Journal ArticleDOI
A 13C comparative nuclear magnetic resonance study of organic solute production and excretion by the yeasts Hansenula anomala and Saccharomyces cerevisiae in saline media.
Yves Bellinger,François Larher +1 more
TLDR
Glycerol, arabitol and trehalose were the principle solutes detected in cellular extracts of Hansenula anomala, using natural-abundance 13C nuclear magnetic resonance spectroscopy, and the former displayed the specific property of increasing this capacity in high salt concentrations.Abstract:
Glycerol, arabitol and trehalose were the principle solutes detected in cellular extracts of Hansenula anomala, using natural-abundance 13C nuclear magnetic resonance spectroscopy. Only the two polyols accumulated in response to increased salinity, glycerol increase being far greater. Arabitol content also increased with culture age, independently of the presence or absence of salt and in line with the evolution of trehalose content. Glycerol retention potential was 15 times greater for Hansenula than for Saccharomyces cerevisiae. The former displayed the specific property of increasing this capacity in high salt concentrations. Under such conditions its growth was associated with a limited increase in glucose consumption per unit biomass, relative to S. cerevisiae, the salt-sensitive reference yeast. In addition, a polysaccharide, the chemical nature of which was not further characterized, was detected exclusively in the external medium of Hansenula growing in the presence of salt.read more
Citations
More filters
Journal ArticleDOI
Bacterial osmoadaptation: the role of osmolytes in bacterial stress and virulence
Roy D. Sleator,Colin Hill +1 more
TL;DR: The molecular mechanisms governing the accumulation of these compounds, both in Gram-positive and Gram-negative bacteria, are reviewed, focusing specifically on the regulation of their transport/synthesis systems and the ability of these systems to sense and respond to changes in the osmolarity of the extracellular environment.
Book ChapterDOI
Physiology of osmotolerance in fungi
Anders Blomberg,Lennart Adler +1 more
TL;DR: Combined genetic and physiological analysis is required for a deeper understanding of fungus-water relations and has revealed sequential induction of osmotically controlled genes in enteric bacteria and given exciting insights in signal transduction and regulation of the process.
Journal ArticleDOI
Osmoregulation and glycerol metabolism in the yeast Saccharomyces cerevisiae
Elke Nevoigt,Ulf Stahl +1 more
TL;DR: Current knowledge concerning mechanisms leading to the accumulation of glycerol in osmotically stressed cells of S. cerevisiae is evaluated at the molecular and metabolic levels.
Journal ArticleDOI
Biotechnology, physiology and genetics of the yeast Pichia anomala.
TL;DR: The ascomycetous yeast Pichia anomala is frequently associated with food and feed products, either as a production organism or as a spoilage yeast, which makes it a potential agent to decrease environmental pollution by organic residues from agriculture.
Journal ArticleDOI
A multi-level study of recombinant Pichia pastoris in different oxygen conditions
Kristin Baumann,Marc Carnicer,Martin Dragosits,Alexandra B. Graf,Johannes Stadlmann,Paula Jouhten,Hannu Maaheimo,Brigitte Gasser,Joan Albiol,Diethard Mattanovich,Pau Ferrer +10 more
TL;DR: This systems level study helped to understand the physiological adaptations of cellular mechanisms to low oxygen availability in a recombinant P. pastoris strain, yielding clear evidence of a strong transcriptional regulation component of key pathways such as glycolysis, pentose phosphate pathway and TCA cycle.