of injury. The principle on which the orthosis depends on the treatment of deformities is applying three-point pressures. These pressures are applied during the manufacturing process of the orthosis but with the time when the deformation response to treatment by corrected forces these mean the value and location of these corrective forces will change and this requires the manufacturing of a new orthosis. This requires costs in money and time to re-correct these forces. In this study, a force correction system was suggested that could change the value and location of corrective forces without need to manufacture a new orthosis and within a few minutes by placing an air chamber on the side of the orthosis. When increasing or withdrawing the amount of air inside the air chamber, the magnitudes of corrected force change according to the required value, this process providing comfort to the patient and reducing the effort of the professional staff supervisor (orthotist) to correct the deformity. The result showed that a method of correction forces applied by using the air chamber to correct deformities was successful when applied to a patient with varus foot deformity where the pressure generated from the air chamber on the leg was measured by using the F-Socket devise at the lateral side recorded a higher interface pressure (140Kpa) than the others sides due to pump the air to push the ankle joint for correction deformity without needing to manufacture of a new orthosis to change the values of correction forces thus reducing the cost and time to treat deformity, also the result showed improvement of the gait cycle, the value of ground reaction force on the body and footprint of the patient when comparing the results before and after the use of the orthosis by the patient.
TL;DR: The findings suggest PCL-CDM/fibrin can be considered as an appropriate hybrid scaffold for CTE applications and demonstrated that incorporation of CDM in PCL fibers results in improved cell viability.
Abstract: Electrospun fibers have demonstrated a remarkable potential as a framework structure in the fabrication of cartilage tissue engineering (CTE) scaffolds. Various extracellular matrices have been incorporated into electrospun scaffolds to mimic and simulate the extracellular environment. The objective of this study was to fabricate hybrid constructs using composite electrospun scaffolds based on poly (ε-caprolactone) (PCL) and cartilage-derived matrix (CDM) and fibrin hydrogel to improve the viability and differentiation of human adipose-derived stromal cells (ADSCs) for CTE applications.Initially, PCL and PCL-CDM electrospun mats were fabricated. Fibrin/ ADSCs hydrogel were seeded on PCL- CDM mats and arranged layer-by-layer using sandwich technique. This method has been employed to increase cell seeding and infiltration efficiency through the entire mass of the scaffold. Real-time reverse-transcription polymerase chain reaction (RT- PCR), were performed to examine the expression of collagen types II and X, SOX9 and aggrecan. The production of glycosaminoglycan (GAG) was also tested in vitro by Toluidine blue stain and biochemical assay in the cultured scaffolds.The findings demonstrated that incorporation of CDM in PCL fibers results in improved cell viability. Hematoxylin and eosin staining showed that the sandwich method resulted in homogenous cell seeding within the scaffold. Overall, the RT- PCR, biochemical and histological results, showed that incorporation of the CDM into PCL/fibrin sandwich scaffolds stimulated ADSCs chondrogenesis and produced the products which increased expression of chondrogenic genes. It also, enhanced GAG synthesis compared to PCL/fibrin scaffolds.These findings suggest PCL-CDM/fibrin can be considered as an appropriate hybrid scaffold for CTE applications.
TL;DR: The design solution presented may support the large user base of the SACH foot towards achieving enhanced gait characteristics during ambulation and demonstrates a novel design procedure for a prosthetic foot through an effective numerical implementation.
Abstract: The Solid Ankle Cushioned Heel (SACH) foot is a commonly prescribed prosthetic foot for the rehabilitation of lower limb amputees. From the viewpoint of its biomechanical performance, the foot is known to cause drop-off effect and asymmetry in amputee gait. Therefore, the objective of this work is to improvise the effective foot length ratio (EFLR) and the progression of the centre of pressure (CoP) of the SACH foot by providing a novel design approach that utilizes finite element analysis. Boundary conditions employed for evaluating the roll-over characteristics of prosthetic feet were numerically incorporated in this work. The non-linear mechanical behavior of the foot was included with the incorporation of large deformation, a hyperelastic material model and the Augmented Lagrangian contact formulation. Outcomes from the simulations were experimentally verified using an inverted pendulum-like apparatus, thereby substantiating the numerical approach. The design process of the SACH foot involved the modification of the elastic modulus of its components for enhancing the parameters of interest. Results obtained presented a 5.07% increase in the EFLR and a 9.29% increase in the anteroposterior progression of the CoP, which may improve amputee stability. The design solution presented may support the large user base of the SACH foot towards achieving enhanced gait characteristics during ambulation. Moreover, this work successfully demonstrates a novel design procedure for a prosthetic foot through an effective numerical implementation.
TL;DR: The biomaterial evaluated was shown to be non-cytotoxic, resorbable, and capable of inducing bone neoformation; however, more studies should be carried out to assess the application of this biomaterial in bone injuries.
