Journal of Fluorescence 

About: Journal of Fluorescence is an academic journal published by Springer Science+Business Media. The journal publishes majorly in the area(s): Fluorescence & Chemistry. It has an ISSN identifier of 1053-0509. Over the lifetime, 4038 publications have been published receiving 65568 citations.

Editorial Metal-Enhanced Fluorescence

Abstract: As fluorescence spectroscopists we are mostly all yields, decreased lifetimes, increased photostability, and familiar with doing spectroscopy in bulk samples, where an increase in Forster transfer distances and directional the solutions are typically transparent to the emitted radia- emission Such opportunities are truly refreshing, because tion, and the fluorophores emit isotropically into free although fluorescence methodology and subsequent space and are observed in the far field There may be applications are routinely practiced in many laboratories changes in the refractive index, such as for a fluorophore around the world, little research on such basic principles in a membrane, but these changes typically have only has probably hindered fluorescence growth from further minor effects on the fluorophores free-space spectral revolutionalizing the analytical and clinical sciences For properties

Laurdan and Prodan as Polarity-Sensitive Fluorescent Membrane Probes

Abstract: The steady-state and dynamic fluorescence spectral properties of 2-dimethylamino-6-lauroylnaphthalene (LAURDAN) and several other naphthalene derivatives are summarized to illustrate their sensitivity to the polarity of the environment Results obtained both in solvents of different polarity and in phospholipid vesicles in two phase states are presented The emission red shift observed in polar solvents and in the phospholipid liquid–crystalline phase is explained on the basis of dipolar relaxation of solvent molecules surrounding the fluorescent naphthalene moiety of these probes In phospholipid environments, experimental evidence is shown that excludes the intramolecular relative reorientation of the dimethylamino and carbonyl groups in the naphthalene and the reorientation of the entire fluorescent moiety The solvent dipolar relaxation observed for LAURDAN and PRODAN in phospholipid bilayers has been attributed to a small number of water molecules present at the membrane interface A comparison between LAURDAN emission in phospholipid vesicles prepared in D2O and in H2O is also presented The definition and the derivation of the generalized polarization function are also discussed

Progress in lanthanides as luminescent probes.

Abstract: Lanthanides have recently found applications in different fields of biomolecular and medical research. Luminescent lanthanide chelates have created interest mainly due to their unique luminescent properties, such as their long Stokes’ shift and exceptional decay times allowing efficient temporal discrimination of background interferences in the assays, such as immunoassays. Recently, new organometallic complexes have been developed giving opportunities to novel applications, in heterogeneous and homogeneous immunoassays, DNA hybridization assays, high-throughput screening as well as in imaging. In addition, encapsulating the chelates into suitable matrix in beads enables the use of new members of lanthanides extending the emission wavelength to micrometer range and decays from a few microseconds to milliseconds. As the luminescence is derived from complicated intrachelate energy transfer, it also gives novel opportunities to exploit these levels in different types of energy transfer based applications. This review gives a short overview of recent development of lanthanide chelate-labels and discusses in more details of energy levels and their exploitation in new assay formats.

Fluorescence Study on the Interaction of Bovine Serum Albumin with P-Aminoazobenzene

Abstract: In this paper, the interaction between p-aminoazobenzene (PAAB) and BSA was investigated mainly by fluorescence quenching spectra, circular dichroism (CD) and three-dimensional fluorescence spectra under simulative physiological conditions. It was proved that the fluorescence quenching of BSA by PAAB was mainly a result of the formation of a PAAB-BSA complex. The modified Stern-Volmer quenching constant Ka and the corresponding thermodynamic parameters ΔH, ΔG and ΔS at different temperatures were calculated. The results indicated that van der Waals interactions and hydrogen bonds were the predominant intermolecular forces in stabilizing the complex. The distance r = 4.33 nm between the donor (BSA) and acceptor (PAAB) was obtained according to Forster’s non-radioactive energy transfer theory. The synchronous fluorescence, CD and three-dimensional fluorescence spectral results showed that the hydrophobicity of amino acid residues increased and the losing of α-helix content (from 63.57 to 51.83%) in the presence of PAAB. These revealed that the microenvironment and conformation of BSA were changed in the binding reaction.

Synthesis and Optical Properties of Anisotropic Metal Nanoparticles

Abstract: In this paper we overview our recent studies of anisotropic noble metal (e.g. gold and silver) nanoparticles, in which a combination of theory and experiment has been used to elucidate the extinction spectra of the particles, as well as information related to their surface enhanced Raman spectroscopy. We used wet-chemical methods to generate several structurally well-defined nanostructures other than solid spheres, including silver nanodisks and triangular nanoprisms, and gold nanoshells and multipods. When solid spheres are transformed into one of these shapes, the surface plasmon resonances in these particles are strongly affected, typically red-shifting and even splitting into distinctive dipole and quadrupole plasmon modes. In parallel, we have developed computational electrodynamics methods based on the discrete dipole approximation (DDA) method to determine the origins of these intriguing optical features. This has resulted in considerable insight concerning the variation of plasmon wavelength with nanoparticle size, shape and dielectric environment, as well as the use of these particles for optical sensing applications.