Journal ArticleDOI
A detailed method for obtaining preparations of human sperm chromosomes.
TLDR
A detailed technique is described for obtaining preparations of the chromosome complements of human sperm by fertilization of hamster eggs and analysis of the male pronucleus.Abstract:
A detailed technique is described for obtaining preparations of the chromosome complements of human sperm by fertilization of hamster eggs and analysis of the male pronucleus. Some of the more difficult aspects and important steps are emphasized. Technical data from 17 consecutive experiments are presented to provide an estimate of the number of karyotypes which can be obtained in an experiment.read more
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Journal ArticleDOI
Human male infertility: chromosome anomalies, meiotic disorders, abnormal spermatozoa and recurrent abortion
S. Egozcue,Joan Blanco,J.M. Vendrell,F. García,Anna Veiga,B. Aran,Pere N. Barri,Francesca Vidal,J. Egozcue +8 more
TL;DR: In this article, the rates of chromosome 21 and sex chromosome disomy in spermatozoa are increased in normal infertile males, indicating an increased risk of production of XXY, XYY and XXX individuals.
Journal ArticleDOI
Zona‐free hamster eggs: Their use in assessing fertilizing capacity and examining chromosomes of human spermatozoa
Journal ArticleDOI
Chromosomal analysis of sperm from men with idiopathic infertility using sperm karyotyping and fluorescence in situ hybridization
Nafisa Moosani,H.A. Pattinson,Michael D. Carter,David M. Cox,Alfred Rademaker,Renee H. Martin +5 more
TL;DR: Sperm from infertile men may contain an increased frequency of chromosomal abnormalities, as determined by sperm karyotypes and the disomy frequency determined by fluorescence in situ hybridization analysis.
Journal ArticleDOI
Andrology: Analysis of chromosome constitution of human spermatozoa with normal and aberrant head morphologies after injection into mouse oocytes
TL;DR: Results indicate that some morphological abnormalities in the sperm heads are associated with their chromosome defects, and this is similar to those found in previous studies using the hamster oocyte-human sperm fusion system.
Journal ArticleDOI
Turner syndrome revisited: review of new data supports the hypothesis that all viable 45,X cases are cryptic mosaics with a rescue cell line, implying an origin by mitotic loss
Ernest B. Hook,Dorothy Warburton +1 more
TL;DR: All 45, X individuals with Turner syndrome are cryptic mosaics, absence of the X chromosome in 45,X embryos is caused primarily by mitotic factors, and the placenta is a strong candidate for the location of the rescue line in apparently non-mosaic 45,Z individuals.
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