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A test for de novo synthesis of enzymes: density labeling with H2O18 of barley alpha-amylase induced by gibberellic acid.

Philip Filner, +1 more
- 01 Oct 1967 - 
- Vol. 58, Iss: 4, pp 1520-1526
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This article is published in Proceedings of the National Academy of Sciences of the United States of America.The article was published on 1967-10-01 and is currently open access. It has received 258 citations till now. The article focuses on the topics: Gibberellic acid & De novo synthesis.

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Journal ArticleDOI

The Structure of Plant Cell Walls: I. The Macromolecular Components of the Walls of Suspension-cultured Sycamore Cells with a Detailed Analysis of the Pectic Polysaccharides.

TL;DR: The structures of the pectic polymers (the neutral arabinan, the neutral galactan, and the acidic rhamnogalacturonan) were obtained by methylation analysis of fragments of these polymers which were released from the sycamore walls by the action of a highly purified endopolygalacturonase.
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The Arabidopsis Aleurone Layer Responds to Nitric Oxide, Gibberellin, and Abscisic Acid and Is Sufficient and Necessary for Seed Dormancy

TL;DR: Molecular changes that occurred in embryos and aleurone layers prior to germination were measured, and these data show that both the aleur one layer and the embryo expressed the NO-associated gene AtNOS1, but only the embryos expressed genes for the GA biosynthetic enzyme GA3 oxidase.
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Proteomic analysis of rice (Oryza sativa) seeds during germination

TL;DR: A proteomic analysis of seed germination in rice showed that there were 148 proteins displayed differently in the germination process of rice seeds, and the results reflected the possible biochemical and physiological processes of germination of Rice seeds.
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Structure of Plant Cell Walls: XIX. Isolation and Characterization of Wall Polysaccharides from Suspension-Cultured Douglas Fir Cells

TL;DR: The cell walls of Douglas fir were more similar to dicot (sycamore) cell walls than to those of graminaceous monocots, because they had a predominance of xyloglucan over xylan as the principle hemicellulose and because they possessed relatively large amounts of rhamnogalacturonan-like pectic polysaccharides.
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An early response to gibberellic Acid not requiring protein synthesis.

TL;DR: It is concluded that the GA(3)-dependent increase in phosphorylcholine glyceride transferase activity (which occurs within the first 4 hours ofGA(3) treatment) does not require RNA synthesis or protein synthesis.
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