Journal ArticleDOI
Ammonia determines the choice of morphogenetic pathways in dictyostelium discoideum
Joel Schindler,Maurice Sussman +1 more
TLDR
Evidence is presented to support the conclusion that the metabolite in question is NH3, and the biological and biochemical performances of autoinduced slugs and of those induced by the metabolismite preparations or by ammonium carbonate solutions were indistinguishable.About:
This article is published in Journal of Molecular Biology.The article was published on 1977-10-15. It has received 181 citations till now. The article focuses on the topics: Cell aggregation & Metabolite.read more
Citations
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Book ChapterDOI
Cultivation and synchronous morphogenesis of Dictyostelium under controlled experimental conditions.
TL;DR: Under optimal conditions, vegetative cell populations harvested from nutrient medium and dispensed homogeneously on a solid substratum can be made to exhibit remarkably consistent morphogenetic behavior in respect to the timing and patterns of aggregation and of fruiting body construction.
Journal ArticleDOI
Dictyostelium discoideum: a model system for cell-cell interactions in development
TL;DR: A method of cell-cell signaling that controls chemotaxis, morphogenesis, and gene expression has developed in this organism, and a detailed understanding of this signaling system provides clues to mechanisms of intercellular communication in the development of metazoans.
Journal ArticleDOI
Intracellular pH and the control of cell differentiation in Dictyostelium discoideum
TL;DR: Evidence is presented that ammonia is a second morphogen, that acts antagonistically to DIF, and that the choice of differentiation pathway is mediated by intracellular pH.
Journal ArticleDOI
An analysis of culmination in Dictyostelium using prestalk and stalk-specific cell autonomous markers.
K.A. Jermyn,J.G. Williams +1 more
TL;DR: The ecmA and ecmB genes are expressed at a low level in ALC during slug migration and that their expression in these cells is greatly elevated during culmination, and expression of the ecmA gene becomes uniformly high throughout the prestalk zone when slugs are allowed to migrate in the light.
Journal ArticleDOI
Integration of signaling networks that regulate Dictyostelium differentiation.
Laurence Aubry,Richard A. Firtel +1 more
TL;DR: This review focuses on the role of cAMP, which functions intracellularly to mediate the activity of PKA, an essential component in aggregation, cell-type specification, and terminal differentiation in Dictyostelium development.
References
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Book ChapterDOI
Chapter 14 Biochemical and Genetic Methods in the Study of Cellular Slime Mold Development
TL;DR: This chapter describes genetic, biochemical, and immunochemical techniques that have been successfully applied in the laboratory to the study of cellular slime mold development.
Journal ArticleDOI
Control of cell-contact sites by cyclic AMP pulses in differentiating Dictyostelium cells.
TL;DR: The appearance of contact sites A is used to test the hypothesis that cyclic AMP is a signal for initiating differentiation6,7, as well as acting as the aggregation signal for differentiated cells.
Journal ArticleDOI
Alternative developmental pathways determined by environmental conditions in the cellular slime mold Dictyostelium discoideum
TL;DR: The cellular slime mold Dictyostelium discoideum grows in the soil as a population of independent, uninucleate amoebae that collect in multicellular aggregates to form organized fruiting bodies composed of spores and stalk cells.
Journal ArticleDOI
The orientation to light and the extermely sensitive orientation to temperature gradients in the slime mold Dictyostelium discoideum
Journal ArticleDOI
Cultivation of Dictyostelium discoideum in axenic medium
R. Sussman,M. Sussman +1 more