Showing papers in "Journal of Neuroscience Methods in 1984"

TL;DR: Developments of an open-field water-maze procedure in which rats learn to escape from opaque water onto a hidden platform are described, suggesting that they may lend themselves to a variety of behavioural investigations, including pharmacological work and studies of cerebral function.

TL;DR: The theory of operation of a discontinuous single-electrode voltage clamp using an ideal microElectrode (infinite response speed) and fixed (current-passing) duty cycle is extended by considering a microelectrodes which has a finite response speed, by allowing the duty cycle to be variable, and by considering the clamp noise.

TL;DR: It is found that predominantly grey matter regions of rat brain showed similar tritium concentrations when measured by either method, but that optical density reading of white matter regions indicated a lowertritium concentration than determined by direct measurement with scintillation counting.

TL;DR: A method is described which allows the Golgi-impregnation of neurons within a 'single' section or 'slice' of brain tissue 60-200 micron thick and allows the re-impRegnation of single sections.

TL;DR: The local application of cobalt reversibly blocks the calcium channel conductance and therefore synaptic transmission, which is a useful tool to discriminate between the origins of a response recorded with extracellular metal electrodes from central nervous system substructures.

TL;DR: Voltammetry at the modified electrodes gives evidence the catecholamines can be resolved from ascorbate, and that catechols, but not asCorbic acid, adsorb to the electrode surface.

TL;DR: A computer-based video image processing system is described that quantifies the binding of the neuroleptic drug, [3H]spiroperidol, to rat forebrain sections, using a calibration curve generated from 3H-containing tissue standards to produce autoradiographs of total binding or non-butaclamol-displaced ('non-specific') binding.

TL;DR: It is concluded that knowledge of mobile phase parameters, their mechanism, and manipulation is as important as the assay itself for practical utilization in selective amine separation.

TL;DR: The advantages are simplicity, and generality; the latter resulting from the empirical nature of the correction factor which can calibrate for multipleucleoli, split nucleoli, invisible fragments, nucleolar size changes, section thickness differences and any other factors that cause n to deviate from N.

TL;DR: A system is described for observing individual neurons and microelectrodes in 400 μm brain slices using infrared video microscopy using infraredVideo microscopy.

TL;DR: An index, W%, is proposed which is based on the product of the volumes of the damage on the right and left sides such that conditions of no injury or unilateral injury produce an index of zero.

TL;DR: A sensitive and specific procedure for quantifying plasma catecholamines using on-line trace enrichment high performance liquid chromatography with electrochemical detection (HPLC-EC) is described, which yields a lower working limit of detection of 25 pg norepinephrine and epinephrine per milliliter plasma.

TL;DR: D densitometric precision of a corrected vidicon scanner is not the limiting factor for quantitative autoradiographic densitometry, as well as acceptable between-session repeatability.

TL;DR: This work differs from previous procedures by identifying neurons first and then determining whether the nucleolus in each identified neuron is split or whole, and only a determination of the numbers of whole and split nucleoli for a sample population of neurons.

TL;DR: The expression of typical oligodendrocyte markers (2':3'-cyclic-nucleotide 3'-phosphodiesterase, galactocerebrosides and myelin basic protein) was greatly enhanced under these serum-free conditions as compared with cultures in serum-containing medium.

TL;DR: Deposits of WGA-HRP in the optic tract, optic radiations and corpus callosum were made to determine if this tracer is incorporated and transported by intact fibers and it was found that the effective uptake zone is approximately one half the size of the geniculate area containing reaction-product.

TL;DR: This report describes the use of a porous polymeric sleeve (Gore-tex) to direct nerve fiber growth after axotomy in 5 adult cats and revealed a complete absence of unintended reinnervation and a regeneration that was more substantial for motor than sensory axons.

TL;DR: The measurement error inherent in visual evaluation of respiratory disturbances during human sleep is investigated and the present paradigm may be useful in evaluating other types of measurement error.

TL;DR: An adaptation of a miniature microdrive allows unit recording and microiontophoresis in freely moving rats and suggests that this technique will be useful in studying drug actions on single neurons across a wide range of conscious behaviors.

TL;DR: An immunocytochemical procedure is described for reliably determining the hormone content of magnocellular neuroendocrine neurons that have been injected with Lucifer Yellow in slices of rat hypothalamus.

TL;DR: The construction of the device allows the interchange of vessels containing various perfusates during the perfusion, without having to interrupt the flow or alter the hydrostatic pressure, leading to good preservation of brain tissue.

TL;DR: An easily constructed, inexpensive and simple system is described for the superfusion of neural tissue that characterized the dynamics of endogenous dopamine and dihydroxyphenylacetic acid release from striatal tissue before and after exposure to potassium, amphetamine or cocaine.

TL;DR: In this article, a computerized image-processing system that employs a high resolution Datacopy Corporation charge-coupled device (CCD) camera is described, where the CCD camera generates 327,680 pixels (512 horizontal axis by 640 vertical axis), eight bits per pixel (256 grey levels).

TL;DR: The reduced silver procedure of Liesegang is modified and a gold treatment is added, and an excellent overall image of CNS structure is obtained.

TL;DR: A simple method for the separation of rat retinal sublayers was developed and viable cells of each of the sublayer could be cultured on a poly-L-lysine coated chamber for at least several weeks in Eagle's minimum essential medium supplemented with calf serum and glucose.

TL;DR: A simple method is described for the construction of electrode arrays consisting of insulated metal wires spaced at small, equal distances (0.1 mm) for field potential recording from in vitro brain slice preparations.

TL;DR: Computer programs have been developed in order to perform qualitative and quantitative analyses of autoradiographs obtained with the 2-DG method, which are written in FORTRAN and MACRO 11 Assembly Language.

TL;DR: A recently developed small controlled-delivery peptide device has been further miniaturized and its action has been tested in vitro and in vivo using vasopressin (VP) as experimental substance and a steadily enhanced level of VP could be attained for CSF.