Journal ArticleDOI
Mechanism of a Reaction in Vitro Associated with Delayed-Type Hypersensitivity
Barry R. Bloom,Boyce Bennett +1 more
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The cell type responsible for inhibition by antigen of migration in vitro of peritoneal exudate cells obtained from tuberculin-hypersensitive guinea pigs was studied and elaborated into the medium a soluble material capable of inhibiting migration of normal exudates.Abstract:
The cell type responsible for inhibition by antigen of migration in vitro of peritoneal exudate cells obtained from tuberculin-hypersensitive guinea pigs was studied. Exudate populations were separated into component cell types, the lymphocyte and the macrophage. Peritoneal lymphocytes from sensitive donors were the immunologically active cells in this system, the macrophages, being merely indicator cells which migrate. Sensitized peritoneal lymphocyte populations, upon interaction with specific antigen in vitro, elaborated into the medium a soluble material capable of inhibiting migration of normal exudate cells.read more
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Disseminated tuberculosis in interferon gamma gene-disrupted mice.
Andrea M. Cooper,Dyana K. Dalton,Timothy A. Stewart,J P Griffin,David G. Russell,Ian M. Orme +5 more
TL;DR: It is shown that mice in which the IFN-gamma gene has been disrupted were unable to contain or control a normally sublethal dose of M. tuberculosis, delivered either intravenously or aerogenically, and that despite the lack of protective immunity, some DTH-like reactivity could still be elicited.
Journal ArticleDOI
The role of tumour‐associated macrophages in tumour progression: implications for new anticancer therapies
TL;DR: Evidence for the number and/or distribution of TAMs being linked to prognosis in different types of human malignancy is presented and the range of pro‐ and anti‐tumour functions performed by TAMs are outlined, and the novel therapies recently devised using TAMs to stimulate host immune responses or deliver therapeutic gene constructs to solid tumours are outlined.
Journal ArticleDOI
Macrophage migration inhibitory factor: a regulator of innate immunity.
Thierry Calandra,Thierry Roger +1 more
TL;DR: A rapidly increasing amount of literature indicates that Mif is implicated in the pathogenesis of sepsis, and inflammatory and autoimmune diseases, suggesting that MIF-directed therapies might offer new treatment opportunities for human diseases in the future.
Journal ArticleDOI
MIF as a glucocorticoid-induced modulator of cytokine production
Thierry Calandra,Jürgen Bernhagen,Christine N. Metz,Lori Spiegel,Michael Bacher,Thomas M. Donnelly,Anthony Cerami,Richard Bucala +7 more
TL;DR: The unexpected finding that low con-centrations of glucocorticoids induce rather than inhibit MIF production from macrophages is reported, identifying a unique counter-regulatory system that functions to control inflammatory and immune responses.
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MIF is a pituitary-derived cytokine that potentiates lethal endotoxaemia
Jürgen Bernhagen,Thierry Calandra,Robert A. Mitchell,S. B. Martin,Kevin J. Tracey,Wolfgang Voelter,Kirk R. Manogue,A. Cerami,Richard Bucala +8 more
TL;DR: Macrophage migration inhibitory factor (MIF) is identified as a major secreted protein released by anterior pituitary cells in response to LPS stimulation, and it is concluded that MIF plays a central role in the toxic response to endotoxaemia and possibly septic shock.
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Journal Article
Delayed hypersensitivity in vitro. i. the specificity of inhibition of cell migration by antigens.
TL;DR: The peritoneal exudate cells from guinea pigs with delayed hypersensitivity to tuberculin purified protein derivative, ovalbumin and diphtheria toxoid are markedly inhibited by the respective antigen, and such inhibition is specific as discussed by the authors.
Journal ArticleDOI
In vitro cell migration as a model for delayed hypersensitivity.
Mariam George,John H. Vaughan +1 more
TL;DR: The ability of antigen to inhibit cell migration in tissue culture has been confirmed with peritoneal exudate cells for tuberculin hypersensitivity and this in vitro characteristic has been shown to apply also to the delayed hypersensitivity that develops after immunization with a protein antigen in Freund's adjuvant.
Journal Article
Delayed Hypersensitivity in vitro. I. The Specificity of Inhibition of Cell Migration by Antigens.
TL;DR: The migration of peritoneal exudate cells from guinea pigs with delayed hypersensitivity to tuberculin purified protein derivative, ovalbumin and diphtheria toxoid is markedly inhibited by the respective antigen, and such inhibition is specific.
Journal Article
Delayed Hypersensitivity in Vitro II. Effect of Sensitive Cells on Normal Cells in the Presence of Antigen
David,Lawrence Hs,Thomal L +2 more
TL;DR: The results indicate that only a few cells in a mixed population need be specifically sensitive to influence the behavior of the whole population, and killing the sensitive cells abolishes their effect on normal cells.
Journal ArticleDOI
Homograft target cells: specific destruction in vitro by contact interaction with immune macrophages.
TL;DR: Cell destruction apparently resulted from a nonphagocytic mechanism involving cell contact rather than humoral antibody in contact in vitro of immune peritoneal macrophages from C57BI/6K mice with homograft target cells from A/Jax mice.