Open AccessJournal Article
Accumulation of anionic pesticides by rabbit choroid plexus in vitro.
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TLDR
Transport of the serotonin metabolite, 5-hydroxy-3-indole-acetic acid was more effectively inhibited by these pesticides than by the classical inhibitor, probenecid, neither the parent amine, serotonin, nor the uncharged pesticides.Abstract:
In this study, two questions were addressed: 1) were anionic pesticides accumulated by choroid plexus via the organic acid transport system and 2) did such accumulation impair transport of anionic neurotransmitter metabolites normally eliminated from brain via this system? Rabbit lateral choroid plexi were incubated in Krebs-Ringer bicarbonate buffer in vitro with the herbicide, 2,4-dichlorophenoxyacetic acid, or the polar DDT metabolite, 2,2-bis (p-chlorophenyl)acetic acid (DDA). Both compounds were extensively accumulated. At a pesticide concentration of 1 microM, tissue-to-medium ratios of 40 (DDA) to 50 (2,4-dichlorophenoxyacetic acid) were found after 60-min incubation at 37 degrees C. Uptake of both compounds was energy- and concentration-dependent and could be inhibited by other organic anions. 2,4-Dichlorophenoxyacetic acid and DDA each produced dose-dependent inhibition of the uptake of the other pesticide. DDA was the more potent inhibitor. Thus, uptake of both compounds appeared to be mediated by the organic acid transport system. Furthermore, transport of the serotonin metabolite, 5-hydroxy-3-indole-acetic acid was more effectively inhibited by these pesticides than by the classical inhibitor, probenecid. Neither the parent amine, serotonin, nor the uncharged pesticides. DDT and dieldrin, inhibited 5-hydroxy-3-indole-acetic acid uptake. Thus, the effect appears specific for those compounds which share the organic anion transport system.read more
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A Comprehensive, Integrated Review and Evaluation of the Scientific Evidence Relating to the Safety of the Herbicide 2,4-D
Ian C. Munro,George L. Carlo,Joan C. Orr,Kelly G. Sund,Ross M. Wilson,Elke Kennepohl,Barry Lynch,Maureen R. Jablinske +7 more
TL;DR: The available data indicate that the potential public health impact of 2,4-D, including the risk of human cancer, was negligible in the past and would be expected to be even smaller in the present and future.