Fig. 1 Mitochondrial accumulation of FlAsH/Lumio Green and toxic side effects a Untransfected N2a cells were labeled with 1 lM FlAsH/ Lumio Green and Mitotracker Orange in the absence of thiolcontaining reagents for 1 h, washed and analyzed by fluorescence microscopy. FlAsH/Lumio Green clearly accumulated in mitochondria stained by MitoTracker (a confocal slice acquired by structured illumination is shown). b Many cells stained as described above exhibited aberrant mitochondria with a round, swollen appearance c Untransfected N2a cells were stained with 1 lM FlAsH/ Lumio Green + 4 lM 2-ME for 1 h, washed and incubated with 100 lM 2-ME for 30 min. This treatment reversed mitochondrial accumulation of FlAsH/Lumio Green and resulted in a diffuse background staining. d Cells were labeled with 1 lM FlAsH/Lumio Green for 1 h, washed and then incubated with 5 lM rotenone for 15 min. Inhibition of mitochondrial respiration by rotenone also reversed the mitochondrial accumulation of FlAsH/Lumio Green fluorescence. All bars, 10 lm
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