Journal ArticleDOI
Anion selectivity of the chloride-transport process in dog tracheal epithelium
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The results show that the affinity sequence for the chloride transport process is I > Br congruent to Cl, that Br is transported, but that I is not, and that thiocyanate is a potent inhibitor of chloride transport.Abstract:
We have measured the fluxes of 82Br or 131I across short-circuited dog tracheal epithelium following complete replacement of chloride in the bathing medium by either bromide or iodide. In addition, we have partly replaced the bathing chloride with bromide, iodide, or thiocyanate and studied the effects of these anions on 36Cl fluxes. Our results show that the affinity sequence for the chloride transport process is I > Br congruent to Cl, that Br is transported, but that I is not. Thiocyanate is a potent inhibitor of chloride transport, and its effects are greater than those predicted by a simple competitive interaction with chloride for transport sites.read more
Citations
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Transepithelial transport by pulmonary alveolar type II cells in primary culture.
Robert J. Mason,Mary C. Williams,Jonathan Widdicombe,Martin J. Sanders,Dayton S. Misfeldt,Leonard C. Berry +5 more
TL;DR: Alveolar type II cells form a polarized epithelium in culture, and these cells actively transport electrolytes in vitro according to the electrical properties of monolayer cultures.
Journal ArticleDOI
Regulation of chloride channels by protein kinase C in normal and cystic fibrosis airway epithelia.
Ming Li,John D. McCann,Matthew P. Anderson,John Clancy,Carole M. Liedtke,Angus C. Nairn,Paul Greengard,Michael J. Welsch +7 more
TL;DR: In cystic fibrosis cells, PKC-dependent channel inactivation was normal, but activation was defective, and it appears that PKC phosphorylates and regulates two different sites on the channel or on an associated membrane protein, one of which is defective in cystic Fibrosis.
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Erythromycin inhibits Cl secretion across canine tracheal epithelial cells.
TL;DR: The results indicate that erythromycin may selectively inhibit Cl secretion across airway epithelium through the inhibition of prostaglandin synthesis and suggest that this action possibly reflects its clinical efficacy in the treatment of airway hypersecretion.
Journal ArticleDOI
Anion permeation in an apical membrane chloride channel of a secretory epithelial cell
Dan R. Halm,Raymond A. Frizzell +1 more
TL;DR: Results indicate that this 40-pS Cl channel behaves as a multi-ion pathway in which other permeant anions could alter Cl flow across the apical membrane.
Journal ArticleDOI
Anion selectivity of apical membrane conductance of Calu 3 human airway epithelium.
TL;DR: Cystic fibrosis conductance transmembrane conductance regulator appears to be the major apical anion conductance pathway in Calu-3 cells, and its conduction properties are independent of the expression system.
References
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Journal ArticleDOI
Active transport of sodium as the source of electric current in the short-circuited isolated frog skin.
Hans H. Ussing,K. Zerahn +1 more
Journal ArticleDOI
Anion selectivity in biological systems.
Ernest M. Wright,J. M. Diamond +1 more
TL;DR: Four theoretical models to account for the observed anion potency sequences as sequences of equilibrium binding energies are developed and the reasons why this conclusion is not as paradoxical as it at first seems are discussed.
Journal ArticleDOI
Coupled sodium-chloride influx across the brush border of rabbit ileum.
Journal ArticleDOI
Chloride transport across isolated opercular epithelium of killifish: a membrane rich in chloride cells
TL;DR: The opercular epithelium of Fundulus heteroclitus contains typical gill chloride-secreting cells at the high density of 4 X 10(5) cells per square centimeter, which offers a useful approach to the study of osmoregulation in bony fishes.