Showing papers in "Steroids in 1963"

TL;DR: It is concluded that the placenta does not exclusively utilize de novo mechanisms for estrogen production and may, in fact, rely entirely upon externally supplied steroidal precursors.

TL;DR: A gradient elution, liquid-liquid partition Chromatographic system has been devised which is capable of isolating in purified form most of the estrogens present in crude phenolic extracts from 10 or more liters of human urine.

TL;DR: A soluble cholesterol side-chain cleaving enzyme system has been prepared from bovine corpus luteum which can be stimulated in vitro by added gonadotrophins and appears to exert their effect on the 20α-hydroxylation of cholesterol.

TL;DR: The use of heptafluorobutyrates as derivatives for the ultra-sensitive measurement of hydroxyl containing steroids and using an electron affinity detector has been described.

TL;DR: In this article, a method was developed for the analysis of testosterone in human urine, where one tenth of a 24 hour urine sample was incubated with β-glucuronidase, and extracted with ethyl ether, the ketonic neutral fraction was subjected to thin layer chromatography and the eluted testosterone zone was analyzed gas chromatographically employing a stainless steel column containing 6.8% SE-30 Anakrom ABS (110-120) mesh and a flame ionization detector.

TL;DR: The thesis is advanced that the 19-nor androgens may well be the active androgens at the end organ, that they in turn give rise to the estrogens, and that testosterone merely serves as a precursor of the active 19-Nor steroids.

TL;DR: A simple method based on isotope dilution and double thin layer chromatography has been developed for the determination of testosterone in human urine, with precision of the order of 1.7 μg, the sensitivity 4 μ/24 hrs, and the maximum error 10%.

TL;DR: H3-dehydroisoandrosterone-S35-sulfate was isolated from the urine, demonstrating a “direct” (without splitting of the ester link) in vivo oxidation of a steroid conjugate.

TL;DR: Another metabolite, in which ring A appears not to have been metabolized, was isolated but not as yet finally identified and was isolated from the urine of a patient after administration of Δ 1 ,17α-methyltestosterone.

TL;DR: In this article, the effects of (±)-13β, 17α-diethyl-17β-hydroxygon-4-en-3-one were compared with resolved samples of the component enantiomers.

TL;DR: It is shown that storage in and release from body fat is the mechanism responsible for the increased oral activity exhibited by ethynyl estradiol 3 cyclopentyl ether (EECFE), which could explain the prolonged estrogenic effect observed in menopausal patients after oral administration of EECPE was discontinued.

TL;DR: The mass spectra of a variety of steroidal ethylene ketals and ethylene thioketals have been studied in this article, and the results indicate that these functions strongly direct fragmentation in a predictable manner.

TL;DR: It was concluded that “gamma-sitosterol” reportedly obtained from other sources is most likely a campesterol-beta-Sitosterol mixture, and gas-liquid chromatographic analyses showed that the material consists of a 50:50 to a 75:25 mixture of campestersol- beta-sitosterone.

TL;DR: The proportion of 20 α-hydroxypregn-4-en-3-one to progesterone shows a peak in the proestrus and estrus phases, which may be regarded as a consequence of altered 20α-hydroxysteroid dehydrogenase activity in the ovarian tissue.

TL;DR: In this paper, it was shown that Δ 1 - and Δ 4 -3-keto steroids exhibit a characteristic mass spectral peak in the high mass range at M-42, corresponding to the loss of the carbonyl function and the adjacent methylene grouping.

TL;DR: Since 13beta-ethyl-17alpha-ethynl-17beta-hydroxygon-4-en-3-one is a potent progestational agent with marked oral activity and relatively little androgenicity it would warrant examination as a therapeutic agent.

TL;DR: Neither of the estrogens could be isolated from urine of a patient with secondary amenorrhea with increased PSH excretion, nor from a patients with bilateral ovarieotomy, in both cases the fluorometric values were close to zero.

TL;DR: A simple rapid method for the separation and quantitative determination of urinary 17-hydroxycorticosteroids is described, which is carried out by Portet — Silber reaction.

TL;DR: A detailed NMR investigation of a number of steroidal sapogenins in the 25D and 25L series has been carried out as discussed by the authors, utilizing deuteriochloroform and pyridine solutions, has revealed that NMR can provide direct evidence regarding the stereochemical nature of C25.

TL;DR: A “direct” 17β-hydroxyl pathway, without 17-ketonic intermediates, was demonstrated in vivo and the hypothesis that in addition to the androst-5-ene-3β,17β-diol → testosterone → “diols” pathway, another sequence of 17 β-hydroxy metabolites exists exists was proposed.

TL;DR: In this paper, a procedure for the isolation and purification of 28 C(19)-steroids in milligram quantities was described, using a "marker chromatostrip".

TL;DR: The use of two new stationary phases for high temperature gas chromatography has allowed the simultaneous separation of the principal 17-ketosteroids and progesterone metabolites present in human urine as mentioned in this paper.

TL;DR: In this paper, the conversion of 5α-substituted 6β,19-oxides into δ4-3-keto-6β, 19oxides and lactones in the androstane series is described.

TL;DR: It is concluded that, because of the very low levels of radioactivity remaining in the cholesterol and the inability to obtain a constant specific activity throughout the purification process, there was no significant incorporation of 1−14C acetate into cholesterol by the firebrat.

TL;DR: Using a similar test, employing testosterone as the androgen, anti-androgenic activity, as judged by both the weights of the prostate and seminal vesicles, was found for l6β-methyl-5α-dihydrotestololactone, 6-dibromomethylene-testosterone propionate, 17,17-dimethylgona-4,13-dien-3-one, and 17, 17-dim methyl-