Journal ArticleDOI
A staining combination for phloem and contiguous tissues.
TLDR
The technic has been successful with sections from 5 to 40μ in thickness, and the staining has been satisfactory for ...Abstract:
A technic is described for producing critically stained preparations of phloem tissue. The preparations promise to be relatively stable. Sections of fixed unembedded or of embedded (paraffin or celloidin) phloem, cambium, and xylem are (1) stained in Foster's tannic acid-ferric chloride combination; (2) treated with 1% NaHCOg in 25% or 50% ethyl alcohol for 30 minutes; (3) stained in a saturated solution of lacmoid (made alkaline by adding a few ml. of 1% NaHCO3 in 25% alcohol) for 12 to 18 hours; (4) dehydrated and cleared in a series composed of 1% solution of NaHCOs in 50% ethyl alcohol, 80%, 95%, and absolute alcohol, equal proportions of absolute alcohol, clove oil, and xylene, and finally pure xylene; and (5) mounted in a neutral resin. Callose and lignified secondary walls are blue or blue-green in color, cellulose walls and stainable protoplasmic contents are generally light brown. The technic has been successful with sections from 5 to 40μ in thickness, and the staining has been satisfactory for ...read more
Citations
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Journal ArticleDOI
Callose substance in plant cells
TL;DR: Interest in callose has been displayed by students of phloem since sieve tubes contain an abundance of the substance, and by plant pathologists because the mycelial walls of many fungi contain callose.
Journal ArticleDOI
Identification of Cauose by its Diachrome and Fluorochrome Reactions
W. Eschrich,H. B. Currier +1 more
TL;DR: Methods for callose deposits can be applied to both living and fixed plant tissue, because the stains required are relatively nontoxic and may be added to incubating media.
Journal ArticleDOI
Experimental induction of vascular tissues in callus of angiosperms
TL;DR: Experimental induction of vascular tissues has been successful in callus of 3 sp.
Journal ArticleDOI
Aniline blue and fluorescence microscopy of callose in bulb scales ofAllium cepa L
H. B. Currier,Siegfried Strugger +1 more
TL;DR: In this article, the fluorescence-microscopic method is used in which substances of callose nature appear yellow when stained with water-soluble aniline blue, and the formation of calloses, as judged by intensity of fluorescence, is increased by wound stimulation.
References
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Journal ArticleDOI
The Use of Tannic Acid and Iron Chloride for Staining Cell Walls in Meristematic Tissue
TL;DR: The use of Tannic acid and iron chloride for staining cell walls in Meristematic Tissue was discussed in this article, where the authors show that TANNIC acid and IR can be used to stain cell walls.
Journal ArticleDOI
Phloem structure in the grapevine and its seasonal changes
TL;DR: The present article deals with the phloem of plants that showed no symptoms of any disease, which is a background for a study of the anatomical effects of the virus-induced Pierce's disease upon this plant.
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Softening Refractory Plant Material Embedded in Paraffin
TL;DR: In this article, Softening Refractory Plant Material Embedded in Paraffin (SRLPMEM) is presented as a softening refractory plant material embedded in paraffin.
Journal ArticleDOI
The use of Celloidin in Botanical Technic
TL;DR: The use of Celloidin in Botanical Technic was discussed in this paper, where the authors propose a method to use the celloidin as a stain technology for plant growth.
Journal ArticleDOI
Improvements in the paraffin method.
TL;DR: Dilute hydrofluoric acid alone and in conjunction with glycerin and ethyl alcohol was employed successfully to soften various types of refractory plant materials embedded in paraffin to remove tannins andphlobaphene compounds.