Journal ArticleDOI
A direct agglutination test discriminative toward Chagas' disease for the diagnosis of visceral leishmaniasis in Brazil: preliminary results.
Reads0
Chats0
TLDR
The assay proved to be of value for the diagnosis of American visceral leishmaniasis in that it was discriminative toward Chagas' disease.Abstract:
Summary Two-hundred and sixty-five human sera from Brazil were tested in a direct agglutination assay using trypsinated Leishmania donovani donovani antigen. The assay proved to be of value for the diagnosis of American visceral leishmaniasis in that it was discriminative toward Chagas' disease.read more
Citations
More filters
Journal ArticleDOI
Improvement of a direct agglutination test for field studies of visceral leishmaniasis.
TL;DR: The test could be performed on samples of whole blood; thus the difficulties of preparation and storage of serum, plasma, or filter paper blood are avoided and a single serum dilution of 1:6,400 could be employed, contributing to a further reduction in test expenses.
Journal ArticleDOI
Serodiagnosis of Leishmaniasis
TL;DR: The use of whole promastigote as the source of antigens in the direct agglutination test (DAT) and immunofluorescent test (IFAT) gave cross-reactions with the sera of leprosy, tuberculosis, and African trypanosomiasis patients.
Journal ArticleDOI
Application of a direct agglutination test for detection of specific anti-Leishmania antibodies in the canine reservoir.
A el Harith,R J Slappendel,I Reiter,F van Knapen,P de Korte,Elizabeth Huigen,Arend H. J. Kolk +6 more
TL;DR: The results of this study demonstrate that the DAT is highly suitable for wide-scale epidemiological and ecological field work and could also facilitate diagnosis of leishmaniasis in dogs in veterinary health services.
Journal ArticleDOI
Comparison of serological assays for the diagnosis of canine visceral leishmaniasis in animals presenting different clinical manifestations
Eduardo de Castro Ferreira,Marta de Lana,Mariângela Carneiro,Alexandre Barbosa Reis,Daniela Vieira Paes,Eduardo Silva,Henk D. F. H. Schallig,Célia Maria Ferreira Gontijo +7 more
TL;DR: Three serological methods, indirect fluorescent immunoassay (IFI), enzyme-linked immunosorbent assay (ELISA) and direct agglutination test (DAT) that are commonly employed in the diagnosis of canine visceral leishmaniasis (CVL), have been assessed.
Journal ArticleDOI
Leish-KIT, a stable direct agglutination test based on freeze-dried antigen for serodiagnosis of visceral leishmaniasis.
S.E.O. Meredith,Nel C. M. Kroon,E. Sondorp,J. Seaman,Marga G. A. Goris,C. W. Van Ingen,H. Oosting,Gerard J. Schoone,W. J. Terpstra,Linda Oskam +9 more
TL;DR: It was shown that the freeze-dried antigen remains fully active, even after storage at 56 degrees C for 18 months, which makes the direct agglutination test for the detection of anti-Leishmania antibodies in human serum samples a valuable diagnostic tool for use in the field.
References
More filters
Journal ArticleDOI
A simple and economical direct agglutination test for serodiagnosis and sero-epidemiological studies of visceral leishmaniasis.
TL;DR: A simple and economical direct agglutination test for the detection of visceral leishmaniasis is described and could be used in district hospitals and health centres in endemic areas as an aid in diagnosis of kala-azar and in the field for sero-epidemiological studies.
Journal ArticleDOI
Subspecies-specific surface antigens of promastigotes of the Leishmania donovani complex.
TL;DR: It is demonstrated that the surface antigenic expression of L. infantum is different from that noticed in the two other subspecies, which are similar, and should be considered in taxonomic and immunological studies in visceral leishmaniasis.