Abstract: Biomaterials can be used in several areas of regenerative bioengineering, and is a viable option in the repair of bone injuries. A number of different types of biomaterials have been studied in relation to bone repair. Ceramics such as α-TCP have low fracture toughness compared to natural bone, so reinforcements such as wollastonite whiskers are developed so that they can be used in places with greater overload. This study aimed to evaluate the biocompatibility and bone neoformation of α-TCP plus 10% wollastonite whiskers, in vivo. To obtain the cement, α-TCP powders with or without 10% wollastonite whiskers were added to an aqueous solution containing 2.5% by weight of Na2HPO4 (anhydrous bibasic sodium phosphate). The biomaterial then became a paste, which was molded into the critical 5 mm defect made in the parietal bone of Wistar rats. Ten rats were divided into two groups. The animals from each group were euthanized within 30 days. Calvaries were removed and subjected to histological processing with Eosin and Hematoxylin. The implementation of the whisker biomaterial revealed the formation of intensely vascularized connective tissue in the implemented region; however, animals with the biomaterial α-TCP showed the formation of this tissue around the implemented region. On the other hand, intense bone resorption was observed only in the animals with Wollastonite Whiskers, but new bone formation in both groups. The biomaterial evaluated was shown to be non-cytotoxic, resorbable, and capable of inducing bone neoformation; however, more studies should be carried out to assess the application of this biomaterial in bone injuries.
TL;DR: This study evaluated fibroin films in some characteristics such as surface structure, chemical structure, tensile strength, absorbency, dehydration rate, biodegradation ability, pH determination, preventing bacteria ability and cytotoxicity test, and indicated that created FF is fulfilled with all the required properties of wound dressings.
Abstract: Background: Currently silk fibroin is used more and more in the biomedical researches, including a potential research direction in creating wound dressing. Turmeric powder is a natural drug with many properties suitable for treatment of burns such as anti-inflammatory, anti-bacterial, anti-fungal, especially reducing formation of scars. Methods: In this study, sericin is removed from the silk to obtain fibroin fiber. Fibroin fiber and turmeric powder are dissolved by formic acid adding calcium chloride (CaCl2). Created fibroin films (FF) are then evaluated in some characteristics such as surface structure, chemical structure, tensile strength, absorbency, dehydration rate, biodegradation ability, pH determination, preventing bacteria ability and cytotoxicity test. Results: All results indicated that created FF is fulfilled with all the required properties of wound dressings. Conclusions: This study is the first step to creating foundation and orientation for the development of commercial wound dressings.
TL;DR: Investigation of the effectiveness of different hardness of personalized custom insoles on plantar pressure redistribution in healthy young males during walking and running found CSI seems better than CHI because of redistributing the plantar Pressure by increasing the plantAR pressure of whole forefoot.
Abstract: The objective of this study was to investigate the effectiveness of different hardness of personalized custom insoles on plantar pressure redistribution in healthy young males during walking and running. Six males participated in the walking and running test (age: 24±1.6 years, weight: 67.9±3.6 kg, height: 175.5±4.7 cm). All subjects were instructed to walk and run along a 10m pathway wearing two different hardness insoles (i.e., hard custom insoles (CHI) and soft custom insole (CSI)) and control insole (CI) at their preferred speed. Peak pressure, mean pressure, maximum force, pressure-time integral were collected to analyze using SPSS. The plantar pressure of forefoot and medial midfoot were significantly increased and of lateral forefoot and lateral midfoot were decreased by both kinds of custom insoles in running tests. While the CHI significantly increased plantar pressure of the medial forefoot compared with the CSI and CI both in walking and running tests. The custom insoles showed significantly higher plantar pressure on medial midfoot. But CSI seems better than CHI because of redistributing the plantar pressure by increasing the plantar pressure of whole forefoot. Moreover, CSI showed significantly lower plantar pressure than CI and CHI at lateral midfoot during running test. The CHI causes significant high pressure at medial forefoot (MF), which may raise the risk of forefoot pain.
TL;DR: Analysis of the modern scientific data has shown that there is a great need to know the instrumental methods for studying human organs and systems, and their more accurate possibilities for identifying pathological processes, which will permit the use of the research method that in any particular case will be the most informative, which would provide timely diagnosis and its accuracy.
Abstract: The detailed study devoted to instrumental diagnostic methods of bone tissue research was performed by authors. The main idea which is the basis for this work is the analysis and study of X-ray methods for examining bone tissue, highlighting their advantages. As a result of the work, we analyzed published scientific data in Russian and foreign literary sources, which addresses many instrumental methods of bone tissue research. After experiments a detailed description of the most common research methods related to the study of bone tissue was given, its structure and other functions. Analysis of the modern scientific data has shown that there is a great need to know the instrumental methods for studying human organs and systems, and their more accurate possibilities for identifying pathological processes, which will permit the use of the research method that in any particular case will be the most informative, which would provide timely diagnosis and its accuracy.