Open AccessJournal Article
Activation of Normal Rabbit Macrophage Monolayers by Supernatants of Antigen-Stimulated Lymphocytes
John J. Mooney,Byron H. Waksman +1 more
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Macrophages exposed to supernatants from mixtures of sensitized cells with specific antigen had two to five times greater numbers of ameboid cells at 24 and 48 hr than did monolayers of the same macrophage exposed to control supernatant, and twice as many cells remained adherent at 48 hr.Abstract:
Tuberculin- or HSA-sensitized rabbit lymph node cells, harvested at 10 to 14 days, and normal rabbit lymph node cells were incubated for 24 hr alone or in the presence of specific or heterologous antigen. The supernatants prepared from these lymph node cell cultures were added to duplicate or triplicate monolayer cultures of peritoneal macrophages from normal rabbits, each macrophage being exposed to soluble products released in vitro by 10 lymph node cells. After 24 and 48 hr of continuous exposure to the undiluted supernatants, 150 to 300 cells in each flask were scored for adherence and ameboid activity. Macrophages exposed to supernatants from mixtures of sensitized cells with specific antigen had two to five times greater numbers of ameboid cells at 24 and 48 hr than did monolayers of the same macrophages exposed to control supernatants, and twice as many cells remained adherent at 48 hr. Thus, factors released by sensitized lymphocytes cause activation of normal macrophages. It is suggested that a similar mechanism may be responsible for cell-mediated immunity in systems involving microbial antigens.read more
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Book ChapterDOI
In Vitro Approaches to the Mechanism of Cell-Mediated Immune Reactions
TL;DR: This chapter focusses on the study of cell-mediated immunity in vitro, principally in terms of explaining the effector process of the response, with general assumptions that all cell- mediated immune reactions have a common basis.
Journal ArticleDOI
Characterization of a lymphocyte factor which alters macrophage functions.
TL;DR: The data suggests that the activating factor is the same as MIF and that, in vitro, macrophages respond to this substance with migration inhibition before they become sensitive to its activating influence.
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Glucocorticoid suppression of macrophage migration inhibitory factor.
James E. Balow,Alan S. Rosenthal +1 more
TL;DR: Hydrocortisone antagonized directly the inhibitory effect of MIF on the macrophage, and had no effect on antigen "processing" by macrophages, nor on the ability of antigen-stimulated peritoneal exudate lymphocytes to produce MIF.
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The enhancement of macrophage bacteriostasis by products of activated lymphocytes.
TL;DR: Macrophages, which have been preincubated directly with sensitive lymphocytes and antigen exhibit even greater bacteriostasis and sometimes bactericidal capacity, suggesting that either a labile lymphocyte factor or direct lymphocyte macrophage interaction may also be involved in bactericidal activity.
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Current status of the host response in chronic marginal periodontitis.
Roy C. Page,Hubert E. Schroeder +1 more
TL;DR: The role of bacteria in the pathogenesis of Periodontitis was discussed in a series of papers beginning in 1920 by Rehnwinkel et al. as discussed by the authors, with the focus shifting away from bacteria and toward systemic host factors.
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